『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
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組織 (推奨):
著者 (必須): 1.美馬 晶
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2.安部 秀斉
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3.長井 幸二郎
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4. (英) Arai Hidenori (日) (読)
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5. (英) Matsubara Takeshi (日) (読)
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6. (英) Araki Makoto (日) (読)
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7. (英) Torikoshi Kazuo (日) (読)
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8.冨永 辰也 ([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.医用検査学系.生体機能解析学])
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9. (英) Iehara Noriyuki (日) (読)
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10. (英) Fukatsu Atsushi (日) (読)
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11. (英) Kita Toru (日) (読)
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12.土井 俊夫
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題名 (必須): (英) Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Platelet-derived growth factor (PDGF) plays critical roles in mesangial cell (MC) proliferation in mesangial proliferative glomerulonephritis. We showed previously that Smad1 contributes to PDGF-dependent proliferation of MCs, but the mechanism by which Smad1 is activated by PDGF is not precisely known. Here we examined the role of c-Src tyrosine kinase in the proliferative change of MCs. Experimental mesangial proliferative glomerulonephritis (Thy1 GN) was induced by a single intravenous injection of anti-rat Thy-1.1 monoclonal antibody. In Thy1 GN, MC proliferation and type IV collagen (Col4) expression peaked on day 6. Immunohistochemical staining for the expression of phospho-Src (pSrc), phospho-Smad1 (pSmad1), Col4, and smooth muscle -actin (SMA) revealed that the activation of c-Src and Smad1 signals in glomeruli peaked on day 6, consistent with the peak of mesangial proliferation. When treated with PP2, a Src inhibitor, both mesangial proliferation and sclerosis were significantly reduced. PP2 administration also significantly reduced pSmad1, Col4, and SMA expression. PDGF induced Col4 synthesis in association with increased expression of pSrc and pSmad1 in cultured MCs. In addition, PP2 reduced Col4 synthesis along with decreased pSrc and pSmad1 protein expression in vitro. Moreover, the addition of siRNA against c-Src significantly reduced the phosphorylation of Smad1 and the overproduction of Col4. These results provide new evidence that the activation of Src/Smad1 signaling pathway plays a key role in the development of glomerulosclerosis in experimental glomerulonephritis.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Blotting, Western (日) (読) [継承]
3. (英) Disease Progression (日) (読) [継承]
4. (英) Glomerulonephritis (日) (読) [継承]
5.免疫組織化学 (immunohistochemistry) [継承]
6. (英) Mice (日) (読) [継承]
7. (英) Mice, Inbred C57BL (日) (読) [継承]
8.リン酸化 (phosphorylation) [継承]
9. (英) Platelet-Derived Growth Factor (日) (読) [継承]
10. (英) Proto-Oncogene Proteins pp60(c-src) (日) (読) [継承]
11. (英) RNA, Small Interfering (日) (読) [継承]
12.シグナル伝達 (signal transduction) [継承]
13. (英) Smad1 Protein (日) (読) [継承]
発行所 (推奨):
誌名 (必須): PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)

ISSN (任意): 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: PLOS
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 6 [継承]
(必須): 3 [継承]
(必須): e17929 e17929 [継承]
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年月日 (必須): 西暦 2011年 3月 22日 (平成 23年 3月 22日) [継承]
URL (任意):
DOI (任意): 10.1371/journal.pone.0017929    (→Scopusで検索) [継承]
PMID (任意): 21445358    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Nephrology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC3062564  (日)    [継承]

標準的な表示

和文冊子 ● Akira Mima, Hideharu Abe, Kojiro Nagai, Hidenori Arai, Takeshi Matsubara, Makoto Araki, Kazuo Torikoshi, Tatsuya Tominaga, Noriyuki Iehara, Atsushi Fukatsu, Toru Kita and Toshio Doi : Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis., PLoS ONE, Vol.6, No.3, e17929, 2011.
欧文冊子 ● Akira Mima, Hideharu Abe, Kojiro Nagai, Hidenori Arai, Takeshi Matsubara, Makoto Araki, Kazuo Torikoshi, Tatsuya Tominaga, Noriyuki Iehara, Atsushi Fukatsu, Toru Kita and Toshio Doi : Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis., PLoS ONE, Vol.6, No.3, e17929, 2011.

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