『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=219834EID:219834, Map:0, LastModified:2017年12月16日(土) 22:20:52, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[細井 和雄], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座.口腔分子生理学 (2004年4月1日〜2015年3月31日) [継承]
著者 (必須): 1. (英) Karabasil, Ratko, Mileva (日) Karabasil, Ratko, Mileva (読)
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2.長谷川 敬展 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
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3. (英) Azlina, Ahmad (日) Azlina, Ahmad (読)
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4. (英) Purwanti, Nunuk (日) Purwanti, Nunuk (読)
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5.姚 陳娟 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
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6.赤松 徹也 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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7.富岡 重正
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8.細井 和雄
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題名 (必須): (英) Effects of naturally occurring G103D point mutation of AQP5 on its water permeability, trafficking, and cellular localization in the submandibular gland of rats  (日)    [継承]
副題 (任意):
要約 (任意): (英) AQPs (aquaporins) are water channel proteins that are expressed in almost all living things. In mammalians, 13 members of AQPs (AQP0-12) have been identified so far. AQP5 is known to be expressed mostly in the exocrine cells, including the salivary gland acinar cells. A naturally occurring point mutation (G308A, Gly103 > Asp103) was earlier found in the rat AQP5 gene [Murdiastuti, Purwanti, Karabasil, Li, Yao, Akamatsu, Kanamori and Hosoi (2006) Am. J. Physiol. 291, G1081-G1088]; in this mutant, the rate of initial saliva secretion under stimulated and unstimulated conditions is less than that for the wt (wild-type) animals. Here the mutant molecule was characterized in detail. Using the Xenopus oocyte system, we demonstrated the mutant AQP5 to have water permeability almost the same as that of the wt molecule. Mutant and wt AQP5s, tagged with GFP (green fluorescent protein; GFP-AQP5s) and expressed in polarized MDCK-II (Madin-Darby canine kidney II) cells, first appeared in the vesicular structure(s) in the cytoplasm, and were translocated to the upper plasma membrane or apical membrane during cultivation, with the mutant GFP-AQP5 being translocated less efficiently. Thapsigargin and H-89 both induced translocation in vitro of either molecule, whereas colchicine inhibited this activity; the fraction of cells showing apical localization of mutant GFP-AQP5 was less than that showing that of the wt molecule under any of the experimental conditions used. In the mutant SMG (submandibular gland) tissue, localization of AQP5 in the apical membrane of acinar cells was extremely reduced. Vesicular structures positive for AQP5 and present in the cytoplasm of the acinar cells were co-localized with LAMP2 (lysosome-associated membrane protein 2) or cathepsin D in the mutant gland, whereas such co-localizations were very rare in the wt gland, suggesting that the mutant molecules largely entered lysosomes for degradation. Replacement of highly conserved hydrophobic Gly103 with strongly hydrophilic Asp103 in rat AQP5, though it did not affect water permeability, may possibly have resulted in less efficient membrane trafficking and increased lysosomal degradation, leading to its lower expression in the apical membrane of the acinar cells in the SMG.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Aquaporin 5 (日) (読) [継承]
4. (英) Cell Line (日) (読) [継承]
5. (英) Cell Membrane (日) (読) [継承]
6. (英) Dogs (日) (読) [継承]
7. (英) Molecular Sequence Data (日) (読) [継承]
8. (英) Mutation, Missense (日) (読) [継承]
9. (英) Permeability (日) (読) [継承]
10. (英) Point Mutation (日) (読) [継承]
11. (英) Protein Transport (日) (読) [継承]
12. (英) Rats (日) (読) [継承]
13. (英) Sequence Alignment (日) (読) [継承]
14. (英) Submandibular Gland (日) (読) [継承]
15. (英) Water (日) (読) [継承]
16. (英) Xenopus (日) (読) [継承]
発行所 (推奨): Portland Press [継承]
誌名 (必須): Biology of the Cell (Société française de microscopie électronique/European Cell Biology Organization/Centre national de la recherche scientifique/Institut national de la santé et de la recherche médicale/Société française des microscopies/Société de biologie cellulaire de France)
(pISSN: 0248-4900, eISSN: 1768-322X)

ISSN (任意): 1768-322X
ISSN: 0248-4900 (pISSN: 0248-4900, eISSN: 1768-322X)
Title: Biology of the cell
Title(ISO): Biol Cell
Supplier: Société Française des Microscopies and Société Biologie Cellulaire de France
Publisher: Portland Press
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年月日 (必須): 西暦 2011年 1月 初日 (平成 23年 1月 初日) [継承]
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DOI (任意): 10.1042/BC20100086    (→Scopusで検索) [継承]
PMID (任意): 21138418    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Molecular Oral Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima-shi, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Mileva Ratko Karabasil, Takahiro Hasegawa, Ahmad Azlina, Nunuk Purwanti, Chenjuan Yao, Tetsuya Akamatsu, Shigemasa Tomioka and Kazuo Hosoi : Effects of naturally occurring G103D point mutation of AQP5 on its water permeability, trafficking, and cellular localization in the submandibular gland of rats, Biology of the Cell, Vol.103, No.2, 69-86, 2011.
欧文冊子 ● Mileva Ratko Karabasil, Takahiro Hasegawa, Ahmad Azlina, Nunuk Purwanti, Chenjuan Yao, Tetsuya Akamatsu, Shigemasa Tomioka and Kazuo Hosoi : Effects of naturally occurring G103D point mutation of AQP5 on its water permeability, trafficking, and cellular localization in the submandibular gland of rats, Biology of the Cell, Vol.103, No.2, 69-86, 2011.

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