『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須):
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Matsumoto Natsuki (日) (読)
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[継承]
2. (英) Hemmi Akihiro (日) (読)
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[継承]
3. (英) Yamato Hideyuki (日) (読)
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[継承]
4. (英) Ohnishi Ritsuko (日) (読)
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[継承]
5. (英) Segawa Hiroko (日) (読)
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[継承]
6. (英) Ohno Shinichi (日) (読)
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[継承]
7.宮本 賢一
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Immunohistochemical analyses of parathyroid hormone-dependent downregulation of renal type II Na-Pi cotransporters by cryobiopsy.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The "in vivo cryotechnique" (IVCT) is a new method of morphological analysis which has the advantage of freezing tissues in living animals without stopping their blood circulation. The purpose of this study was to investigate the effect of parathyroid hormone (PTH) on renal type II Na-Pi transporters (NaPi-IIa and NaPi-IIc) and "cryobiopsy" (CB) using special cryoforceps as a simple method of the IVCT. The kidney tissues were biopsied at various time points after PTH administration by CB using liquid nitrogen as the cryogen. By hematoxylin-eosin (HE) staining the kidney tissues, well-frozen areas without visible ice crystals were obtained in the tissue surface areas, and the brush border membrane (BBM) of proximal tubules was well preserved at a light microscopic level. Immunohistochemical evaluation showed that PTH downregulated NaPi-IIa and NaPi-IIc at the BBM, being controlled by a different mechanism. In this method, the PTH-induced internalization of NaPi-IIc from microvilli to subapical compartments was not observed in the tissue preparations. NaPi-IIc protein appears to be degraded in microvilli of the proximal tubular cells after the injection of PTH. We suggest that CB using liquid nitrogen is useful to investigate renal type II Na-Pi transporters at the light microscopic level.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Biopsy (日) (読) [継承]
3. (英) Down-Regulation (日) (読) [継承]
4.免疫組織化学 (immunohistochemistry) [継承]
5. (英) Kidney (日) (読) [継承]
6. (英) Male (日) (読) [継承]
7. (英) Parathyroid Hormone (日) (読) [継承]
8. (英) Rats (日) (読) [継承]
9. (英) Rats, Wistar (日) (読) [継承]
10. (英) Sodium-Phosphate Cotransporter Proteins, Type II (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1349-6867
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J Med Invest
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 57 [継承]
(必須): 1-2 [継承]
(必須): 138 145 [継承]
都市 (任意):
年月日 (必須): 西暦 2010年 2月 初日 (平成 22年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.2152/jmi.57.138    (→Scopusで検索) [継承]
PMID (任意): 20299753    (→Scopusで検索) [継承]
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機関リポジトリ : 77536 [継承]
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備考 (任意): 1.(英) Affiliation: Kureha Special Laboratory Co., Ltd., Shinjyuku, Tokyo, Japan.  (日)    [継承]
2.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● (種別) : Natsuki Matsumoto, Akihiro Hemmi, Hideyuki Yamato, Ritsuko Ohnishi, Hiroko Segawa, Shinichi Ohno and Ken-ichi Miyamoto : Immunohistochemical analyses of parathyroid hormone-dependent downregulation of renal type II Na-Pi cotransporters by cryobiopsy., The Journal of Medical Investigation : JMI, Vol.57, No.1-2, 138-145, (発行所), (都市), Feb. 2010.
欧文冊子 ● (種別) : Natsuki Matsumoto, Akihiro Hemmi, Hideyuki Yamato, Ritsuko Ohnishi, Hiroko Segawa, Shinichi Ohno and Ken-ichi Miyamoto : Immunohistochemical analyses of parathyroid hormone-dependent downregulation of renal type II Na-Pi cotransporters by cryobiopsy., The Journal of Medical Investigation : JMI, Vol.57, No.1-2, 138-145, (発行所), (都市), Feb. 2010.

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