『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=251966EID:251966, Map:0, LastModified:2014年8月20日(水) 12:59:29, Operator:[谷口 寿章], Avail:TRUE, Censor:0, Owner:[谷口 寿章], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨):
著者 (必須): 1. (英) Iwakawa Hiro-Oki (日) (読)
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2. (英) Tajima Yuri (日) (読)
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3.谷口 貴子
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4. (英) Kaido Masanori (日) (読)
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5. (英) Mise Kazuyuki (日) (読)
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6. (英) Tomari Yukihide (日) (読)
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7.谷口 寿章
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8. (英) Okuno Tetsuro (日) (読)
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題名 (必須): (英) Poly(A)-binding protein facilitates translation of an uncapped/nonpolyadenylated viral RNA by binding to the 3' untranslated region.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Viruses employ an alternative translation mechanism to exploit cellular resources at the expense of host mRNAs and to allow preferential translation. Plant RNA viruses often lack both a 5' cap and a 3' poly(A) tail in their genomic RNAs. Instead, cap-independent translation enhancer elements (CITEs) located in the 3' untranslated region (UTR) mediate their translation. Although eukaryotic translation initiation factors (eIFs) or ribosomes have been shown to bind to the 3'CITEs, our knowledge is still limited for the mechanism, especially for cellular factors. Here, we searched for cellular factors that stimulate the 3'CITE-mediated translation of Red clover necrotic mosaic virus (RCNMV) RNA1 using RNA aptamer-based one-step affinity chromatography, followed by mass spectrometry analysis. We identified the poly(A)-binding protein (PABP) as one of the key players in the 3'CITE-mediated translation of RCNMV RNA1. We found that PABP binds to an A-rich sequence (ARS) in the viral 3' UTR. The ARS is conserved among dianthoviruses. Mutagenesis and a tethering assay revealed that the PABP-ARS interaction stimulates 3'CITE-mediated translation of RCNMV RNA1. We also found that both the ARS and 3'CITE are important for the recruitment of the plant eIF4F and eIFiso4F factors to the 3' UTR and of the 40S ribosomal subunit to the viral mRNA. Our results suggest that dianthoviruses have evolved the ARS and 3'CITE as substitutes for the 3' poly(A) tail and the 5' cap of eukaryotic mRNAs for the efficient recruitment of eIFs, PABP, and ribosomes to the uncapped/nonpolyadenylated viral mRNA.  (日)    [継承]
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誌名 (必須): Journal of Virology ([アメリカ微生物学会])
(pISSN: 0022-538X, eISSN: 1098-5514)

ISSN (任意): 1098-5514
ISSN: 0022-538X (pISSN: 0022-538X, eISSN: 1098-5514)
Title: Journal of virology
Title(ISO): J Virol
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 86 [継承]
(必須): 15 [継承]
(必須): 7836 7849 [継承]
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年月日 (必須): 西暦 2012年 5月 16日 (平成 24年 5月 16日) [継承]
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DOI (任意): 10.1128/JVI.00538-12    (→Scopusで検索) [継承]
PMID (任意): 22593149    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Hiro-Oki Iwakawa, Yuri Tajima, Takako Taniguchi, Masanori Kaido, Kazuyuki Mise, Yukihide Tomari, Hisaaki Taniguchi and Tetsuro Okuno : Poly(A)-binding protein facilitates translation of an uncapped/nonpolyadenylated viral RNA by binding to the 3' untranslated region., Journal of Virology, Vol.86, No.15, 7836-7849, 2012.
欧文冊子 ● Hiro-Oki Iwakawa, Yuri Tajima, Takako Taniguchi, Masanori Kaido, Kazuyuki Mise, Yukihide Tomari, Hisaaki Taniguchi and Tetsuro Okuno : Poly(A)-binding protein facilitates translation of an uncapped/nonpolyadenylated viral RNA by binding to the 3' untranslated region., Journal of Virology, Vol.86, No.15, 7836-7849, 2012.

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