『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Wójcik Cezary (日) (読)
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2.矢野 仁康
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3. (英) DeMartino N. George (日) (読)
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題名 (必須): (英) RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis  (日)    [継承]
副題 (任意):
要約 (任意): (英) We have used RNA interference (RNAi) to examine the functional relationship between valosin-containing protein (VCP/p97/Cdc48p/TER94) ATPase and the ubiquitin-proteasome system (UPS) in Drosophila S2 and human HeLa cells. In both cell types, RNAi of VCP (and, to a lesser extent, of certain VCP-interacting proteins) caused significant accumulation of high-molecular-weight conjugates of ubiquitin, an indication of inhibited UPS function. However, decreased VCP levels did not directly inhibit proteasome activity. In HeLa cells, polyubiquitinated proteins accumulated as dispersed aggregates rather than as single aggresomes, even in the presence of proteasome inhibitors, which normally promote aggresome formation. RNAi of VCP caused extensive vacuolization of the cytoplasm, and proteasome inhibitors exaggerated this feature. RNAi of VCP had little effect on S2 cell proliferation but blocked cell-cycle progression and induced mitotic abnormalities and apoptosis in HeLa cells. These results indicate that VCP plays an important general role in mediating the function of the UPS, probably by interacting with potential proteasome substrates before they are degraded by the proteasome.  (日)    [継承]
キーワード (推奨): 1. (英) Adenosine Triphosphatases (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3.アポトーシス (apoptosis) [継承]
4. (英) Caspases (日) (読) [継承]
5.細胞周期 (cell cycle) [継承]
6. (英) Cell Cycle Proteins (日) (読) [継承]
7.細胞分裂 (cell division) [継承]
8. (英) Cells, Cultured (日) (読) [継承]
9. (英) Cysteine Endopeptidases (日) (読) [継承]
10. (英) Drosophila (日) (読) [継承]
11. (英) Flow Cytometry (日) (読) [継承]
12. (英) HeLa Cells (日) (読) [継承]
13. (英) Humans (日) (読) [継承]
14. (英) Microscopy, Fluorescence (日) (読) [継承]
15. (英) Multienzyme Complexes (日) (読) [継承]
16. (英) Proteasome Endopeptidase Complex (日) (読) [継承]
17. (英) RNA, Small Interfering (日) (読) [継承]
18. (英) Ubiquitin (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Cell Science ([The Company of Biologists Limited])
(pISSN: 0021-9533, eISSN: 1477-9137)

ISSN (任意): 0021-9533
ISSN: 0021-9533 (pISSN: 0021-9533, eISSN: 1477-9137)
Title: Journal of cell science
Title(ISO): J Cell Sci
Publisher: Company of Biologists
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年月日 (必須): 西暦 2003年 12月 2日 (平成 15年 12月 2日) [継承]
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DOI (任意): 10.1242/jcs.00841    (→Scopusで検索) [継承]
PMID (任意): 14657277    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9040, USA.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.  (日)    [継承]

標準的な表示

和文冊子 ● Cezary Wójcik, Mihiro Yano and George N. DeMartino : RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis, Journal of Cell Science, Vol.117, No.2, 281-292, 2003.
欧文冊子 ● Cezary Wójcik, Mihiro Yano and George N. DeMartino : RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis, Journal of Cell Science, Vol.117, No.2, 281-292, 2003.

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