| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 永井 宏和
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| 2. | (英) Terada Kunihiko (日) (読)
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| 3. | (英) Watanabe Go (日) (読)
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| 4. | (英) Ueno Yasuharu (日) (読)
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| 5. | (英) Aiba Namiko (日) (読)
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| 6. | (英) Shibuya Tomomi (日) (読)
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| 7. | (英) Kawagoe Masami (日) (読)
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| 8. | (英) Kameda Takashi (日) (読)
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| 9. | (英) Sato Mitsuru (日) (読)
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| 10. | (英) Senoo Haruki (日) (読)
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| 11. | (英) Sugiyama Toshihiro (日) (読)
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| ○題名 (必須): | □ | (英) Differentiation of liver epithelial (stem-like) cells into hepatocytes induced by coculture with hepatic stellate cells. (日)
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| ○要約 (任意): | □ | (英) The liver is believed to contain stem cells that can differentiate into either hepatocytes or biliary epithelial cells. In the present study, we established a nonhepatocytic epithelial cell line from the normal livers of adult rats. The established cells, designated HSL cells, were immunoreactive against alpha-fetoprotein, but neither albumin nor cytokeratin 19. To demonstrate the differentiation potential of HSL cells in vitro, the cells were cocultured with hepatic stellate cells as a mixture or separately using insert wells. Consequently, although coculture with hepatic stellate cells rendered HSL cells able to produce albumin, the mixed coculture system mimicking the hepatic environment elicited this phenomenon more effectively than the separated coculture system. In conclusion, HSL cells have immature properties and the potential to differentiate into mature cells. Not only the extracellular matrices but also soluble factors, which are produced by hepatic stellate cells, induce this maturation, demonstrating the importance of the hepatic environment for hepatocyte differentiation. (日)
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| ○キーワード (推奨): | 1. | (英) Albumins (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Blotting, Western (日) (読)
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| 4. | 細胞分化 (cell differentiation)
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| 5. | (英) Coculture Techniques (日) (読)
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| 6. | (英) Epithelial Cells (日) (読)
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| 7. | (英) Hepatocytes (日) (読)
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| 8. | 免疫組織化学 (immunohistochemistry)
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| 9. | (英) Liver (日) (読)
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| 10. | (英) Male (日) (読)
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| 11. | (英) Microscopy, Fluorescence (日) (読)
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| 12. | (英) Microscopy, Phase-Contrast (日) (読)
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| 13. | (英) Phenotype (日) (読)
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| 14. | (英) Rats (日) (読)
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| 15. | (英) Rats, Sprague-Dawley (日) (読)
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| 16. | (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)
| ○ISSN (任意): | □ | 0006-291X
ISSN: 0006-291X
(pISSN: 0006-291X, eISSN: 1090-2104) Title: Biochemical and biophysical research communicationsTitle(ISO): Biochem Biophys Res CommunPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 293
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| ○号 (必須): | □ | 5
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| ○頁 (必須): | □ | 1420 1425
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2002年 5月 24日 (平成 14年 5月 24日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/S0006-291X(02)00406-0 (→Scopusで検索)
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| ○PMID (任意): | □ | 12054673 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): | □ | 2-s2.0-18444419136
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biochemistry, Akita University School of Medicine, Hondo 1-1-1, Akita 010-8543, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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