『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (紀要その他) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Liu Zun (日) (読)
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学籍番号 (推奨):
[継承]
2.瀬川 博子 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.応用栄養学])
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学籍番号 (推奨):
[継承]
3. (英) Aydin Cumhur (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Reyes Monica (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Erben Reinhold G (日) (読)
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学籍番号 (推奨):
[継承]
6. (英) Weinstein Lee S (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Chen Min (日) (読)
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学籍番号 (推奨):
[継承]
8. (英) Marshansky Vladimir (日) (読)
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[継承]
9. (英) Fröhlich Leopold F (日) (読)
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[継承]
10. (英) Bastepe Murat (日) (読)
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[継承]
題名 (必須): (英) Transgenic overexpression of the extra-large Gs variant XLs enhances Gs-mediated responses in the mouse renal proximal tubule in vivo.  (日)    [継承]
副題 (任意):
要約 (任意): (英) XLs, a variant of the stimulatory G protein -subunit (Gs), can mediate receptor-activated cAMP generation and, thus, mimic the actions of Gs in transfected cells. However, it remains unknown whether XLs can act in a similar manner in vivo. We have now generated mice with ectopic transgenic expression of rat XLs in the renal proximal tubule (rptXLs mice), where Gs mediates most actions of PTH. Western blots and quantitative RT-PCR showed that, while Gs and type-1 PTH receptor levels were unaltered, protein kinase A activity and 25-hydroxyvitamin D 1--hydroxylase (Cyp27b1) mRNA levels were significantly higher in renal proximal tubules of rptXLs mice than wild-type littermates. Immunohistochemical analysis of kidney sections showed that the sodium-phosphate cotransporter type 2a was modestly reduced in brush border membranes of male rptXLs mice compared to gender-matched controls. Serum calcium, phosphorus, and 1,25 dihydroxyvitamin D were within the normal range, but serum PTH was 30% lower in rptXLs mice than in controls (152 ± 16 vs. 222 ± 41 pg/ml; P < 0.05). After crossing the rptXLs mice to mice with ablation of maternal Gnas exon 1 (E1(m-/+)), male offspring carrying both the XLs transgene and maternal Gnas exon 1 ablation (rptXLs/E1(m-/+)) were significantly less hypocalcemic than gender-matched E1(m-/+) littermates. Both E1(m-/+) and rptXLs/E1(m-/+) offspring had higher serum PTH than wild-type littermates, but the degree of secondary hyperparathyroidism tended to be lower in rptXLs/E1(m-/+) mice. Hence, transgenic XLs expression in the proximal tubule enhanced Gs-mediated responses, indicating that XLs can mimic Gs in vivo.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Blotting, Northern (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Cell Line (日) (読) [継承]
5. (英) Female (日) (読) [継承]
6. (英) GTP-Binding Protein alpha Subunits, Gs (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8.免疫組織化学 (immunohistochemistry) [継承]
9. (英) Kidney Tubules, Proximal (日) (読) [継承]
10.ノックアウトマウス (knockout mice) [継承]
11. (英) Polymerase Chain Reaction (日) (読) [継承]
12. (英) Rats (日) (読) [継承]
13. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Endocrinology ([The Endocrine Society])
(pISSN: 0013-7227, eISSN: 1945-7170)

ISSN (任意): 1945-7170
ISSN: 0013-7227 (pISSN: 0013-7227, eISSN: 1945-7170)
Title: Endocrinology
Title(ISO): Endocrinology
Publisher: Endocrine Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 152 [継承]
(必須): 4 [継承]
(必須): 1222 1233 [継承]
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年月日 (必須): 西暦 2011年 2月 8日 (平成 23年 2月 8日) [継承]
URL (任意):
DOI (任意): 10.1210/en.2010-1034    (→Scopusで検索) [継承]
PMID (任意): 21303955    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Affiliation: Endocrine Unit, Department of Medicine, Massachusetts General Hospital, 50 Blossom Street, Thier 10, Boston, Massachusetts 02114, USA.  (日)    [継承]
2.(英) PublicationType: Journal Article  (日)    [継承]
3.(英) PublicationType: Research Support, N.I.H., Extramural  (日)    [継承]
4.(英) PublicationType: Research Support, N.I.H., Intramural  (日)    [継承]

標準的な表示

和文冊子 ● Zun Liu, Hiroko Segawa, Cumhur Aydin, Monica Reyes, G Reinhold Erben, S Lee Weinstein, Min Chen, Vladimir Marshansky, F Leopold Fröhlich and Murat Bastepe : Transgenic overexpression of the extra-large Gs variant XLs enhances Gs-mediated responses in the mouse renal proximal tubule in vivo., Endocrinology, Vol.152, No.4, 1222-1233, 2011.
欧文冊子 ● Zun Liu, Hiroko Segawa, Cumhur Aydin, Monica Reyes, G Reinhold Erben, S Lee Weinstein, Min Chen, Vladimir Marshansky, F Leopold Fröhlich and Murat Bastepe : Transgenic overexpression of the extra-large Gs variant XLs enhances Gs-mediated responses in the mouse renal proximal tubule in vivo., Endocrinology, Vol.152, No.4, 1222-1233, 2011.

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