『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=245292EID:245292, Map:0, LastModified:2015年3月4日(水) 14:23:34, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[木戸 博], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.疾患酵素学研究センター (2007年4月1日〜2016年3月31日/->組織[徳島大学.先端酵素学研究所.次世代酵素学研究領域]) [継承]
著者 (必須): 1.鈴木 宏一 ([大和総研])
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学籍番号 (推奨):
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2.日吉 峰麗
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[継承]
3. (英) Tada Hitomi (日) (読)
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[継承]
4.坂東 美和
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[継承]
5. (英) Ichioka Takao (日) (読)
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学籍番号 (推奨):
[継承]
6.亀村 典生 ([徳島大学.先端酵素学研究所])
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学籍番号 (推奨):
[継承]
7.木戸 博 ([徳島大学.先端酵素学研究所.重点研究部門])
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Allergen diagnosis microarray with high-density immobilization capacity using diamond-like carbon-coated chips for profiling allergen-specific IgE and other immunoglobulins  (日)    [継承]
副題 (任意):
要約 (任意): (英) The diagnosis of antibody-mediated allergic disorders is based on clinical findings, skin prick tests and detection of allergen-specific IgE in serum. Here, we present a new microarray technique of high-density antigen immobilization using carboxylated arms on the surface of a diamond-like carbon (DLC)-coated chip. High immobilization capacity of antigen on DLC chip at (0.94-7.82)×10(9) molecules mm(-2) allowed the analysis of allergen-specific immunoglobulins against not only purified proteins but also natural allergen extracts with wide assay dynamic range. The higher sensitivity of the allergen-specific IgE detection on DLC chip was observed for comparison with the UniCAP system: the DLC chip allowed lowering the limit of dilution rate in UniCAP system to further dilution at 4-8-fold. High correlations (ϱ>0.9-0.85) of allergen-specific IgE values determined by the DLC chip and UniCAP were found in most of 20 different allergens tested. The DLC chip was useful to determine allergen-induced antibodies of IgA, IgG, IgG1, and IgG4 in sera, apart from IgE, as well as secretory IgA in saliva against the same series of allergens on the chip in a minimal amount (1-2 μL) of sample.  (日)    [継承]
キーワード (推奨): 1. (英) Adolescent (日) (読) [継承]
2. (英) Adult (日) (読) [継承]
3. (英) Allergens (日) (読) [継承]
4. (英) Antibody Specificity (日) (読) [継承]
5.子ども (children) [継承]
6. (英) Child, Preschool (日) (読) [継承]
7.ダイヤモンド (diamond) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Immunoassay (日) (読) [継承]
10. (英) Immunoglobulin E (日) (読) [継承]
11.小児 (infant) [継承]
12. (英) Infant, Newborn (日) (読) [継承]
13. (英) Microarray Analysis (日) (読) [継承]
14. (英) Reproducibility of Results (日) (読) [継承]
15. (英) Saliva (日) (読) [継承]
16. (英) Young Adult (日) (読) [継承]
発行所 (推奨): Elsevier (->組織[Elsevier Science]) [継承]
誌名 (必須): Analytica Chimica Acta ([Elsevier Science])
(pISSN: 0003-2670, eISSN: 1873-4324)

ISSN (任意): 1873-4324
ISSN: 0003-2670 (pISSN: 0003-2670, eISSN: 1873-4324)
Title: Analytica chimica acta
Title(ISO): Anal Chim Acta
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 706 [継承]
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(必須): 321 327 [継承]
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年月日 (必須): 西暦 2011年 11月 14日 (平成 23年 11月 14日) [継承]
URL (任意):
DOI (任意): 10.1016/j.aca.2011.08.034    (→Scopusで検索) [継承]
PMID (任意): 22023868    (→Scopusで検索) [継承]
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WOS (任意): 000297184600016 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Koichi Suzuki, Mineyoshi Hiyoshi, Hitomi Tada, Miwa Bando, Takao Ichioka, Norio Kamemura and Hiroshi Kido : Allergen diagnosis microarray with high-density immobilization capacity using diamond-like carbon-coated chips for profiling allergen-specific IgE and other immunoglobulins, Analytica Chimica Acta, Vol.706, No.2, 321-327, 2011.
欧文冊子 ● Koichi Suzuki, Mineyoshi Hiyoshi, Hitomi Tada, Miwa Bando, Takao Ichioka, Norio Kamemura and Hiroshi Kido : Allergen diagnosis microarray with high-density immobilization capacity using diamond-like carbon-coated chips for profiling allergen-specific IgE and other immunoglobulins, Analytica Chimica Acta, Vol.706, No.2, 321-327, 2011.

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