| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Umemoto Yoshihiro (日) (読)
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| 2. | 片岡 正俊
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| 3. | (英) Yatsushiro Shouki (日) (読)
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| 4. | (英) Watanabe Masahiro (日) (読)
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| 5. | 木戸 淳一
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| 6. | (英) Kakuhata Rei (日) (読)
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| 7. | 山本 武範
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| 8. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 9. | 馬場 嘉信
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| ○題名 (必須): | □ | (英) Sequential analysis of RNA synthesis by microchip electrophoresis. (日)
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| ○要約 (任意): | □ | (英) We describe the potential of microchip electrophoresis with a Hitachi SV1100, which can be used to evaluate the integrity of total RNA, for the analysis of synthesized RNA. There was little interference by DNA and/or the components of the in vitro transcription system with the microchip electrophoresis. The fluorescence intensity corresponding to the synthesized RNA increased in a time-dependent manner as to the RNA synthesis reaction on sequential analysis. A result can be obtained in 160 s and only 1/10 aliquots of samples, compared with the conventional method, are required. These results indicate the potential of microchip electrophoresis for sequential analysis of RNA synthesis. (日)
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| ○キーワード (推奨): | 1. | (英) Electrophoresis, Microchip (日) (読)
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| 2. | (英) Fluorescent Dyes (日) (読)
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| 3. | (英) RNA (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Analytical Biochemistry: Methods in the Biological Sciences ([Elsevier Science])
(pISSN: 0003-2697, eISSN: 1096-0309)
| ○ISSN (任意): | □ | 1096-0309
ISSN: 0003-2697
(pISSN: 0003-2697, eISSN: 1096-0309) Title: Analytical biochemistryTitle(ISO): Anal BiochemPublisher: Academic Press Inc. (NLM Catalog)
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| ○巻 (必須): | □ | 388
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 161 163
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2009年 2月 3日 (平成 21年 2月 3日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.ab.2009.01.040 (→Scopusで検索)
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| ○PMID (任意): | □ | 19454233 (→Scopusで検索)
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| ○WOS : | □ | 000265229200024
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, University of Tokushima, Shomachi 1-78, Tokushima 770-8505, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Technical Report (日)
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