『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨): 博士後期課程学生による研究報告 [継承]
組織 (推奨):
著者 (必須): 1. (英) Azuma-Mukai Asuka (日) (読)
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2. (英) Oguri Hideo (日) (読)
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3. (英) Mituyama Toutai (日) (読)
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4.銭 志栄
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5. (英) Asai Kiyoshi (日) (読)
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6.塩見 春彦
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7. (英) Siomi Mikiko C (日) (読)
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題名 (必須): (英) Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Small RNAs triggering RNA silencing are loaded onto Argonautes and then sequence-specifically guide them to target transcripts. Epitope-tagged human Argonautes (hAgo1, hAgo2, hAgo3, and hAgo4) are associated with siRNAs and miRNAs, but only epitope-tagged hAgo2 has been shown to have Slicer activity. Contrarily, how endogenous hAgos behave with respect to small RNA association and target RNA destruction has remained unclear. Here, we produced monoclonal antibodies for individual hAgos. High-throughput pyrosequencing revealed that immunopurified endogenous hAgo2 and hAgo3 associated mostly with miRNAs. Endogenous hAgo3 did not show Slicer function but localized in P-bodies, suggesting that hAgo3 endogenously expressed is, like hAgo2, involved in the miRNA pathway but antagonizes the RNAi activity of hAgo2. Sequence variations of miRNAs were found at both 5' and 3' ends, suggesting that multiple mature miRNAs containing different "seed" sequences can arise from one miRNA precursor. The hAgo antibodies we raised are valuable tools for ascertaining the functional behavior of endogenous Argonautes and miRNAs in RNA silencing.  (日)    [継承]
キーワード (推奨): 1. (英) Antibodies, Monoclonal (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Eukaryotic Initiation Factor-2 (日) (読) [継承]
4. (英) Fluorescent Antibody Technique (日) (読) [継承]
5. (英) HeLa Cells (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7. (英) Immunoprecipitation (日) (読) [継承]
8. (英) Jurkat Cells (日) (読) [継承]
9. (英) MicroRNAs (日) (読) [継承]
10. (英) Mutation (日) (読) [継承]
11. (英) RNA Interference (日) (読) [継承]
12. (英) RNA, Small Interfering (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Proceedings of the National Academy of Sciences of the United States of America ([The National Academy of Sciences of the United States of America])
(pISSN: 0027-8424, eISSN: 1091-6490)

ISSN (任意): 1091-6490
ISSN: 0027-8424 (pISSN: 0027-8424, eISSN: 1091-6490)
Title: Proceedings of the National Academy of Sciences of the United States of America
Title(ISO): Proc Natl Acad Sci U S A
Publisher: National Academy of Sciences
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 105 [継承]
(必須): 23 [継承]
(必須): 7964 7969 [継承]
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年月日 (必須): 西暦 2008年 6月 4日 (平成 20年 6月 4日) [継承]
URL (任意):
DOI (任意): 10.1073/pnas.0800334105    (→Scopusで検索) [継承]
PMID (任意): 18524951    (→Scopusで検索) [継承]
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WOS (任意): 000256781800013 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Institute for Genome Research, University of Tokushima, Tokushima 770-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC2430345  (日)    [継承]

標準的な表示

和文冊子 ● Asuka Azuma-Mukai, Hideo Oguri, Toutai Mituyama, Zhi-Rong Qian, Kiyoshi Asai, Haruhiko Siomi and C Mikiko Siomi : Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing., Proceedings of the National Academy of Sciences of the United States of America, Vol.105, No.23, 7964-7969, 2008.
欧文冊子 ● Asuka Azuma-Mukai, Hideo Oguri, Toutai Mituyama, Zhi-Rong Qian, Kiyoshi Asai, Haruhiko Siomi and C Mikiko Siomi : Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing., Proceedings of the National Academy of Sciences of the United States of America, Vol.105, No.23, 7964-7969, 2008.

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