『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1.宮脇 克行 ([徳島大学.バイオイノベーション研究所]/[徳島大学.生物資源産業学部.生物資源産業学科.生物生産システム講座])
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2. (英) Fukuoka Sachi (日) (読)
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3. (英) Kadomura Yasuko (日) (読)
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4. (英) Hamaoka Hirokazu (日) (読)
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5.三戸 太郎 ([徳島大学.バイオイノベーション研究所]/[徳島大学.生物資源産業学部.生物資源産業学科.生物生産システム講座])
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6.大内 淑代
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7. (英) Schwab Wilfried (日) (読)
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8.野地 澄晴 ([徳島大学])
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題名 (必須): (英) Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique  (日)    [継承]
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要約 (任意): (英) Traditional methods used to study strawberry ripening-related gene function are time-consuming, and require at least 15 months from initiating the transformation experiment until the first ripe fruits are available for analysis. To accelerate data acquisition during gene function studies, we explored a transient assay method that employs an Agrobacterium-mediated RNAi (AmRNAi) technique in post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar. Our results showed that artificial white light induced strong expression of Fa chalcone synthase (Fa CHS), Fa chalcone isomerase (Fa CHI), and Fa flavonoid 3 -hydroxylase orthologues (Fa F3 H) in post-harvest fruit. Fa CHS and Fa F3 H function was subsequently examined by performing AmRNAi with post-harvest fruit. Although reduction of light-induced Fa F3 H expression by AmRNAi resulted in no significant change in anthocyanin content, reduction of Fa CHS significantly decreased anthocyanin levels, and up-regulated Fa F3 H levels. Our results are consistent with previous data indicating that while CHS is required for anthocyanin accumulation during late stage strawberry fruit maturation, Fa F3 H is not required. The novel system described here enabled gene function data to be available within 10 days of initiating the incubation period following infiltration. Therefore, we conclude our system is a valuable tool to elucidate the molecular mechanisms underlying light-induced ripening of strawberry fruit.  (日)    [継承]
キーワード (推奨): 1. (英) Agrobacterium-mediated RNAi (日) (読) [継承]
2. (英) chalcone synthase (日) (読) [継承]
3. (英) flavonoid 3'-hydroxylase (日) (読) [継承]
4. (英) light-induced pigmentation (日) (読) [継承]
5. (英) strawberry fruit (日) (読) [継承]
発行所 (推奨): (英) Japanese Society for Plant Cell and Molecular Biology (日) 日本植物細胞分子生物学会 (読) [継承]
誌名 (必須): Plant Biotechnology (日本植物細胞分子生物学会)
(pISSN: 1342-4580, eISSN: 1347-6114)

ISSN (任意): 1342-4580
ISSN: 1342-4580 (pISSN: 1342-4580, eISSN: 1347-6114)
Title: Plant biotechnology (Tokyo, Japan)
Title(ISO): Plant Biotechnol (Tokyo)
Supplier: 日本植物バイオテクノロジー学会
Publisher: Japanese Society of Plant Cell and Molecular Biology
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年月日 (必須): 西暦 2012年 6月 25日 (平成 24年 6月 25日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/10030804744/ [継承]
DOI (任意): 10.5511/plantbiotechnology.12.0406a    (→Scopusで検索) [継承]
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NAID (任意): 10030804744 [継承]
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標準的な表示

和文冊子 ● Katsuyuki Miyawaki, Sachi Fukuoka, Yasuko Kadomura, Hirokazu Hamaoka, Taro Mito, Hideyo Ohuchi, Wilfried Schwab and Sumihare Noji : Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique, Plant Biotechnology, Vol.29, 271-278, 2012.
欧文冊子 ● Katsuyuki Miyawaki, Sachi Fukuoka, Yasuko Kadomura, Hirokazu Hamaoka, Taro Mito, Hideyo Ohuchi, Wilfried Schwab and Sumihare Noji : Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique, Plant Biotechnology, Vol.29, 271-278, 2012.

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