『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=237306)

EID=237306EID:237306, Map:0, LastModified:2012年9月24日(月) 11:45:02, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[堤 理恵], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.堤 理恵 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.代謝栄養学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Mistry Devendra (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Webster J. G. Nicholas (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Signaling responses to pulsatile gonadotropin-releasing hormone in LbetaT2 gonadotrope cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The hypothalamic neuropeptide gonadotropin-releasing hormone (GnRH) is secreted in a pulsatile fashion by hypothalamic neurons, and alterations in pulse frequency and amplitude differentially regulate gonadotropin synthesis and release. In this study, we investigated the kinetics of G(s) and G(q) signaling in response to continuous or pulsatile GnRH using fluorescence resonance energy transfer reporters in live mouse LbetaT2 gonadotrope cells. cAMP and protein kinase A-dependent reporters showed a rapid but transient increase in fluorescence resonance energy transfer signal with increasing doses of constant GnRH, and in contrast diacylglycerol (DAG) and calcium reporters showed a rapid and sustained signal. Multiple pulses of GnRH caused multiple pulses of cAMP and protein kinase A activation without desensitization, but the DAG and calcium reporters were rapidly desensitized resulting in inhibition of calcium and DAG responses. At the transcriptional level, both a cAMP-dependent cAMP-response element reporter and a DAG/calcium-dependent AP-1 reporter showed a pulse frequency-dependent increase in luciferase activity. However, constant GnRH stimulation gave very little cAMP-response element activation but very strong AP-1 activation. Based on these data, we propose that both the GnRH-R-G(s) and G(q) pathways are responsive to pulses of GnRH, but only the G(q) pathway is responsive to constant GnRH. Furthermore, the G(q) pathway is subject to desensitization with multiple GnRH pulses, but the G(s) pathway is not.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Calcium (日) (読) [継承]
3. (英) Cell Line (日) (読) [継承]
4. (英) Cyclic AMP (日) (読) [継承]
5. (英) Cyclic AMP Response Element-Binding Protein (日) (読) [継承]
6. (英) Cyclic AMP-Dependent Protein Kinases (日) (読) [継承]
7. (英) Diglycerides (日) (読) [継承]
8. (英) Fluorescence Resonance Energy Transfer (日) (読) [継承]
9. (英) Gonadotrophs (日) (読) [継承]
10. (英) Gonadotropin-Releasing Hormone (日) (読) [継承]
11. (英) Immunoblotting (日) (読) [継承]
12. (英) Intracellular Space (日) (読) [継承]
13. (英) Kinetics (日) (読) [継承]
14. (英) Luciferases (日) (読) [継承]
15. (英) Mice (日) (読) [継承]
16. (英) Models, Biological (日) (読) [継承]
17.リン酸化 (phosphorylation) [継承]
18.情報伝達 (signal transduction/->キーワード[シグナル伝達]) [継承]
19. (英) Transcription Factor AP-1 (日) (読) [継承]
20. (英) Transcriptional Activation (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

ISSN (任意): 1083-351X
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 285 [継承]
(必須): 26 [継承]
(必須): 20262 20272 [継承]
都市 (任意):
年月日 (必須): 西暦 2010年 4月 20日 (平成 22年 4月 20日) [継承]
URL (任意):
DOI (任意): 10.1074/jbc.M110.132662    (→Scopusで検索) [継承]
PMID (任意): 20406815    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Medical Research Service, Veterans Affairs San Diego Healthcare System, San Diego, California 92161, USA.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural  (日)    [継承]
4.(英) OtherID: PMC2888439  (日)    [継承]

標準的な表示

和文冊子 ● Rie Tsutsumi, Devendra Mistry and Nicholas J. G. Webster : Signaling responses to pulsatile gonadotropin-releasing hormone in LbetaT2 gonadotrope cells., The Journal of Biological Chemistry, Vol.285, No.26, 20262-20272, 2010.
欧文冊子 ● Rie Tsutsumi, Devendra Mistry and Nicholas J. G. Webster : Signaling responses to pulsatile gonadotropin-releasing hormone in LbetaT2 gonadotrope cells., The Journal of Biological Chemistry, Vol.285, No.26, 20262-20272, 2010.

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