『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=250959EID:250959, Map:0, LastModified:2016年8月9日(火) 17:59:55, Operator:[中西 正], Avail:TRUE, Censor:0, Owner:[中西 正], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Franco C. N. Gilson (日) (読)
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[継承]
2. (英) Kajiya Mikihito (日) (読)
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[継承]
3.中西 正 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯科保存学]/[徳島大学.病院.診療科.歯科.むし歯科(第一保存科)])
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学籍番号 (推奨):
[継承]
4. (英) Ohta Kouji (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Rosalen L. Pedro (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Groppo C. Francisco (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Ernst W. O. Cory (日) (読)
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学籍番号 (推奨):
[継承]
8. (英) Boyesen L. Janie (日) (読)
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学籍番号 (推奨):
[継承]
9. (英) Bartlett D. John (日) (読)
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学籍番号 (推奨):
[継承]
10. (英) Stashenko Philip (日) (読)
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[継承]
11. (英) Taubman A. Martin (日) (読)
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学籍番号 (推奨):
[継承]
12. (英) Kawai Toshihisa (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Inhibition of matrix metalloproteinase-9 activity by doxycycline ameliorates RANK ligand-induced osteoclast differentiation in vitro and in vivo.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Tetracycline antibiotics, including doxycycli (DOX), have been used to treat bone resorptive diseases, partially because of their activity to suppress osteoclastogenesis induced by receptor activator of nuclear factor kappa B ligand (RANKL). However, their precise inhibitory mechanism remains unclear. Therefore, the present study examined the effect of Dox on osteoclastogenesis signaling induced by RANKL, both in vitro and in vivo. Although Dox inhibited RANKL-induced osteoclastogenesis and down-modulated the mRNA expression of functional osteoclast markers, including tartrate-resistant acid phosphatase (TRAP) and cathepsin K, Dox neither affected RANKL-induced MAPKs phosphorylation nor NFATc1 gene expression in RAW264.7 murine monocytic cells. Gelatin zymography and Western blot analyses showed that Dox down-regulated the enzyme activity of RANKL-induced MMP-9, but without affecting its protein expression. Furthermore, MMP-9 enzyme inhibitor also attenuated both RANKL-induced osteoclastogenesis and up-regulation of TRAP and cathepsin K mRNA expression, indicating that MMP-9 enzyme action is engaged in the promotion of RANKL-induced osteoclastogenesis. Finally, Dox treatment abrogated RANKL-induced osteoclastogenesis and TRAP activity in mouse calvaria along with the suppression of MMP9 enzyme activity, again without affecting the expression of MMP9 protein. These findings suggested that Dox inhibits RANKL-induced osteoclastogenesis by its inhibitory effect on MMP-9 enzyme activity independent of the MAPK-NFATc1 signaling cascade.  (日)    [継承]
キーワード (推奨): 1. (英) Acid Phosphatase (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Anti-Bacterial Agents (日) (読) [継承]
4. (英) Blotting, Western (日) (読) [継承]
5. (英) Bone Resorption (日) (読) [継承]
6. (英) Bone and Bones (日) (読) [継承]
7. (英) Cathepsin K (日) (読) [継承]
8. (英) Cell Differentiation (日) (読) [継承]
9. (英) Cells, Cultured (日) (読) [継承]
10. (英) Doxycycline (日) (読) [継承]
11. (英) Isoenzymes (日) (読) [継承]
12. (英) Male (日) (読) [継承]
13. (英) Matrix Metalloproteinase 9 (日) (読) [継承]
14. (英) Mice (日) (読) [継承]
15. (英) Mice, Inbred BALB C (日) (読) [継承]
16. (英) NFATC Transcription Factors (日) (読) [継承]
17. (英) Osteoclasts (日) (読) [継承]
18. (英) Phosphorylation (日) (読) [継承]
19. (英) RANK Ligand (日) (読) [継承]
20. (英) RNA, Messenger (日) (読) [継承]
21. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
22. (英) Skull (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Experimental Cell Research ([Academic Press])
(pISSN: 0014-4827, eISSN: 1090-2422)

ISSN (任意): 1090-2422
ISSN: 0014-4827 (pISSN: 0014-4827, eISSN: 1090-2422)
Title: Experimental cell research
Title(ISO): Exp. Cell Res.
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 317 [継承]
(必須): 10 [継承]
(必須): 1454 1464 [継承]
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年月日 (必須): 西暦 2011年 3月 21日 (平成 23年 3月 21日) [継承]
URL (任意):
DOI (任意): 10.1016/j.yexcr.2011.03.014    (→Scopusで検索) [継承]
PMID (任意): 21420951    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Immunology, Forsyth Institute, Cambridge, MA 02142, USA.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: In Vitro  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural  (日)    [継承]
5.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
6.(英) OtherID: NIHMS291192  (日)    [継承]
7.(英) OtherID: PMC3115670  (日)    [継承]

標準的な表示

和文冊子 ● Gilson C. N. Franco, Mikihito Kajiya, Tadashi Nakanishi, Kouji Ohta, Pedro L. Rosalen, Francisco C. Groppo, Cory W. O. Ernst, Janie L. Boyesen, John D. Bartlett, Philip Stashenko, Martin A. Taubman and Toshihisa Kawai : Inhibition of matrix metalloproteinase-9 activity by doxycycline ameliorates RANK ligand-induced osteoclast differentiation in vitro and in vivo., Experimental Cell Research, Vol.317, No.10, 1454-1464, 2011.
欧文冊子 ● Gilson C. N. Franco, Mikihito Kajiya, Tadashi Nakanishi, Kouji Ohta, Pedro L. Rosalen, Francisco C. Groppo, Cory W. O. Ernst, Janie L. Boyesen, John D. Bartlett, Philip Stashenko, Martin A. Taubman and Toshihisa Kawai : Inhibition of matrix metalloproteinase-9 activity by doxycycline ameliorates RANK ligand-induced osteoclast differentiation in vitro and in vivo., Experimental Cell Research, Vol.317, No.10, 1454-1464, 2011.

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