| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Haraguchi Kyoko (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 2. | (英) Suzuki Takahiro (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | (英) Koyama Noriko (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | (英) Kumano Keiki (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | (英) Nakahara Fumio (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | (英) Matsumoto Akihiko (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 7. | (英) Yokoyama Yasuhisa (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 8. | (英) Sakata-Yanagimoto Mamiko (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 9. | (英) Masuda Shigeo (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 10. | (英) Takahashi Tsuyoshi (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 11. | (英) Kamijo Aki (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 12. | (英) Takahashi Koki (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 13. | (英) Takanashi Minoko (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 14. | (英) Okuyama Yoshiki (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 15. | 安友 康二 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 16. | (英) Sakano Seiji (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 17. | (英) Yagita Hideo (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 18. | (英) Kurokawa Mineo (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 19. | (英) Ogawa Seishi (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 20. | (英) Chiba Shigeru (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15. (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) The development of NK cells from hematopoietic stem cells is thought to be dependent on IL-15. In this study, we demonstrate that stimulation of human cord blood CD34(+) cells by a Notch ligand, Delta4, along with IL-7, stem cell factor, and Fms-like tyrosine kinase 3 ligand, but no IL-15, in a stroma-free culture induced the generation of cells with characteristics of functional NK cells, including CD56 and CD161 Ag expression, IFN-gamma secretion, and cytotoxic activity against K562 and Jurkat cells. Addition of gamma-secretase inhibitor and anti-human Notch1 Ab to the culture medium almost completely blocked NK cell emergence. Addition of anti-human IL-15-neutralizing Ab did not affect NK cell development in these culture conditions. The presence of IL-15, however, augmented cytotoxicity and was required for a more mature NK cell phenotype. CD56(+) cells generated by culture with IL-15, but without Notch stimulation, were negative for CD7 and cytoplasmic CD3, whereas CD56(+) cells generated by culture with both Delta4 and IL-15 were CD7(+) and cytoplasmic CD3(+) from the beginning and therefore more similar to in vivo human NK cell progenitors. Together, these results suggest that Notch signaling is important for the physiologic development of NK cells at differentiation stages beyond those previously postulated. (日)
| [継承] |
| ○キーワード (推奨): | 1. | (英) Antigens, CD34 (日) (読)
| [継承] |
| 2. | (英) Cell Differentiation (日) (読)
| [継承] |
| 3. | (英) Fetal Blood (日) (読)
| [継承] |
| 4. | (英) Flow Cytometry (日) (読)
| [継承] |
| 5. | (英) Fluorescent Antibody Technique (日) (読)
| [継承] |
| 6. | (英) Hematopoietic Stem Cells (日) (読)
| [継承] |
| 7. | (英) Humans (日) (読)
| [継承] |
| 8. | (英) Interleukin-15 (日) (読)
| [継承] |
| 9. | (英) Killer Cells, Natural (日) (読)
| [継承] |
| 10. | (英) Lymphocyte Subsets (日) (読)
| [継承] |
| 11. | (英) Receptors, Notch (日) (読)
| [継承] |
| 12. | (英) Signal Transduction (日) (読)
| [継承] |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)
| ○ISSN (任意): | □ | 1550-6606
ISSN: 0022-1767
(pISSN: 0022-1767, eISSN: 1550-6606) Title: Journal of immunology (Baltimore, Md. : 1950)Title(ISO): J ImmunolPublisher: American Association of Immunologists (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 182
| [継承] |
| ○号 (必須): | □ | 10
| [継承] |
| ○頁 (必須): | □ | 6168 6178
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2009年 5月 15日 (平成 21年 5月 15日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.4049/jimmunol.0803036 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 19414770 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Cell Therapy and Transplantation Medicine, University of Tokyo Hospital, Tokyo, Japan. (日)
| [継承] |
| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
| [継承] |
| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
| [継承] |