『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨): 単独著作 (徳島大学内の単一の研究グループ(研究室等)内の研究 (単著も含む)) [継承]
学究種別 (推奨): 博士課程学生による研究報告 [継承]
組織 (推奨): 1.疾患酵素学研究センター (2007年4月1日〜2016年3月31日/->組織[徳島大学.先端酵素学研究所.次世代酵素学研究領域]) [継承]
著者 (必須): 1.チャン ホアンナム ([徳島大学.高等教育研究センター.学修支援部門]/[徳島大学.国際センター])
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2.坂井 隆志
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3. (英) Kim Mi Sun (日) 金 善美 (読) きむ そんみ
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4.福井 清 ([徳島大学])
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題名 (必須): (英) NF-B regulates the expression of Nucling, a novel apoptosis regulator, with involvement of proteasome and caspase for its degradation  (日)    [継承]
副題 (任意):
要約 (任意): (英) Nucling is identified as a novel regulator of apoptosis. In this study, we investigated the nuclear factor-B (NF-B)-dependent mechanism of Nucling expression, as well as the degradation pathways mediated by proteasome system and caspase activation. Using chromatin immunoprecipitation assay in wild-type mouse embryonic fibroblasts (WT MEFs), we found that NF-B p65 could bind to the B motifs in the promoter regions of Nucling gene at four putative-binding sites. By real-time PCR and immunoblot, we confirmed that Nucling expression was up-regulated by tumour necrosis factor- (TNF-) stimulation in WT MEFs, but not in NF-B p50 knockout MEFs. On the other hand, we investigated the degradation of Nucling in connection with proteasome and caspase by using cycloheximide chase. The results showed that Nucling is a short-lived protein, and its degradation was recovered by proteasome inhibitor MG132. Moreover, under TNF- stimulation, degradation of Nucling was recovered by pan-caspase inhibitor zVAD-fmk. Taken together, we propose a mechanism of Nucling intracellular metabolism. Nucling expression is induced by canonical NF-B signalling pathway, whereas Nucling is undergoing proteasome degradation, as well as being cleaved by caspase system under stress conditions. This opens a new perspective for studying the NF-B dependent regulation mechanism of cell death and survival.  (日)    [継承]
キーワード (推奨): 1.アポトーシス (apoptosis) [継承]
2.カスパーゼ (caspase) [継承]
3.nuclear factor κB (nuclear factor κB) [継承]
4.ヌクリング (nucling) [継承]
5.tumor necrosis factor-α (tumor necrosis factor-α) [継承]
6.プロテアソーム (proteasome) [継承]
発行所 (推奨): Oxford University Press [継承]
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 1756-2651
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J. Biochem.
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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都市 (任意): オックスフォード (Oxford/[連合王国]) [継承]
年月日 (必須): 西暦 2010年 11月 1日 (平成 22年 11月 1日) [継承]
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DOI (任意): 10.1093/jb/mvq089    (→Scopusで検索) [継承]
PMID (任意): 20702393    (→Scopusで検索) [継承]
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WOS (任意): 000283689000007 [継承]
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備考 (任意): 1.(英) Article.Affiliation: The Institute for Enzyme Research, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● HoangNam Tran, Takashi Sakai, Sun Mi Kim and Kiyoshi Fukui : NF-B regulates the expression of Nucling, a novel apoptosis regulator, with involvement of proteasome and caspase for its degradation, The Journal of Biochemistry, Vol.148, No.5, 573-580, 2010.
欧文冊子 ● HoangNam Tran, Takashi Sakai, Sun Mi Kim and Kiyoshi Fukui : NF-B regulates the expression of Nucling, a novel apoptosis regulator, with involvement of proteasome and caspase for its degradation, The Journal of Biochemistry, Vol.148, No.5, 573-580, 2010.

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