| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): | □ | 研究
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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| 2. | (英) Kim Wook-Dong (日) (読)
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| 3. | (英) Ozaki H. (日) (読)
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| 4. | (英) Kawaguchi Akira (日) (読)
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| 5. | (英) Honda Kohsuke (日) (読)
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| 6. | (英) Kajiura H. (日) (読)
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| 7. | (英) Fujiyama K. (日) (読)
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| 8. | (英) Asano Ryutaro (日) (読)
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| 9. | (英) Kumagai Izumi (日) (読)
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| 10. | (英) Ohtake Hisao (日) (読)
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| 11. | 大政 健史
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| ○題名 (必須): | □ | (英) Enhancement of sialylation on humanized lgG-like bispecific antibody by overexpression of 2,6-sialyltransferase derived from Chainese hamster ovary cells (日)
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| ○要約 (任意): | □ | (英) Improvement of glycosylation is one of the most important topics in the industrial production of therapeutic antibodies. We have focused on terminal sialylation with alpha-2,6 linkage, which is crucial for anti-inflammatory activity. In the present study, we have successfully cloned cDNA of beta-galactosyl alpha-2,6 sialyltransferase (ST6Gal I) derived from Chinese hamster ovary (CHO) cells regardless of reports that stated this was not endogenously expressed in CHO cells. After expressing cloned ST6Gal I in Escherichia coli, the transferase activity was confirmed by HPLC and lectin binding assay. Then, we applied ST6Gal I to alpha-2,6 sialylation of the recombinant antibody; the ST6Gal I expression vector was transfected into the CHO cell line producing a bispecific antibody. The N-glycosylation pattern of the antibody was estimated by HPLC and sialidase digestion. About 70% of the total N-linked oligosaccharide was alpha-2,6 sialylated in the transfected cell line whereas no sialylation was observed in the non-transfected cell line. The improvement of sialylation would be of practical importance for the industrial production of therapeutic antibodies. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Antibodies, Bispecific (日) (読)
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| 3. | (英) Antibodies, Monoclonal, Humanized (日) (読)
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| 4. | (英) CHO Cells (日) (読)
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| 5. | (英) Cricetinae (日) (読)
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| 6. | (英) Escherichia coli (日) (読)
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| 7. | (英) Female (日) (読)
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| 8. | (英) Gene Expression (日) (読)
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| 9. | (英) Humans (日) (読)
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| 10. | (英) Immunoglobulin G (日) (読)
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| 11. | (英) Ovary (日) (読)
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| 12. | (英) Recombinant Proteins (日) (読)
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| 13. | (英) Sialyltransferases (日) (読)
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| 14. | (英) Up-Regulation (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Applied Microbiology and Biotechnology ([Springer-Verlag])
(pISSN: 0175-7598, eISSN: 1432-0614)
| ○ISSN (任意): | □ | 1432-0614
ISSN: 0175-7598
(pISSN: 0175-7598, eISSN: 1432-0614) Title: Applied microbiology and biotechnologyTitle(ISO): Appl Microbiol BiotechnolSupplier: Springer Online Journal ArchivePublisher: Springer Berlin Heidelberg (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 94
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 69 80
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2012年 4月 初日 (平成 24年 4月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00253-011-3814-1 (→Scopusで検索)
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| ○PMID (任意): | □ | 22205442 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1007/s00253-011-3814-1 (日)
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| 2. | (英) Article.Affiliation: Institute of Technology and Science, The University of Tokushima, 2-1 Minamijosanjima-cho, Tokushima 770-8506, Japan. (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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