『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.疾患酵素学研究センター (2007年4月1日〜2016年3月31日/->組織[徳島大学.先端酵素学研究所.次世代酵素学研究領域]) [継承]
著者 (必須): 1. (英) Mine Akira (日) (読)
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2. (英) Takeda Atsushi (日) (読)
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3.谷口 貴子
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4.谷口 寿章
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5. (英) Kaido Masanori (日) (読)
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6. (英) Mise Kazuyuki (日) (読)
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7. (英) Okuno Tetsuro (日) (読)
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題名 (必須): (英) Identification and characterization of the 480-kilodalton template-specific RNA-dependent RNA polymerase complex of red clover necrotic mosaic virus.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Replication of positive-strand RNA viruses occurs through the assembly of membrane-associated viral RNA replication complexes that include viral replicase proteins, viral RNA templates, and host proteins. Red clover necrotic mosaic virus (RCNMV) is a positive-strand RNA plant virus with a genome consisting of RNA1 and RNA2. The two proteins encoded by RNA1, a 27-kDa protein (p27) and an 88-kDa protein containing an RNA-dependent RNA polymerase (RdRP) motif (p88), are essential for RCNMV RNA replication. To analyze RCNMV RNA replication complexes, we used blue-native polyacrylamide gel electrophoresis (BN/PAGE), which enabled us to analyze detergent-solubilized large membrane protein complexes. p27 and p88 formed a complex of 480 kDa in RCNMV-infected plants. As a result of sucrose gradient sedimentation, the 480-kDa complex cofractionated with both endogenous template-bound and exogenous template-dependent RdRP activities. The amount of the 480-kDa complex corresponded to the activity of exogenous template-dependent RdRP, which produced RNA fragments by specifically recognizing the 3'-terminal core promoter sequences of RCNMV RNAs, but did not correspond to the activity of endogenous template-bound RdRP, which produced genome-sized RNAs without the addition of RNA templates. These results suggest that the 480-kDa complex contributes to template-dependent RdRP activities. We subjected those RdRP complexes to affinity purification and analyzed their components using two-dimensional BN/sodium dodecyl sulfate-PAGE (BN/SDS-PAGE) and mass spectrometry. The 480-kDa complex contained p27, p88, and possible host proteins, and the original affinity-purified RdRP preparation contained HSP70, HSP90, and several ribosomal proteins that were not detected in the 480-kDa complex. A model for the formation of RCNMV RNA replication complexes is proposed.  (日)    [継承]
キーワード (推奨): 1. (英) Host-Parasite Interactions (日) (読) [継承]
2. (英) Macromolecular Substances (日) (読) [継承]
3. (英) Molecular Weight (日) (読) [継承]
4. (英) Plant Diseases (日) (読) [継承]
5. (英) RNA Replicase (日) (読) [継承]
6. (英) RNA, Viral (日) (読) [継承]
7. (英) Templates, Genetic (日) (読) [継承]
8. (英) Tombusviridae (日) (読) [継承]
9. (英) Viral Proteins (日) (読) [継承]
10. (英) Virus Replication (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Virology ([アメリカ微生物学会])
(pISSN: 0022-538X, eISSN: 1098-5514)

ISSN (任意): 1098-5514
ISSN: 0022-538X (pISSN: 0022-538X, eISSN: 1098-5514)
Title: Journal of virology
Title(ISO): J Virol
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 84 [継承]
(必須): 12 [継承]
(必須): 6070 6081 [継承]
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年月日 (必須): 西暦 2010年 4月 7日 (平成 22年 4月 7日) [継承]
URL (任意):
DOI (任意): 10.1128/JVI.00054-10    (→Scopusで検索) [継承]
PMID (任意): 20375154    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kitashirakawa, Kyoto 606-8502, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC2876630  (日)    [継承]

標準的な表示

和文冊子 ● Akira Mine, Atsushi Takeda, Takako Taniguchi, Hisaaki Taniguchi, Masanori Kaido, Kazuyuki Mise and Tetsuro Okuno : Identification and characterization of the 480-kilodalton template-specific RNA-dependent RNA polymerase complex of red clover necrotic mosaic virus., Journal of Virology, Vol.84, No.12, 6070-6081, 2010.
欧文冊子 ● Akira Mine, Atsushi Takeda, Takako Taniguchi, Hisaaki Taniguchi, Masanori Kaido, Kazuyuki Mise and Tetsuro Okuno : Identification and characterization of the 480-kilodalton template-specific RNA-dependent RNA polymerase complex of red clover necrotic mosaic virus., Journal of Virology, Vol.84, No.12, 6070-6081, 2010.

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