『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
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組織 (推奨):
著者 (必須): 1.玉谷 哲也
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2.東 雅之
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3.茂木 勝美
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4.髙丸 菜都美 ([徳島大学.病院.診療科.歯科口腔外科.口腔外科])
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5. (英) Kawashima Yuichiro (日) (読)
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6.板東 髙志
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題名 (必須): (英) Cepharanthin-enhanced radiosensitivity through the inhibition of radiation-induced nuclear factor-kappaB activity in human oral squamous cell carcinoma cells.  (日)    [継承]
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要約 (任意): (英) We have already demonstrated that human head and neck cancer cells have significantly enhanced levels of transcription factor nuclear factor (NF)-kappaB activity compared to their normal counterparts, suggesting that NF-kappaB plays an important role in the development of head and neck cancer. However, it has been reported that chemotherapeutic agents and radiation activate NF-kappaB activity in cancer cells, thus making the cells radioresistant and chemoresistant. In addition, we have shown that the suppression of NF-kappaB activity enhanced apoptosis in oral squamous cell carcinoma cells. In this study, we examined whether cepharanthin-induced inhibition of NF-kappaB activity enhances radiosensitivity in human oral carcinoma cells. Cepharanthin is a biscoclaurine alkaloid extracted from the roots of Stephania cepharantha hayata, and is widely used in Japan for the treatment of patients with leucopenia, nasal allergy, and venomous snakebites. gamma-irradiation (IR) induces NF-kappaB activity in oral carcinoma cells through the activation of upstream molecules, including Akt and IkappaB kinase. However, a luciferase assay revealed that cepharanthin suppresses IR-induced NF-kappaB activity in oral squamous cell carcinoma cells, thereby enhancing the radio-sensitivity. Western blot analysis showed an enhanced cleavage of poly-(ADP-ribose) polymerase protein in carcinoma cells by both cepharanthin treatment and IR exposure compared to IR or cepharanthin alone. In an in vivo study, B88 cells were s.c. inoculated into the backs of nude mice. Tumor-bearing nude mice received either cepharanthin, IR alone, or a combination of cepharanthin and IR. The combined treatment suppressed tumor growth significantly more than either cepharanthin or IR alone. Cepharanthin inhibited the production of IR-induced IL-6 and IL-8, which are downstream targets of NF-kappaB. In quantitative real-time RT-PCR, IR also induced the expression of anti-apoptotic proteins [cellular inhibitor of apoptosis protein (cIAP)-1 and -2] in carcinoma cells. Treatment of cancer cells with cepharanthin combined with exposure to IR decreased cIAP-1 and -2 mRNA expression. These findings suggested that the combination of radiotherapy and cepharanthin could enhance radiosensitivity in the treatment of human oral cancer.  (日)    [継承]
キーワード (推奨): 1. (英) Alkaloids (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Apoptosis (日) (読) [継承]
4. (英) Benzylisoquinolines (日) (読) [継承]
5. (英) Blotting, Western (日) (読) [継承]
6. (英) Carcinoma, Squamous Cell (日) (読) [継承]
7. (英) Cell Line, Tumor (日) (読) [継承]
8. (英) Fluorescent Antibody Technique (日) (読) [継承]
9. (英) Gamma Rays (日) (読) [継承]
10. (英) Humans (日) (読) [継承]
11. (英) I-kappa B Kinase (日) (読) [継承]
12. (英) Inhibitor of Apoptosis Proteins (日) (読) [継承]
13. (英) Interleukin-6 (日) (読) [継承]
14. (英) Interleukin-8 (日) (読) [継承]
15. (英) Luciferases (日) (読) [継承]
16. (英) Mice (日) (読) [継承]
17. (英) Mice, Inbred BALB C (日) (読) [継承]
18. (英) Mice, Nude (日) (読) [継承]
19. (英) Mouth Neoplasms (日) (読) [継承]
20. (英) NF-kappa B (日) (読) [継承]
21. (英) RNA, Messenger (日) (読) [継承]
22. (英) Radiation, Ionizing (日) (読) [継承]
23. (英) Radiation-Protective Agents (日) (読) [継承]
発行所 (推奨):
誌名 (必須): International Journal of Oncology (International Center for Cancer Research)
(pISSN: 1019-6439, eISSN: 1791-2423)

ISSN (任意): 1019-6439
ISSN: 1019-6439 (pISSN: 1019-6439, eISSN: 1791-2423)
Title: International journal of oncology
Title(ISO): Int J Oncol
Publisher: Spandidos Publications
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2007年 10月 初日 (平成 19年 10月 初日) [継承]
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PMID (任意): 17786306    (→Scopusで検索) [継承]
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WOS (任意): 000249795300009 [継承]
Scopus (任意): 2-s2.0-35848957914 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Therapeutic Regulation for Oral Tumors, Institute of Health BioSciences, The University of Tokushima Graduate School of Dentistry, Tokushima city, Tokushima 770-8504, Japan. ttama@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Tetsuya Tamatani, Masayuki Azuma, Katsumi Motegi, Natsumi Takamaru, Yuichiro Kawashima and Takashi Bando : Cepharanthin-enhanced radiosensitivity through the inhibition of radiation-induced nuclear factor-kappaB activity in human oral squamous cell carcinoma cells., International Journal of Oncology, Vol.31, No.4, 761-768, 2007.
欧文冊子 ● Tetsuya Tamatani, Masayuki Azuma, Katsumi Motegi, Natsumi Takamaru, Yuichiro Kawashima and Takashi Bando : Cepharanthin-enhanced radiosensitivity through the inhibition of radiation-induced nuclear factor-kappaB activity in human oral squamous cell carcinoma cells., International Journal of Oncology, Vol.31, No.4, 761-768, 2007.

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