『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Magari Masaki (日) (読)
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学籍番号 (推奨):
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2.西川 裕美子
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学籍番号 (推奨):
[継承]
3. (英) Fujii Yasumasa (日) (読)
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[継承]
4. (英) Nishio Yumi (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Watanabe Koji (日) (読)
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6. (英) Fujiwara Michiya (日) (読)
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7. (英) Kanayama Naoki (日) (読)
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[継承]
8. (英) Ohmori Hitoshi (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) In germinal centers (GCs), B cells are selected through interaction with follicular dendritic cells bearing immune complexes and follicular helper T (Tfh) cells secreting Tfh cytokines, including IL-21. To analyze these cellular interactions, we have explored culture conditions that can simulate GC B cell selection in vitro using a mouse follicular dendritic cell line, FL-YB. FL-YB cells efficiently enhanced viability of cocultured mouse B cells in a BAFF-dependent fashion. Interestingly, we found that addition of IL-21, a major Tfh cytokine, readily induced death of B cells that were cocultured with FL-YB cells, whereas IL-21 alone sustained viability of B cells in the absence of FL-YB cells. The IL-21-induced death was dependent on a low m.w. soluble factor that was released from FL-YB cells, which was finally identified as PGE(2). Treatment of B cells with IL-21 plus PGE(2), but not either alone, resulted in enhanced expression of a proapoptotic protein Bim and the upstream transcription factor Foxo1. A PGE(2) receptor isoform, EP4, was responsible for IL-21/PGE(2)-induced B cell death. Thus, PGE(2) is an endogenous chemical mediator that can switch pleiotropic actions of IL-21 on B cells. IL-21/PGE(2)-induced B cell death was rescued if B cells were costimulated via CD40. In immunized mice, deficiency of IL-21R in B cells led to a significant decrease in the frequency of activated caspase-3-positive GC B cells concomitant with impaired affinity maturation of Abs. Taken together, results implicate a physiological role of IL-21/PGE(2)-induced B cell death in GC B cell selection.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2.アポトーシス (apoptosis) [継承]
3. (英) B-Lymphocytes (日) (読) [継承]
4.細胞分化 (cell differentiation) [継承]
5. (英) Cell Line (日) (読) [継承]
6.細胞分離 (cell separation) [継承]
7. (英) Coculture Techniques (日) (読) [継承]
8. (英) Dendritic Cells, Follicular (日) (読) [継承]
9. (英) Dinoprostone (日) (読) [継承]
10. (英) Flow Cytometry (日) (読) [継承]
11. (英) Germinal Center (日) (読) [継承]
12. (英) Interleukins (日) (読) [継承]
13. (英) Lymphocyte Activation (日) (読) [継承]
14. (英) Male (日) (読) [継承]
15. (英) Mice (日) (読) [継承]
16. (英) Mice, Inbred BALB C (日) (読) [継承]
17. (英) Mice, Inbred C57BL (日) (読) [継承]
18. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN (任意): 1550-6606
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 187 [継承]
(必須): 8 [継承]
(必須): 4210 4218 [継承]
都市 (任意):
年月日 (必須): 西暦 2011年 9月 12日 (平成 23年 9月 12日) [継承]
URL (任意):
DOI (任意): 10.4049/jimmunol.1100934    (→Scopusで検索) [継承]
PMID (任意): 21911600    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Kita-ku, Okayama 700-8530, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Masaki Magari, Yumiko Nishikawa, Yasumasa Fujii, Yumi Nishio, Koji Watanabe, Michiya Fujiwara, Naoki Kanayama and Hitoshi Ohmori : IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells., The Journal of Immunology, Vol.187, No.8, 4210-4218, 2011.
欧文冊子 ● Masaki Magari, Yumiko Nishikawa, Yasumasa Fujii, Yumi Nishio, Koji Watanabe, Michiya Fujiwara, Naoki Kanayama and Hitoshi Ohmori : IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells., The Journal of Immunology, Vol.187, No.8, 4210-4218, 2011.

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