『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Nagae H. (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.中西 秀樹
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Urano Y. (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.荒瀬 誠治
役割 (任意):
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Serial Cultivation of Human Nail Matrix Cells under Serum-free Conditions  (日)    [継承]
副題 (任意):
要約 (任意): (英) We have established serial cultures of human nail matrix cells (NMCs) under serum-free conditions. We cultured NMCs using two different methods depending upon the volume of nail matrix obtained. When a sufficient amount of nail matrix was obtained, they were minced and treated with 0.25% trypsin and 0.03% EDTA. The NMCs were transferred directly as a dispersed cell culture into KGM medium. Because a sufficient amount of matrix was rarely obtained, we developed a method by which NMCs were cultured primarily as implanted small matrices in Eagle's MEM (high Ca+ medium) supplemented with 15% fetal bovine serum for the first 4 to 5 days; during this time, the NMCs expanded from the matrices and formed colonies around them. NMCs then were cultured with KGM. In both methods, KGM medium supported the growth of NMCs without a biological feeder layer. These cells could be cultivated serially for at least seven passages. Half of the cells were positively stained with a monoclonal antibody against hair (hard) keratin which is expressed in nail matrix in vivo, indicating that the cells originated from the nail matrix. These methods will now permit investigations of nail matrix cells that previously were unfeasible because of the relative lack of cells and difficulties with propagation.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2.カルシウム (calcium) [継承]
3. (英) Cattle (日) (読) [継承]
4. (英) Cell Adhesion (日) (読) [継承]
5. (英) Cell Count (日) (読) [継承]
6. (英) Cell Culture Techniques (日) (読) [継承]
7.細胞分裂 (cell division) [継承]
8. (英) Cells, Cultured (日) (読) [継承]
9. (英) Culture Media (日) (読) [継承]
10. (英) Culture Media, Serum-Free (日) (読) [継承]
11. (英) Edetic Acid (日) (読) [継承]
12. (英) Fluorescent Antibody Technique, Indirect (日) (読) [継承]
13. (英) Humans (日) (読) [継承]
14.免疫組織化学 (immunohistochemistry) [継承]
15. (英) Keratins (日) (読) [継承]
16. (英) Nails (日) (読) [継承]
17.トリプシン (trypsin) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Dermatology (日本皮膚科学会)
(pISSN: 0385-2407, eISSN: 1346-8138)

ISSN (任意): 0385-2407
ISSN: 0385-2407 (pISSN: 0385-2407, eISSN: 1346-8138)
Title: The Journal of dermatology
Title(ISO): J Dermatol
Supplier: Japanese Dermatological Association
Publisher: Wiley Publishing
 (NLM Catalog  (医中誌Web  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 22 [継承]
(必須): 8 [継承]
(必須): 560 566 [継承]
都市 (任意):
年月日 (必須): 西暦 1995年 8月 初日 (平成 7年 8月 初日) [継承]
URL (任意):
DOI (任意):
PMID (任意): 7560452    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意): 2-s2.0-0029095932 [継承]
評価値 (任意):
被引用数 (任意):
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● H. Nagae, Hideki Nakanishi, Y. Urano and Seiji Arase : Serial Cultivation of Human Nail Matrix Cells under Serum-free Conditions, The Journal of Dermatology, Vol.22, No.8, 560-566, 1995.
欧文冊子 ● H. Nagae, Hideki Nakanishi, Y. Urano and Seiji Arase : Serial Cultivation of Human Nail Matrix Cells under Serum-free Conditions, The Journal of Dermatology, Vol.22, No.8, 560-566, 1995.

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