『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=215308EID:215308, Map:0, LastModified:2012年8月29日(水) 12:20:45, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[野間 隆文], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学 [継承]
2.山口大学 [継承]
著者 (必須): 1. (英) Fujisawa Koichi (日) (読)
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2. (英) Murakami Ryutaro (日) (読)
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3.堀口 大吾 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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[継承]
4.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Adenylate kinase isozyme 2 is essential for growth and development of Drosophila melanogaster.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Adenylate kinases are phylogenetically widespread, highly conserved, and involved in energy metabolism and energy transfer. Of these, adenylate kinase (AK) isozyme 2 is uniquely localized in the mitochondrial intermembrane space and its physiological role remains largely unknown. In this study, we selected Drosophila melanogaster to analyze its role in vivo. AK isozyme cDNAs were cloned and their gene expressions were characterized in D. melanogaster. The deduced amino acid sequences contain highly conserved motifs for P-loop, NMP binding, and LID domains of AKs. In addition, the effects of AK2 gene knockout on phenotype of AK2 mutants were examined using P-element technology. Although homozygous AK2 mutated embryos developed without any visible defects, their growth ceased and they died before reaching the third instar larval stage. Maternally provided AK2 mRNA was detected in fertilized eggs, and weak AK2 activity was observed in first and second instar larvae of the homozygous AK2 mutants, suggesting that maternally provided AK2 is sufficient for embryonic development. Disappearance of AK2 activity during larval stages resulted in growth arrest and eventual death. These results demonstrate that AK2 plays a critical role in adenine nucleotide metabolism in the mitochondrial intermembrane space and is essential for growth in D. melanogaster.  (日)    [継承]
キーワード (推奨): 1. (英) Adenine Nucleotides (日) (読) [継承]
2. (英) Adenylate Kinase (日) (読) [継承]
3. (英) Amino Acid Sequence (日) (読) [継承]
4. (英) Animals (日) (読) [継承]
5. (英) Blotting, Northern (日) (読) [継承]
6. (英) Blotting, Western (日) (読) [継承]
7. (英) Cloning, Molecular (日) (読) [継承]
8. (英) Drosophila Proteins (日) (読) [継承]
9. (英) Drosophila melanogaster (日) (読) [継承]
10. (英) Enzyme Assays (日) (読) [継承]
11. (英) Female (日) (読) [継承]
12. (英) Gene Expression Profiling (日) (読) [継承]
13. (英) Gene Expression Regulation, Developmental (日) (読) [継承]
14. (英) Gene Expression Regulation, Enzymologic (日) (読) [継承]
15. (英) Kinetics (日) (読) [継承]
16. (英) Male (日) (読) [継承]
17. (英) Molecular Sequence Data (日) (読) [継承]
18. (英) Mutation (日) (読) [継承]
19. (英) Phylogeny (日) (読) [継承]
20. (英) Sequence Homology, Amino Acid (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology ([Elsevier])
(pISSN: 1096-4959, eISSN: 1879-1107)

ISSN (任意): 1879-1107
ISSN: 1096-4959 (pISSN: 1096-4959, eISSN: 1879-1107)
Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
Title(ISO): Comp. Biochem. Physiol. B, Biochem. Mol. Biol.
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2009年 1月 19日 (平成 21年 1月 19日) [継承]
URL (任意):
DOI (任意): 10.1016/j.cbpb.2009.01.006    (→Scopusで検索) [継承]
PMID (任意): 19416704    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Affiliation: Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan.  (日)    [継承]
2.(英) PublicationType: Journal Article  (日)    [継承]
3.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Koichi Fujisawa, Ryutaro Murakami, Taigo Horiguchi and Takafumi Noma : Adenylate kinase isozyme 2 is essential for growth and development of Drosophila melanogaster., Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, Vol.153, No.1, 29-38, 2009.
欧文冊子 ● Koichi Fujisawa, Ryutaro Murakami, Taigo Horiguchi and Takafumi Noma : Adenylate kinase isozyme 2 is essential for growth and development of Drosophila melanogaster., Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, Vol.153, No.1, 29-38, 2009.

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