『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=22409)

EID=22409EID:22409, Map:0, LastModified:2018年8月27日(月) 21:58:04, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[宇都 義浩], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.工学部.生物工学科.生物機能工学講座 [継承]
著者 (必須): 1. (英) Takenana Shigeori (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Yamashita Kenichi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Takagi Makoto (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.宇都 義浩 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Kondo Hiroki (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) DNA Sensing on a DNA Probe-Modified Electrode Using Ferrocenylnaphthalene Diimide as the Electrochemically Active Ligand  (日)    [継承]
副題 (任意):
要約 (任意): (英) Naphthalene diimide derivative 1 carrying ferrocenyl moieties at the termini of imide substituents binds intact calf thymus DNA 4 times more strongly than the denatured DNA, and its complex with the intact DNA dissociates 80 times more slowly than that with the denatured DNA. On the basis of these observations, ligand 1 was applied to a probe of electrochemical DNA sensing. A thiol-linked single-stranded DNA probe was immobilized through the S-Au bonding to 20-30 pmol/mm2 on a gold electrode. Following hybridization with the complementary DNA, the electrode was soaked in a solution containing 1 (intercalation step) and then washed with buffer for 5 s. The cyclic voltammogram and differential pulse voltammogram for this electrode gave an electrochemical signal due to the redox reaction of 1 that was bound to the double-stranded DNA on the electrode. Thus, dA20 and the yeast choline transport gene were quantitated at the subpicomole level. The sensitivity of DNA detection was improved to 10 zmol by reducing the amount of immobilized DNA probe and protecting the uncovered surface of the electrode with 2-mercaptoethanol.  (日)    [継承]
キーワード (推奨): 1. (英) Base Sequence (日) (読) [継承]
2. (英) DNA (日) (読) [継承]
3. (英) DNA Probes (日) (読) [継承]
4. (英) Electrochemistry (日) (読) [継承]
5. (英) Electrodes (日) (読) [継承]
6. (英) Ferrous Compounds (日) (読) [継承]
7. (英) Imides (日) (読) [継承]
8. (英) Kinetics (日) (読) [継承]
9. (英) Ligands (日) (読) [継承]
10. (英) Magnetic Resonance Spectroscopy (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Analytical Chemistry ([アメリカ化学会])
(pISSN: 0003-2700, eISSN: 1520-6882)

ISSN (任意): 0003-2700
ISSN: 0003-2700 (pISSN: 0003-2700, eISSN: 1520-6882)
Title: Analytical chemistry
Title(ISO): Anal. Chem.
Publisher: American Chemical Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 72 [継承]
(必須): 6 [継承]
(必須): 1334 1341 [継承]
都市 (任意):
年月日 (必須): 西暦 2000年 5月 15日 (平成 12年 5月 15日) [継承]
URL (任意):
DOI (任意): 10.1021/ac991031j    (→Scopusで検索) [継承]
PMID (任意): 10740879    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000085975200034 [継承]
Scopus (任意): 2-s2.0-0034654434 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Shigeori Takenana, Kenichi Yamashita, Makoto Takagi, Yoshihiro Uto and Hiroki Kondo : DNA Sensing on a DNA Probe-Modified Electrode Using Ferrocenylnaphthalene Diimide as the Electrochemically Active Ligand, Analytical Chemistry, Vol.72, No.6, 1334-1341, 2000.
欧文冊子 ● Shigeori Takenana, Kenichi Yamashita, Makoto Takagi, Yoshihiro Uto and Hiroki Kondo : DNA Sensing on a DNA Probe-Modified Electrode Using Ferrocenylnaphthalene Diimide as the Electrochemically Active Ligand, Analytical Chemistry, Vol.72, No.6, 1334-1341, 2000.

関連情報

Number of session users = 1, LA = 0.45, Max(EID) = 361906, Max(EOID) = 968322.