○種別 (必須): | □ | 学術論文 (審査論文)
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○言語 (必須): | □ | 英語
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○学究種別 (推奨): |
○組織 (推奨): |
○著者 (必須): | 1. | (英) Abe Hiroshi (日) (読)
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| 2. | (英) Wang Jin (日) (読)
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| 3. | 古川 和寛
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| 4. | (英) Oki Kazuma (日) (読)
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| 5. | (英) Uda Miwako (日) (読)
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| 6. | (英) Tsuneda Satoshi (日) (読)
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| 7. | (英) Ito Yoshihiro (日) (読)
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○題名 (必須): | □ | (英) A reduction-triggered fluorescence probe for sensing nucleic acids. (日)
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○要約 (任意): | □ | (英) We have developed a reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from Rhodamine for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and the reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The signal/background ratio of this fluorogenic compound reached 2100-fold enhancement in fluorescence intensity. Dithio-1,4-threitol or triphenylphosphine as reducing reagents were successfully utilized for this chemistry to be introduced into the DNA probe. The genetic detection requires that two strands of DNA bind onto target oligonucleotides, one probe carrying a reducible fluorogenic compound while the other carries the reducing reagents. The reaction proceeds automatically without any enzymes or reagents under biological conditions to produce a fluorescence signal within 10-20 min in the presence of target DNA or RNA. In addition, the probe was very stable under biological conditions, even such extreme conditions as pH 5 solution, pH 10 solution, or high temperature (90 degrees C) with no undesirable background signal. The probes were successfully applied to the detection of oligonucleotides at the single nucleotide level in solution and endogenous RNA in bacterial cells. (日)
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○キーワード (推奨): | 1. | (英) Azides (日) (読)
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| 2. | (英) Escherichia coli K12 (日) (読)
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| 3. | (英) Fluorescence (日) (読)
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| 4. | (英) Fluorescent Dyes (日) (読)
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| 5. | (英) Humans (日) (読)
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| 6. | (英) Indicators and Reagents (日) (読)
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| 7. | (英) Nucleic Acids (日) (読)
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| 8. | (英) Oligonucleotides (日) (読)
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| 9. | 酸化と還元 (oxidation and reduction)
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| 10. | (英) RNA, Ribosomal, 23S (日) (読)
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| 11. | (英) Rhodamines (日) (読)
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○発行所 (推奨): |
○誌名 (必須): | □ | Bioconjugate Chemistry ([アメリカ化学会])
(pISSN: 1043-1802, eISSN: 1520-4812)
○ISSN (任意): | □ | 1520-4812
ISSN: 1043-1802
(pISSN: 1043-1802, eISSN: 1520-4812) Title: Bioconjugate chemistryTitle(ISO): Bioconjug ChemPublisher: American Chemical Society (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login])
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○巻 (必須): | □ | 19
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○号 (必須): | □ | 6
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○頁 (必須): | □ | 1219 1226
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○都市 (任意): |
○年月日 (必須): | □ | 西暦 2008年 5月 14日 (平成 20年 5月 14日)
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○URL (任意): |
○DOI (任意): | □ | 10.1021/bc800014d (→Scopusで検索)
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○PMID (任意): | □ | 18476727 (→Scopusで検索)
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○CRID (任意): |
○WOS (任意): | □ | 000256950700016
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○Scopus (任意): | □ | 2-s2.0-45749140131
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○備考 (任意): | 1. | (英) Affiliation: Nano Medical Engineering Laboratory, Discovery Research Institute, RIKEN, 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 Japan. h-abe@riken.jp (日)
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| 2. | (英) PublicationType: Journal Article (日)
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| 3. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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