『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=229945)

EID=229945EID:229945, Map:0, LastModified:2012年10月17日(水) 15:48:28, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[玉谷 哲也], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kawamata Hitoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.表原 文江
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Nakashiro Koh-Ichi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.内田 大亮
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Shinagawa Yasuhiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Tachibana Masatsugu (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Imai Yutaka (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Fujimori Takahiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Oncogenic mutation of the p53 gene derived from head and neck cancer prevents cells from undergoing apoptosis after DNA damage.  (日)    [継承]
副題 (任意):
要約 (任意): (英) A p53 functional analysis system, which can identify the types of abnormality of p53, such as loss of function, dominant negative function, or gain of oncogenic function, is now required. In this study, we examined the functional diversity of several mutations of p53 derived from human head and neck cancer cells. The entire open reading frame of p53 cDNA was subcloned into a mammalian expression vector, pEGFP-C3, and genetic mutations were determined. Then, intracellular localization and transcriptional activity of the tumor-derived p53 proteins were examined in Saos-2 cells. A mutant-p53 (Glu17Lys, His193Leu) or a truncated p53 (Delta121) did not activate the reporters containing p53 responsive elements from p21waf1, BAX, MDM2, p53AIP1, and PUMA genes at all. However, a mutant-p53 (Asn30Ser) showed the transcriptional activity on all of the reporters as wild-type p53 did. On the other hand, a mutant-p53 (Asp281His) activated the p21waf1 promoter strongly and the MDM2 promoter faintly, but did not activate the BAX promoter. Interestingly, this mutant-p53 prevented Saos-2 cells from undergoing apoptosis after treatment with a DNA damaging agent, adriamycin. This mutant-p53 induced cell cycle arrest but not apoptosis. Furthermore, another mutant-p53 (Glu17Lys, His193Leu) also prevented the cells from undergoing apoptosis after DNA damage probably in a transcription-independent manner. These results suggest that some cancer cells may contain the oncogenic mutation of the p53 gene, and the oncogenic p53 protein prevents cancer cells from undergoing apoptosis after DNA damage. Detailed information for mutated p53 gene in cancer cells might provide useful suggestions for the therapeutic strategy.  (日)    [継承]
キーワード (推奨): 1. (英) Apoptosis (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Cell Line (日) (読) [継承]
5. (英) Cell Line, Tumor (日) (読) [継承]
6. (英) Cell Survival (日) (読) [継承]
7. (英) DNA Damage (日) (読) [継承]
8. (英) DNA, Complementary (日) (読) [継承]
9. (英) Genes, p53 (日) (読) [継承]
10. (英) Head and Neck Neoplasms (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Molecular Sequence Data (日) (読) [継承]
13. (英) Mutation (日) (読) [継承]
14. (英) Open Reading Frames (日) (読) [継承]
15. (英) Tumor Suppressor Protein p53 (日) (読) [継承]
発行所 (推奨):
誌名 (必須): International Journal of Oncology (International Center for Cancer Research)
(pISSN: 1019-6439, eISSN: 1791-2423)

ISSN (任意): 1019-6439
ISSN: 1019-6439 (pISSN: 1019-6439, eISSN: 1791-2423)
Title: International journal of oncology
Title(ISO): Int. J. Oncol.
Publisher: Spandidos Publications
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 30 [継承]
(必須): 5 [継承]
(必須): 1089 1097 [継承]
都市 (任意):
年月日 (必須): 西暦 2007年 5月 初日 (平成 19年 5月 初日) [継承]
URL (任意):
DOI (任意):
PMID (任意): 17390010    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000245903900006 [継承]
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Department of Surgical and Molecular Pathology, Dokkyo University School of Medicine, Mibu, Shimo-Tsuga, Tochigi, Japan. h-kawama@dokkyomed.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hitoshi Kawamata, Fumie Omotehara, Koh-Ichi Nakashiro, Daisuke Uchida, Yasuhiro Shinagawa, Masatsugu Tachibana, Yutaka Imai and Takahiro Fujimori : Oncogenic mutation of the p53 gene derived from head and neck cancer prevents cells from undergoing apoptosis after DNA damage., International Journal of Oncology, Vol.30, No.5, 1089-1097, 2007.
欧文冊子 ● Hitoshi Kawamata, Fumie Omotehara, Koh-Ichi Nakashiro, Daisuke Uchida, Yasuhiro Shinagawa, Masatsugu Tachibana, Yutaka Imai and Takahiro Fujimori : Oncogenic mutation of the p53 gene derived from head and neck cancer prevents cells from undergoing apoptosis after DNA damage., International Journal of Oncology, Vol.30, No.5, 1089-1097, 2007.

関連情報

Number of session users = 0, LA = 0.50, Max(EID) = 360752, Max(EOID) = 966299.