○種別 (必須): | □ | 学術論文 (審査論文)
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○言語 (必須): | □ | 英語
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○共著種別 (推奨): |
○学究種別 (推奨): |
○組織 (推奨): |
○著者 (必須): | 1. | (英) Ueta Yoichi (日) (読)
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| 2. | 藤原 広明
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| 3. | (英) Serino Ryota (日) (読)
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| 4. | (英) Dayanithi Govindan (日) (読)
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| 5. | (英) Ozawa Hitoshi (日) (読)
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| 6. | (英) Matsuda Ken-ichi (日) (読)
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| 7. | (英) Kawata Mitsuhiro (日) (読)
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| 8. | (英) Yamada Junko (日) (読)
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| 9. | (英) Ueno Shinya (日) (読)
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| 10. | (英) Fukuda Atsuo (日) (読)
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| 11. | (英) Murphy David (日) (読)
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○題名 (必須): | □ | (英) Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons and isolated nerve terminals of the rat. (日)
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○副題 (任意): |
○要約 (任意): | □ | (英) We have generated transgenic rats expressing an arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene. The expression of the eGFP gene and strong fluorescence were observed in the supraoptic nucleus (SON), the paraventricular nucleus (PVN), and the suprachiasmatic nucleus (SCN) in transgenic rats. The hypothalamo-neurohypophyseal tract, isolated SON neurons, and isolated axon terminals in the neurohypophysis also showed robust eGFP fluorescence. Water deprivation for 2 d increased the fluorescence of the eGFP in the SON and the PVN but not the SCN. The whole-cell patch-clamp technique was then used to record the electrical activities specifically identifying eGFP-expressing SON, PVN, and SCN AVP neurons in in vitro brain slice preparations. The AVP-eGFP transgenic rats are a unique new tool with which to study the physiological role of AVP-secreting neurons in the central nervous system and the dynamics of the regulation of AVP secretion in the living neurons and their axon terminals. (日)
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○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Animals, Genetically Modified (日) (読)
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| 3. | (英) Arginine Vasopressin (日) (読)
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| 4. | 脳 (brain)
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| 5. | (英) Dehydration (日) (読)
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| 6. | 遺伝子発現 (gene expression)
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| 7. | (英) Green Fluorescent Proteins (日) (読)
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| 8. | 免疫組織化学 (immunohistochemistry)
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| 9. | (英) In Situ Hybridization (日) (読)
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| 10. | (英) Nerve Endings (日) (読)
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| 11. | (英) Neurons (日) (読)
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| 12. | (英) Oxytocin (日) (読)
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| 13. | (英) Patch-Clamp Techniques (日) (読)
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| 14. | (英) RNA, Messenger (日) (読)
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| 15. | (英) Rats (日) (読)
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| 16. | (英) Rats, Wistar (日) (読)
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| 17. | (英) Recombinant Fusion Proteins (日) (読)
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| 18. | (英) Tissue Distribution (日) (読)
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| 19. | (英) Water Deprivation (日) (読)
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○発行所 (推奨): |
○誌名 (必須): | □ | Endocrinology ([The Endocrine Society])
(pISSN: 0013-7227, eISSN: 1945-7170)
○ISSN (任意): | □ | 0013-7227
ISSN: 0013-7227
(pISSN: 0013-7227, eISSN: 1945-7170) Title: EndocrinologyTitle(ISO): EndocrinologyPublisher: Oxford University Press (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login])
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○巻 (必須): | □ | 146
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○号 (必須): | □ | 1
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○頁 (必須): | □ | 406 413
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○都市 (任意): |
○年月日 (必須): | □ | 西暦 2004年 9月 16日 (平成 16年 9月 16日)
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○URL (任意): |
○DOI (任意): | □ | 10.1210/en.2004-0830 (→Scopusで検索)
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○PMID (任意): | □ | 15375027 (→Scopusで検索)
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○CRID (任意): |
○WOS (任意): | □ | 000225766500054
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○Scopus (任意): | □ | 2-s2.0-19944396441
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○備考 (任意): | 1. | (英) Affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan. yoichi@med.uoeh-u.ac.jp (日)
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| 2. | (英) PublicationType: In Vitro (日)
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| 3. | (英) PublicationType: Journal Article (日)
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| 4. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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