『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=221462EID:221462, Map:0, LastModified:2013年6月7日(金) 15:09:53, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[山﨑 尚志], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hirano Ken (日) (読)
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2. (英) Yoshida Yuichiro (日) (読)
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3. (英) Ishido Tomomi (日) (読)
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4. (英) Wada Yukihisa (日) (読)
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5. (英) Moriya Naoji (日) (読)
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6.山﨑 尚志 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.薬物治療学])
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7. (英) Mizushina Yoshiyuki (日) (読)
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8.馬場 嘉信
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9.石川 満 ([独立行政法人 産業技術総合研究所])
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題名 (必須): (英) Consecutive incorporation of fluorophore-labeled nucleotides by mammalian DNA polymerase beta  (日)    [継承]
副題 (任意):
要約 (任意): (英) In the present study, we investigated mammalian polymerases that consecutively incorporate various fluorophore-labeled nucleotides. We found that rat DNA polymerase beta (pol beta) consecutively incorporated fluorophore-labeled nucleotides to a greater extent than four bacterial polymerases, Sequenase Version 2.0, Vent(R) (exo-), DNA polymerase IIIalpha and the Klenow fragment, and the mammalian polymerases DNA polymerase alpha and human DNA polymerase delta, under mesophilic conditions. Furthermore, we investigated the kinetics of correct or mismatched incorporation with labeled nucleotides during synthesis by rat pol beta. The kinetic parameters K(m) and k(cat) were measured and used for evaluating: (i) the discrimination against correct pair incorporation of labeled nucleotides relative to unlabeled nucleotides; and (ii) the fidelity for all nucleotide combinations of mismatched pairs in the presence of labeled or unlabeled nucleotides. We also investigated the effect of fluorophore-labeled nucleotides on terminal deoxynucleotidyl transferase activity of rat pol beta. We have demonstrated for the first time that mammalian pol beta can consecutively incorporate various fluorophore-labeled dNTPs. These findings suggest that pol beta is useful for high-density labeling of DNA probes and single-molecule sequencing for high-speed genome analysis.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) DNA Nucleotidylexotransferase (日) (読) [継承]
3. (英) DNA Polymerase III (日) (読) [継承]
4. (英) DNA Polymerase beta (日) (読) [継承]
5. (英) DNA Probes (日) (読) [継承]
6. (英) Fluorescein (日) (読) [継承]
7. (英) Fluorescent Dyes (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Nucleotides (日) (読) [継承]
10. (英) Rats (日) (読) [継承]
発行所 (推奨): Elsevier (->組織[Elsevier Science]) [継承]
誌名 (必須): Analytical Biochemistry: Methods in the Biological Sciences ([Elsevier Science])
(pISSN: 0003-2697, eISSN: 1096-0309)

ISSN (任意): 1096-0309
ISSN: 0003-2697 (pISSN: 0003-2697, eISSN: 1096-0309)
Title: Analytical biochemistry
Title(ISO): Anal Biochem
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 405 [継承]
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年月日 (必須): 西暦 2010年 10月 初日 (平成 22年 10月 初日) [継承]
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DOI (任意): 10.1016/j.ab.2010.06.005    (→Scopusで検索) [継承]
PMID (任意): 20570644    (→Scopusで検索) [継承]
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WOS (任意): 000281296300003 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Nano-Bioanalysis Team, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, Japan. hirano-ken@aist.go.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Ken Hirano, Yuichiro Yoshida, Tomomi Ishido, Yukihisa Wada, Naoji Moriya, Naoshi Yamazaki, Yoshiyuki Mizushina, Yoshinobu Baba and Mitsuru Ishikawa : Consecutive incorporation of fluorophore-labeled nucleotides by mammalian DNA polymerase beta, Analytical Biochemistry: Methods in the Biological Sciences, Vol.405, No.2, 160-167, 2010.
欧文冊子 ● Ken Hirano, Yuichiro Yoshida, Tomomi Ishido, Yukihisa Wada, Naoji Moriya, Naoshi Yamazaki, Yoshiyuki Mizushina, Yoshinobu Baba and Mitsuru Ishikawa : Consecutive incorporation of fluorophore-labeled nucleotides by mammalian DNA polymerase beta, Analytical Biochemistry: Methods in the Biological Sciences, Vol.405, No.2, 160-167, 2010.

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