『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=22372EID:22372, Map:0, LastModified:2015年9月20日(日) 19:33:33, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[宇都 義浩], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.京都大学 [継承]
著者 (必須): 1. (英) Masunaga Shin-ichiro (日) (読)
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2. (英) Ono Koji (日) (読)
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3. (英) Suzuki Minoru (日) (読)
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4. (英) Nishimura Yasumasa (日) (読)
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5. (英) Kinashi Yuko (日) (読)
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6. (英) Takagaki Masao (日) (読)
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7.堀 均
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8.永澤 秀子 (岐阜薬科大学/->個人[紺世 秀子])
役割 (任意): (英)   (日) TPZの合成を担当.   [継承]
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9.宇都 義浩 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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10. (英) Tsuchiya Izumi (日) (読)
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11. (英) Sadahiro Sotaro (日) (読)
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[継承]
12. (英) Murayama Chieko (日) (読)
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[継承]
題名 (必須): (英) Radiosensitization effect by combination with paclitaxel in vivo, including the effect on intratumor quiscent cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) To evaluate the radiosensitization effect on solid tumors upon combination treatment with paclitaxel (TXL), including the effect on intratumor quiescent (Q) cells. Mice bearing SCC VII or EL4 solid tumors received 5-bromo-2'-deoxyuridine (BrdU) continuously for 5 days to label all proliferating (P) cells. The mice then received gamma-irradiation with or without tirapazamine (TPZ) at various time points after TXL administration. Another group of mice received a series of test doses of gamma-rays while alive or after tumor clamping to obtain hypoxic fractions (HFs) in the tumors at various time points after TXL administration. Immediately after irradiation, the tumor cells were isolated and incubated with a cytokinesis blocker. The micronucleus (MN) frequency in cells without BrdU labeling (Q cells) was determined using immunofluorescence staining for BrdU. Meanwhile, 6 h after irradiation, the tumor cells were isolated from the solid tumors in another group of mice, and the apoptosis frequency in Q cells was also determined with immunofluorescence staining for BrdU. The MN and apoptosis frequency in total (P + Q) tumor cells were determined from the tumors that were not pretreated with BrdU. For the measurement of the HFs, the MN or apoptosis frequency of Q cells was then used to calculate the surviving fraction of Q cells from the regression line for the relationship between the MN or apoptosis frequency and the surviving fraction of total tumor cells. In both SCC VII and EL4 tumors, maximum values of mitotic index (MI) and apoptosis frequency were observed 9 and 24 h after TXL administration, respectively. However, on the whole, the apoptosis frequency for SCC VII was very low. gamma-Irradiation 9 h after TXL administration induced significant radiosensitization effects on the total cells of both tumors. Irradiation at 60 h had a more significant effect on total cells of EL4 tumor, but no significant effect on total cells of SCC VII tumor. Combined treatment with TXL induced no radiosensitization effect on Q cells in either tumor. The effect on Q cells was observed only after TPZ was administered. The HF of total cells in EL4 tumors decreased significantly 60 h after TXL administration. No radiosensitization effect upon combination treatment with TXL is induced in Q tumor cells. However, the effect on P cells is produced by irradiation at the time when the maximum values of MI are induced following TXL administration. In addition, for tumors that are susceptible to apoptosis after TXL administration alone, irradiation at the time of sufficient reoxygenation in tumors after TXL administration produces a greater radioenhancement effect on P cells.  (日) 本論文では,腫瘍移殖担癌マウスモデルにおいて,TPZ存在または非存在下での放射線処理にpaclitaxel(TXL)を併用した場合の影響について解析した.その結果,TXLはP細胞において増感作用を示すことを明らかにした.   [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Apoptosis (日) (読) [継承]
3. (英) Bromodeoxyuridine (日) (読) [継承]
4. (英) Carcinoma, Squamous Cell (日) (読) [継承]
5. (英) Cell Survival (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7. (英) Mice (日) (読) [継承]
8. (英) Mice, Inbred C3H (日) (読) [継承]
9. (英) Micronucleus Tests (日) (読) [継承]
10. (英) Neoplasms (日) (読) [継承]
11. (英) Paclitaxel (日) (読) [継承]
12. (英) Radiation Tolerance (日) (読) [継承]
13. (英) Radiation-Sensitizing Agents (日) (読) [継承]
14. (英) Radiobiology (日) (読) [継承]
15. (英) Radiotherapy Dosage (日) (読) [継承]
16. (英) Time Factors (日) (読) [継承]
17. (英) Triazines (日) (読) [継承]
発行所 (推奨): Elsevier Science [継承]
誌名 (必須): International Journal of Radiation Oncology*Biology*Physics ([Elsevier])
(pISSN: 0360-3016, eISSN: 1879-355X)

ISSN (任意): 0360-3016
ISSN: 0360-3016 (pISSN: 0360-3016, eISSN: 1879-355X)
Title: International journal of radiation oncology, biology, physics
Title(ISO): Int. J. Radiat. Oncol. Biol. Phys.
Publisher: Elsevier BV
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(必須): 50 [継承]
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(必須): 1063 1072 [継承]
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年月日 (必須): 西暦 2001年 7月 15日 (平成 13年 7月 15日) [継承]
URL (任意): http://dx.doi.org/10.1016/S0360-3016(01)01553-X [継承]
DOI (任意): 10.1016/S0360-3016(01)01553-X    (→Scopusで検索) [継承]
PMID (任意): 11429234    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Shin-ichiro Masunaga, Koji Ono, Minoru Suzuki, Yasumasa Nishimura, Yuko Kinashi, Masao Takagaki, Hitoshi Hori, Hideko Nagasawa, Yoshihiro Uto, Izumi Tsuchiya, Sotaro Sadahiro and Chieko Murayama : Radiosensitization effect by combination with paclitaxel in vivo, including the effect on intratumor quiscent cells, International Journal of Radiation Oncology*Biology*Physics, Vol.50, No.4, 1063-1072, 2001.
欧文冊子 ● Shin-ichiro Masunaga, Koji Ono, Minoru Suzuki, Yasumasa Nishimura, Yuko Kinashi, Masao Takagaki, Hitoshi Hori, Hideko Nagasawa, Yoshihiro Uto, Izumi Tsuchiya, Sotaro Sadahiro and Chieko Murayama : Radiosensitization effect by combination with paclitaxel in vivo, including the effect on intratumor quiscent cells, International Journal of Radiation Oncology*Biology*Physics, Vol.50, No.4, 1063-1072, 2001.

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