| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Akamine Rie (日) (読)
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| 2. | 山本 武範
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| 3. | (英) Watanabe Masahiro (日) (読)
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| 4. | 山﨑 尚志 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学]/[徳島大学.薬学部.創製薬科学科.創薬学講座.衛生薬学]/[徳島大学.薬学研究科.創薬科学専攻])
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| 5. | 片岡 正俊
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| 6. | 石川 満 ([独立行政法人 産業技術総合研究所])
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| 7. | 大家 利彦 (産業技術総合研究所健康工学研究センター)
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| 8. | 馬場 嘉信
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| 9. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| ○題名 (必須): | □ | (英) Usefulness of the 5' region of the cDNA encoding acidic ribosomal phosphoprotein P0 conserved among rats, mice, and humans as a standard probe for gene expression analysis in different tissues and animal species. (日)
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| ○要約 (任意): | □ | (英) Housekeeping genes are often used as internal standards for gene expression analysis. When steady-state transcript levels of 4 typically used housekeeping genes, i.e., beta-actin, glyceraldehyde 3-phosphate dehydrogenase, cyclophilin, and acidic ribosomal phosphoprotein P0 (36B4), were evaluated in various rat tissues, the 36B4 gene seemed to be the most suitable as a standard to compare the expression levels of genes among different tissues. Next, for possible quantitative comparison of the expression level of this gene among different animal species, we compared the nucleotide sequence of the cDNA of 36B4 among rats, mice, and humans. As a result, highly conserved regions showing more than 97.5% identities were observed in the 5' portion of its open reading frame. When samples of synthesized mRNA encoding rat, mouse, and human 36B4 were hybridized with the entire cDNA encoding rat 36B4 as a probe, hybridization signals of mRNAs of mouse and human 36B4 were much weaker than those of mRNA encoding rat 36B4. However, when they were hybridized with an oligonucleotide probe corresponding to the highly conserved regions, they showed similar signal intensities. Thus, these highly conserved regions of the cDNA encoding 36B4 were concluded to be an effective standard for use in gene expression analysis. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Base Sequence (日) (読)
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| 3. | (英) Conserved Sequence (日) (読)
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| 4. | (英) DNA, Complementary (日) (読)
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| 5. | (英) Gene Expression Profiling (日) (読)
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| 6. | (英) Humans (日) (読)
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| 7. | (英) Mice (日) (読)
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| 8. | (英) Molecular Sequence Data (日) (読)
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| 9. | (英) Phosphoproteins (日) (読)
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| 10. | (英) RNA, Messenger (日) (読)
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| 11. | (英) Rats (日) (読)
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| 12. | (英) Ribosomal Proteins (日) (読)
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| 13. | (英) Sequence Homology, Nucleic Acid (日) (読)
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| 14. | (英) Species Specificity (日) (読)
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| 15. | (英) Tissue Distribution (日) (読)
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| 16. | (英) Transcription, Genetic (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Journal of Biochemical and Biophysical Methods ([Elsevier])
(pISSN: 0165-022X)
| ○ISSN (任意): | □ | 0165-022X
ISSN: 0165-022X
(pISSN: 0165-022X, eISSN: 1872-857X) Title: Journal of biochemical and biophysical methodsTitle(ISO): J Biochem Biophys MethodsPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 70
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 481 486
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2007年 4月 10日 (平成 19年 4月 10日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.jbbm.2006.11.008 (→Scopusで検索)
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| ○PMID (任意): | □ | 17196660 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Institute for Genome Research, Faculty of Pharmaceutical Sciences, University of Tokushima, Kuramotocho-3, Tokushima 770-8503, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Comparative Study (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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