○種別 (必須): | □ | 学術論文 (審査論文)
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○言語 (必須): | □ | 英語
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○審査 (推奨): |
○カテゴリ (推奨): |
○共著種別 (推奨): |
○学究種別 (推奨): |
○組織 (推奨): |
○著者 (必須): | 1. | (英) Abe Naoko (日) (読)
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| 2. | (英) Abe Hiroshi (日) (読)
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| 3. | (英) Nagai Chisato (日) (読)
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| 4. | (英) Harada Mitsuru (日) (読)
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| 5. | (英) Hatakeyama Hiroto (日) (読)
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| 6. | (英) Harashima Hideyoshi (日) (読)
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| 7. | (英) Ohshiro Takahito (日) (読)
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| 8. | (英) Nishihara Mizuki (日) (読)
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| 9. | 古川 和寛
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| 10. | (英) Maeda Mizuo (日) (読)
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| 11. | (英) Tsuneda Satoshi (日) (読)
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| 12. | (英) Ito Yoshihiro (日) (読)
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○題名 (必須): | □ | (英) Synthesis, structure, and biological activity of dumbbell-shaped nanocircular RNAs for RNA interference. (日)
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○副題 (任意): |
○要約 (任意): | □ | (英) RNA interference (RNAi) is one of the most promising new approaches for disease therapy. The design of a dumbbell-shaped nanocircular RNA allows it to act as a short interfering RNA (siRNA) precursor. To optimize the design, we studied the relationship between the nanostructure and RNAi activity by synthesizing various RNA dumbbells. An RNA dumbbell with a 23-bp stem and 9-nt loops was the most potent. Sequence analysis by mass spectrometry showed that Dicer could edit RNA dumbbells to siRNA species. The reaction offered the slow release of siRNA species, which conferred prolonged RNAi activity. Introduction of DNA into the loop position significantly stabilized the dumbbell in biological fluid without any loss of RNAi activity. In-depth pharmacological evaluation was performed by introducing dumbbells into HeLa cells that stably express the target luciferase gene. The dumbbells provided a rapid silencing effect and retained this effect for a longer time even at a lower concentration than that at which standard siRNA completely lost RNAi activity. We conclude that an RNA dumbbell with DNA loops is the most promising design for in vivo applications for RNA medicine. (日)
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○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Base Sequence (日) (読)
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| 3. | (英) Cell Line (日) (読)
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| 4. | (英) HeLa Cells (日) (読)
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| 5. | (英) Humans (日) (読)
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| 6. | (英) Mice (日) (読)
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| 7. | (英) Molecular Sequence Data (日) (読)
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| 8. | (英) NIH 3T3 Cells (日) (読)
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| 9. | (英) Nanostructures (日) (読)
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| 10. | (英) Nucleic Acid Conformation (日) (読)
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| 11. | RNA (RNA)
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| 12. | (英) RNA Interference (日) (読)
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| 13. | (英) RNA Stability (日) (読)
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| 14. | (英) RNA, Small Interfering (日) (読)
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| 15. | (英) Ribonuclease III (日) (読)
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○発行所 (推奨): |
○誌名 (必須): | □ | Bioconjugate Chemistry ([アメリカ化学会])
(pISSN: 1043-1802, eISSN: 1520-4812)
○ISSN (任意): | □ | 1520-4812
ISSN: 1043-1802
(pISSN: 1043-1802, eISSN: 1520-4812) Title: Bioconjugate chemistryTitle(ISO): Bioconjug ChemPublisher: American Chemical Society (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login])
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○巻 (必須): | □ | 22
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○号 (必須): | □ | 10
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○頁 (必須): | □ | 2082 2092
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○年月日 (必須): | □ | 西暦 2011年 9月 23日 (平成 23年 9月 23日)
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○URL (任意): |
○DOI (任意): | □ | 10.1021/bc2003154 (→Scopusで検索)
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○PMID (任意): | □ | 21899349 (→Scopusで検索)
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○CRID (任意): |
○WOS (任意): | □ | 000295915100023
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○Scopus (任意): | □ | 2-s2.0-80054810308
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○備考 (任意): | 1. | (英) Affiliation: Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, Wako-shi, Saitama, Japan. h-abe@riken.jp (日)
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| 2. | (英) PublicationType: Journal Article (日)
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| 3. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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