『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=248741)

EID=248741EID:248741, Map:0, LastModified:2014年3月4日(火) 17:08:22, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[学科長]/[徳島大学.医学部.医学科]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.藤原 広明
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Ueta Yoichi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Suzuki Hitoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Katoh Akiko (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Ohbuchi Toyoaki (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Otsubo Hiroki (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Dayanithi Govindan (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Murphy David (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Robust up-regulation of nuclear red fluorescent-tagged fos marks neuronal activation in green fluorescent vasopressin neurons after osmotic stimulation in a double-transgenic rat.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The up-regulation in the expression of mRNA or protein encoded by the c-fos gene is widely used as a marker of neuronal activation elicited by various stimuli. To facilitate the detection of activated neurons, we generated transgenic rats expressing a fusion gene consisting of c-fos coding sequences in frame with monomeric red fluorescent protein 1 (mRFP1) under the control of c-fos gene regulatory sequences (c-fos-mRFP1 rats). In c-fos-mRFP1 transgenic rats, 90 min after hypertonic saline ip administration, nuclear mRFP1 fluorescence was observed abundantly in brain regions known to be osmosensitive, namely the median preoptic nucleus, organum vasculosum lamina terminalis, supraoptic nucleus, paraventricular nucleus, and subfornical organ. Immunohistochemistry for Fos protein confirmed that the distribution of Fos-like immunoreactivity in nontransgenic rats was similar to those of mRFP1 fluorescence after ip administration of hypertonic saline in the transgenic rats. Several double-transgenic rats were obtained from matings between transgenic rats expressing an arginine vasopressin-enhanced green fluorescent protein fusion gene (AVP-eGFP rats) and c-fos-mRFP1 rats. In these double-transgenic rats, almost all eGFP neurons in the supraoptic nucleus and PVN expressed nuclear mRFP1 fluorescence 90 min after hypertonic saline administration. The c-fos-mRFP1 rats are a powerful tool that enables the facile identification of activated neurons in the nervous system. Furthermore, when combined with transgenes expressing another fluorophore under the control of cell-specific regulatory sequences, activation of specific neuronal cell types in response to physiological cues can be readily detected.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Arginine Vasopressin (日) (読) [継承]
3. (brain) [継承]
4. (英) Female (日) (読) [継承]
5. (英) Green Fluorescent Proteins (日) (読) [継承]
6.視床下部 (hypothalamus) [継承]
7.免疫組織化学 (immunohistochemistry) [継承]
8. (英) Luminescent Proteins (日) (読) [継承]
9. (英) Male (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Mice, Inbred C57BL (日) (読) [継承]
12. (英) Microscopy, Confocal (日) (読) [継承]
13. (英) Microscopy, Fluorescence (日) (読) [継承]
14. (英) Neurons (日) (読) [継承]
15. (英) Paraventricular Hypothalamic Nucleus (日) (読) [継承]
16. (英) Proto-Oncogene Proteins c-fos (日) (読) [継承]
17. (英) Rats (日) (読) [継承]
18. (英) Rats, Transgenic (日) (読) [継承]
19. (英) Rats, Wistar (日) (読) [継承]
20. (英) Recombinant Fusion Proteins (日) (読) [継承]
21. (英) Regulatory Sequences, Nucleic Acid (日) (読) [継承]
22. (英) Saline Solution, Hypertonic (日) (読) [継承]
23. (英) Subfornical Organ (日) (読) [継承]
24. (英) Supraoptic Nucleus (日) (読) [継承]
25. (英) Up-Regulation (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Endocrinology ([The Endocrine Society])
(pISSN: 0013-7227, eISSN: 1945-7170)

ISSN (任意): 1945-7170
ISSN: 0013-7227 (pISSN: 0013-7227, eISSN: 1945-7170)
Title: Endocrinology
Title(ISO): Endocrinology
Publisher: Endocrine Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 150 [継承]
(必須): 12 [継承]
(必須): 5633 5638 [継承]
都市 (任意):
年月日 (必須): 西暦 2009年 10月 22日 (平成 21年 10月 22日) [継承]
URL (任意):
DOI (任意): 10.1210/en.2009-0796    (→Scopusで検索) [継承]
PMID (任意): 19850746    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000272042300048 [継承]
Scopus (任意): 2-s2.0-71949097426 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hiroaki Fujihara, Yoichi Ueta, Hitoshi Suzuki, Akiko Katoh, Toyoaki Ohbuchi, Hiroki Otsubo, Govindan Dayanithi and David Murphy : Robust up-regulation of nuclear red fluorescent-tagged fos marks neuronal activation in green fluorescent vasopressin neurons after osmotic stimulation in a double-transgenic rat., Endocrinology, Vol.150, No.12, 5633-5638, 2009.
欧文冊子 ● Hiroaki Fujihara, Yoichi Ueta, Hitoshi Suzuki, Akiko Katoh, Toyoaki Ohbuchi, Hiroki Otsubo, Govindan Dayanithi and David Murphy : Robust up-regulation of nuclear red fluorescent-tagged fos marks neuronal activation in green fluorescent vasopressin neurons after osmotic stimulation in a double-transgenic rat., Endocrinology, Vol.150, No.12, 5633-5638, 2009.

関連情報

Number of session users = 0, LA = 0.40, Max(EID) = 383294, Max(EOID) = 1024468.