『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=230036EID:230036, Map:0, LastModified:2013年10月15日(火) 15:07:29, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[細井 和雄], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座.口腔分子生理学 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1.長谷川 敬展 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
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[継承]
2. (英) Azlina, Ahmad (日) Azlina, Ahmad (読)
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[継承]
3.フルジャフ ジャフラン
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[継承]
4.姚 陳娟 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
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学籍番号 (推奨):
[継承]
5.赤松 徹也 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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[継承]
6.細井 和雄
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Novel phosphorylation of aquaporin-5 at its threonine 259 through cAMP signaling  (日)    [継承]
副題 (任意):
要約 (任意): (英) Aquaporin-5 (AQP5), a water channel, plays key roles in salivary secretion. The novel phosphorylation of AQP5 was investigated by using human salivary gland (HSG) cells and mouse salivary glands. In the HSG cells stably transfected with a wild-type mouse AQP5 construct, a protein band immunoreactive with antibody against phosphorylated PKA substrate was detected in the AQP5 immunoprecipitated sample, and its intensity was enhanced by short-term treatment of the cells with 8-bromo-cAMP, forskolin, or phorbol 12-myristate 13-acetate, but not by that with A23187 calcium ionophore. Such enhancement was inhibited in the presence of H-89, a PKA inhibitor. An AQP5 mutant (AQP5-T259A) expressed by transfection of HSG cells was not recognized by anti-phosphorylated PKA substrate antibody, even when the cells were stimulated with the protein kinase activators. Immunoblotting and immunofluorescence studies using a specific antibody detecting AQP5 phosphorylated at its Thr259 demonstrated that AQP5 was rapidly and transiently phosphorylated at the apical membrane of acinar cells in the submandibular and parotid glands after administration of isoproterenol, but not pilocarpine. Furthermore, both AQP5 and AQP5-T259A were constitutively localized at the plasma membrane in HSG cells under the resting and forskolin-stimulated conditions. These results suggest that AQP5 is phosphorylated at its Thr259 by PKA through cAMP, but not Ca(2+), signaling pathways, and that this phosphorylation does not contribute to AQP5 trafficking in the salivary gland cells.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Substitution (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antibodies (日) (読) [継承]
4. (英) Antibody Specificity (日) (読) [継承]
5. (英) Aquaporin 5 (日) (読) [継承]
6. (英) Calcimycin (日) (読) [継承]
7. (英) Cell Line, Tumor (日) (読) [継承]
8. (英) Cell Membrane (日) (読) [継承]
9. (英) Cyclic AMP (日) (読) [継承]
10. (英) Cyclic AMP-Dependent Protein Kinases (日) (読) [継承]
11. (英) Detergents (日) (読) [継承]
12. (英) Forskolin (日) (読) [継承]
13. (英) Humans (日) (読) [継承]
14. (英) Isoproterenol (日) (読) [継承]
15. (英) Isoquinolines (日) (読) [継承]
16. (英) Male (日) (読) [継承]
17. (英) Mice (日) (読) [継承]
18. (英) Mice, Inbred ICR (日) (読) [継承]
19. (英) Parotid Gland (日) (読) [継承]
20. (英) Phosphorylation (日) (読) [継承]
21. (英) Pilocarpine (日) (読) [継承]
22. (英) Protein Kinase Inhibitors (日) (読) [継承]
23. (英) Protein Transport (日) (読) [継承]
24. (英) Salivary Glands (日) (読) [継承]
25. (英) Signal Transduction (日) (読) [継承]
26. (英) Submandibular Gland (日) (読) [継承]
27. (英) Sulfonamides (日) (読) [継承]
28. (英) Tetradecanoylphorbol Acetate (日) (読) [継承]
29. (英) Threonine (日) (読) [継承]
30. (英) Transfection (日) (読) [継承]
発行所 (推奨): アメリカ生理学会 [継承]
誌名 (必須): American Journal of Physiology, Cell Physiology ([アメリカ生理学会])
(pISSN: 0363-6143, eISSN: 1522-1563)

ISSN (任意): 1522-1563
ISSN: 0363-6143 (pISSN: 0363-6143, eISSN: 1522-1563)
Title: American journal of physiology. Cell physiology
Title(ISO): Am J Physiol Cell Physiol
Publisher: American Physiological Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 301 [継承]
(必須): 3 [継承]
(必須): C667 C678 [継承]
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年月日 (必須): 西暦 2011年 9月 初日 (平成 23年 9月 初日) [継承]
URL (任意):
DOI (任意): 10.1152/ajpcell.00058.2011    (→Scopusで検索) [継承]
PMID (任意): 21633078    (→Scopusで検索) [継承]
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WOS (任意): 000294339400016 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Dept. of Molecular Oral Physiology, Institute of Health Biosciences, The Univ. of Tokushima Graduate School, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Takahiro Hasegawa, Ahmad Azlina, Javkhlan Purevjav, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi : Novel phosphorylation of aquaporin-5 at its threonine 259 through cAMP signaling, American Journal of Physiology, Cell Physiology, Vol.301, No.3, C667-C678, 2011.
欧文冊子 ● Takahiro Hasegawa, Ahmad Azlina, Javkhlan Purevjav, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi : Novel phosphorylation of aquaporin-5 at its threonine 259 through cAMP signaling, American Journal of Physiology, Cell Physiology, Vol.301, No.3, C667-C678, 2011.

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