『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨): 1.生命情報工学 (2006年4月1日〜2016年3月31日) [継承]
著者 (必須): 1.湯浅 恵造 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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[継承]
2. (英) Futamatsu Go (日) (読)
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[継承]
3. (英) Kawano Tsuyoshi (日) (読)
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[継承]
4. (英) Muroshita Masaki (日) (読)
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5. (英) Kageyama Yoko (日) (読)
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[継承]
6. (英) Taichi Hiromi (日) (読)
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[継承]
7. (英) Ishikawa Hiroshi (日) (読)
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[継承]
8. (英) Nagahama Masami (日) (読)
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[継承]
9. (英) Matsuda Yoshiko (日) (読)
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[継承]
10.辻 明彦
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[継承]
題名 (必須): (英) Subtilisin-like proprotein convertase paired basic amino acid-cleaving enzyme 4 is required for chondrogenic differentiation in ATDC5 cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Bone morphogenetic proteins (BMPs) have been implicated in the regulation of multiple stages of endochondral bone development. BMPs are synthesized as inactive precursors, and activated by removal of the propeptide. The subtilisin-like proprotein convertase (SPC) family comprises seven members [furin/SPC1, PC2/SPC2, PC1/PC3/SPC3, paired basic amino acid-cleaving enzyme 4 (PACE4)/SPC4, PC4/SPC5, PC6/PC5/SPC6, and PC8/PC7/LPC/SPC7], and activates various signaling molecules, including BMPs. In this study, we analyzed the role of this family in chondrogenic differentiation by using the mouse embryonal carcinoma-derived clonal cell line ATDC5. Both SPC-specific inhibitors, decanoyl-Arg-Val-Lys-Arg-chloromethylketone and α1-antitrypsin Portland variant, suppressed chondrogenic differentiation. RT-PCR analysis revealed that PACE4 mRNA levels increased markedly during chondrogenic differentiation, whereas furin expression remained unchanged. Knockdown of PACE4 expression significantly reduced chondrogenic differentiation. Furthermore, proBMP6, which shows an expression pattern similar to that of PACE4, was efficiently processed into its mature form by PACE4, whereas furin could not process proBMP6. These results suggest that PACE4 may regulate the rate of hypertrophic conversion of ATDC5 cells through activation of proBMP6.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acids, Basic (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Bone Morphogenetic Protein 6 (日) (読) [継承]
5. (英) Carcinoma, Embryonal (日) (読) [継承]
6. (英) Cell Differentiation (日) (読) [継承]
7. (英) Chondrocytes (日) (読) [継承]
8. (英) Furin (日) (読) [継承]
9. (英) Humans (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Proprotein Convertases (日) (読) [継承]
12. (英) RNA, Messenger (日) (読) [継承]
13. (英) RNA, Small Interfering (日) (読) [継承]
14. (英) Rats (日) (読) [継承]
15. (英) Real-Time Polymerase Chain Reaction (日) (読) [継承]
16. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
17. (英) Tumor Cells, Cultured (日) (読) [継承]
18. (英) alpha 1-Antitrypsin (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The FEBS Journal ([Federation of European Biochemical Societies])
(pISSN: 1742-464X, eISSN: 1742-4658)

ISSN (任意): 1742-4658
ISSN: 1742-464X (pISSN: 1742-464X, eISSN: 1742-4658)
Title: The FEBS journal
Title(ISO): FEBS J.
Supplier: Federation of European Biochemical Societies
Publisher: Wiley Publishing
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 279 [継承]
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(必須): 3997 4009 [継承]
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年月日 (必須): 西暦 2012年 11月 初日 (平成 24年 11月 初日) [継承]
URL (任意):
DOI (任意): 10.1111/j.1742-4658.2012.08758.x    (→Scopusで検索) [継承]
PMID (任意): 22925071    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1111/j.1742-4658.2012.08758.x  (日)    [継承]
2.(英) Article.Affiliation: Department of Biological Science and Technology, University of Tokushima Graduate School, Japan.  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Keizo Yuasa, Go Futamatsu, Tsuyoshi Kawano, Masaki Muroshita, Yoko Kageyama, Hiromi Taichi, Hiroshi Ishikawa, Masami Nagahama, Yoshiko Matsuda and Akihiko Tsuji : Subtilisin-like proprotein convertase paired basic amino acid-cleaving enzyme 4 is required for chondrogenic differentiation in ATDC5 cells, The FEBS Journal, Vol.279, No.21, 3997-4009, 2012.
欧文冊子 ● Keizo Yuasa, Go Futamatsu, Tsuyoshi Kawano, Masaki Muroshita, Yoko Kageyama, Hiromi Taichi, Hiroshi Ishikawa, Masami Nagahama, Yoshiko Matsuda and Akihiko Tsuji : Subtilisin-like proprotein convertase paired basic amino acid-cleaving enzyme 4 is required for chondrogenic differentiation in ATDC5 cells, The FEBS Journal, Vol.279, No.21, 3997-4009, 2012.

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