『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=254564EID:254564, Map:0, LastModified:2012年8月29日(水) 18:03:43, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[堤 理恵], Read:継承, Write:継承, Delete:継承.
種別 (必須):
言語 (必須): 英語 [継承]
招待 (推奨):
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カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Mistry S. Devendra (日) (読)
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学籍番号 (推奨):
[継承]
2.堤 理恵 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.代謝栄養学])
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学籍番号 (推奨):
[継承]
3. (英) Fernandez Marina (日) (読)
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[継承]
4. (英) Sharma Shweta (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Cardenas A. Steven (日) (読)
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学籍番号 (推奨):
[継承]
6. (英) Lawson A. Mark (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Webster J. G. Nicholas (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Gonadotropin-releasing hormone pulse sensitivity of follicle-stimulating hormone-beta gene is mediated by differential expression of positive regulatory activator protein 1 factors and corepressors SKIL and TGIF1.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Gonadotropin synthesis and release is dependent on pulsatile stimulation by the hypothalamic neuropeptide GnRH. Generally, slow GnRH pulses promote FSH production, whereas rapid pulses favor LH, but the molecular mechanism underlying this pulse sensitivity is poorly understood. In this study, we developed and tested a model for FSHβ regulation in mouse LβT2 gonadotropes. By mining a previous microarray data set, we found that mRNA for positive regulators of Fshb expression, such as Fos and Jun, were up-regulated at slower pulse frequencies than a number of potential negative regulators, such as the corepressors Skil, Crem, and Tgif1. These latter corepressors reduced Fshb promoter activity whether driven by transfection of individual transcription factors or by treatment with GnRH and activin. Overexpression of binding or phosphorylation-defective ski-oncogene-like protein (SKIL) and TG interacting factor (TGIF1) mutants, however, failed to repress Fshb promoter activity. Knockdown of the endogenous repressors SKIL and TGIF1, but not cAMP response element-modulator, increased Fshb promoter activity driven by constant GnRH or activin. Chromatin immunoprecipitation analysis showed that FOS, SKIL, and TGIF1 occupy the FSHβ promoter in a cyclical manner after GnRH stimulation. Overexpression of corepressors SKIL or TGIF1 repressed induction of the Fshb promoter at the slow GnRH pulse frequency but had little effect at the fast pulse frequency. In contrast, knockdown of endogenous SKIL or TGIF1 selectively increased Fshb mRNA at the fast GnRH pulse frequency. Therefore, we propose a potential mechanism by which production of gonadotropin Fshb is modulated by positive transcription factors and negative corepressors with different pulse sensitivities.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Co-Repressor Proteins (日) (読) [継承]
3. (英) Cyclic AMP Response Element Modulator (日) (読) [継承]
4. (英) Follicle Stimulating Hormone, beta Subunit (日) (読) [継承]
5. (英) Gene Expression Regulation (日) (読) [継承]
6. (英) Gene Knockdown Techniques (日) (読) [継承]
7. (英) Gonadotropin-Releasing Hormone (日) (読) [継承]
8. (英) Homeodomain Proteins (日) (読) [継承]
9. (英) Kinetics (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Models, Biological (日) (読) [継承]
12. (英) Mutation (日) (読) [継承]
13. (英) Promoter Regions, Genetic (日) (読) [継承]
14. (英) Protein Subunits (日) (読) [継承]
15. (英) Proto-Oncogene Proteins (日) (読) [継承]
16. (英) Proto-Oncogene Proteins c-fos (日) (読) [継承]
17. (英) Proto-Oncogene Proteins c-jun (日) (読) [継承]
18. (英) RNA, Messenger (日) (読) [継承]
19. (英) Rats (日) (読) [継承]
20. (英) Repressor Proteins (日) (読) [継承]
21. (英) Transcription Factor AP-1 (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular Endocrinology ([The Endocrine Society])
(pISSN: 0888-8809, eISSN: 1944-9917)

ISSN (任意): 1944-9917
ISSN: 0888-8809 (pISSN: 0888-8809, eISSN: 1944-9917)
Title: Molecular endocrinology (Baltimore, Md.)
Title(ISO): Mol Endocrinol
Publisher: Endocrine Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 25 [継承]
(必須): 8 [継承]
(必須): 1387 1403 [継承]
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年月日 (必須): 西暦 2011年 6月 9日 (平成 23年 6月 9日) [継承]
URL (任意):
DOI (任意): 10.1210/me.2011-0032    (→Scopusで検索) [継承]
PMID (任意): 21659477    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Medical Research Service, Veterans Affairs San Diego Healthcare System, San Diego, California 92161, USA.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural  (日)    [継承]
4.(英) OtherID: PMC3146254  (日)    [継承]

標準的な表示

和文冊子 ● (種別) : Devendra S. Mistry, Rie Tsutsumi, Marina Fernandez, Shweta Sharma, Steven A. Cardenas, Mark A. Lawson and Nicholas J. G. Webster : Gonadotropin-releasing hormone pulse sensitivity of follicle-stimulating hormone-beta gene is mediated by differential expression of positive regulatory activator protein 1 factors and corepressors SKIL and TGIF1., Molecular Endocrinology, Vol.25, No.8, 1387-1403, (発行所), (都市), June 2011.
欧文冊子 ● (種別) : Devendra S. Mistry, Rie Tsutsumi, Marina Fernandez, Shweta Sharma, Steven A. Cardenas, Mark A. Lawson and Nicholas J. G. Webster : Gonadotropin-releasing hormone pulse sensitivity of follicle-stimulating hormone-beta gene is mediated by differential expression of positive regulatory activator protein 1 factors and corepressors SKIL and TGIF1., Molecular Endocrinology, Vol.25, No.8, 1387-1403, (発行所), (都市), June 2011.

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