| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 山田 安希子
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| 2. | 山本 武範
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| 3. | 山﨑 尚志 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学]/[徳島大学.薬学部.創製薬科学科.創薬学講座.衛生薬学]/[徳島大学.薬学研究科.創薬科学専攻])
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| 4. | 山下 菊治
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| 5. | 片岡 正俊
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| 6. | 永田 俊彦
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| 7. | 寺田 弘 ([東京理科大学])
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| 8. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| ○題名 (必須): | □ | (英) Differential permeabilization effects of Ca2+ and valinomycin on the inner and outer mitochondrial membranes as revealed by proteomics analysis of proteins released from mitochondria. (日)
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| ○要約 (任意): | □ | (英) It is well established that cytochrome c is released from mitochondria when the permeability transition (PT) of this organelle is induced by Ca2+. Our previous study showed that valinomycin also caused the release of cytochrome c from mitochondria but without inducing this PT (Shinohara, Y., Almofti, M. R., Yamamoto, T., Ishida, T., Kita, F., Kanzaki, H., Ohnishi, M., Yamashita, K., Shimizu, S., and Terada, H. (2002) Permeability transition-independent release of mitochondrial cytochrome c induced by valinomycin. Eur. J. Biochem. 269, 5224-5230). These results indicate that cytochrome c may be released from mitochondria with or without the induction of PT. In the present study, we examined the protein species released from valinomycin- and Ca2+-treated mitochondria by LC-MS/MS analysis. As a result, the proteins located in the intermembrane space were found to be specifically released from valinomycin-treated mitochondria, whereas those in the intermembrane space and in the matrix were released from Ca2+-treated mitochondria. These results were confirmed by Western analysis. Furthermore to examine how the protein release occurred, we examined the correlation between the species of released proteins and those of the abundant proteins in mitochondria. Consequently most of the proteins released from mitochondria treated with either agent were highly expressed proteins in mitochondria, indicating that the release occurred not selectively but in a manner dependent on the concentration of the proteins. Based on these results, the permeabilization effects of Ca2+ and valinomycin on the inner and outer mitochondrial membranes are discussed. (日)
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| ○キーワード (推奨): | 1. | (英) Amino Acid Sequence (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Blotting, Western (日) (読)
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| 4. | (英) Calcium (日) (読)
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| 5. | (英) Cell Membrane Permeability (日) (読)
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| 6. | (英) Electrophoresis, Polyacrylamide Gel (日) (読)
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| 7. | (英) Intracellular Membranes (日) (読)
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| 8. | (英) Microscopy, Electron, Transmission (日) (読)
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| 9. | (英) Mitochondria, Liver (日) (読)
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| 10. | (英) Molecular Sequence Data (日) (読)
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| 11. | (英) Proteins (日) (読)
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| 12. | (英) Proteomics (日) (読)
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| 13. | (英) Rats (日) (読)
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| 14. | (英) Valinomycin (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Molecular & Cellular Proteomics ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 1535-9476, eISSN: 1535-9484)
| ○ISSN (任意): | □ | 1535-9484
ISSN: 1535-9476
(pISSN: 1535-9476, eISSN: 1535-9484) Title: Molecular & cellular proteomics : MCPTitle(ISO): Mol Cell ProteomicsPublisher: American Society for Biochemistry and Molecular Biology (NLM Catalog)
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(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 8
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| ○号 (必須): | □ | 6
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| ○頁 (必須): | □ | 1265 1277
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| ○年月日 (必須): | □ | 西暦 2009年 2月 14日 (平成 21年 2月 14日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1074/mcp.M800377-MCP200 (→Scopusで検索)
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| ○PMID (任意): | □ | 19218587 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: double daggerSchool of Dentistry, University of Tokushima, Kuramotocho-3, Tokushima 770-8504, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) OtherID: PMC2690487 (日)
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