| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Umemoto Yoshihiro (日) (読)
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| 2. | 片岡 正俊
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| 3. | (英) Yatsushiro Shouki (日) (読)
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| 4. | (英) Yamamura Shouhei (日) (読)
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| 5. | 大家 利彦 (産業技術総合研究所健康工学研究センター)
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| 6. | 木戸 淳一
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| 7. | 山本 武範
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| 8. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 9. | 馬場 嘉信
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| ○題名 (必須): | □ | (英) Analysis of DNA ligation by microchip electrophoresis. (日)
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| ○要約 (任意): | □ | (英) We describe the potential of microchip electrophoresis with a Hitachi SV1100, which can be used to determine DNA sizes between 500 and 5000 bp with good quantification (DNA concentration, <8 ng/l) within 5 min, for the analysis of DNA ligation. On analysis of an electropherogram of a ligation mixture of the pTAC1-T vector and a 789 bp PCR-amplified DNA fragment, the presence of recombinant DNA was easily detected by comparison with an electropherogram obtained without ligase. On analysis of a ligation mixture of pUC19/Eco RI without alkaline phosphatase treatment and a 667 bp Eco RI-digested fragment of foreign DNA, several peaks observed in the electropherogram corresponded to the formation of monomeric and polymeric insert DNAs, self-ligated vector DNA, and recombinant DNA. On the other hand, several peaks were also observed in the electropherogram of the ligation mixture of pUC19/Eco RI with alkaline phosphatase treatment and the 667 bp Eco RI-digested fragment of foreign DNA, the fluorescence intensity corresponding to recombinant DNA apparently being increased. These results indicate the potential of microchip electrophoresis for the analysis of DNA ligation, it offering high resolution in a short time. (日)
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| ○キーワード (推奨): | 1. | (英) Bacteriophage T4 (日) (読)
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| 2. | (英) Base Sequence (日) (読)
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| 3. | (英) Buffers (日) (読)
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| 4. | (英) Cloning, Molecular (日) (読)
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| 5. | (英) DNA Restriction Enzymes (日) (読)
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| 6. | (英) DNA, Circular (日) (読)
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| 7. | (英) DNA, Recombinant (日) (読)
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| 8. | (英) DNA, Viral (日) (読)
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| 9. | (英) Electrophoresis, Microchip (日) (読)
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| 10. | (英) Feasibility Studies (日) (読)
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| 11. | (英) Microfluidic Analytical Techniques (日) (読)
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| 12. | (英) Molecular Sequence Data (日) (読)
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| 13. | (英) Reference Standards (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Journal of Pharmaceutical and Biomedical Analysis (American Association of Pharmaceutical Scientists)
(pISSN: 0731-7085, eISSN: 1873-264X)
| ○ISSN (任意): | □ | 1873-264X
ISSN: 0731-7085
(pISSN: 0731-7085, eISSN: 1873-264X) Title: Journal of pharmaceutical and biomedical analysisTitle(ISO): J Pharm Biomed AnalPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 52
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 323 328
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2010年 6月 5日 (平成 22年 6月 5日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.jpba.2009.12.023 (→Scopusで検索)
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| ○PMID (任意): | □ | 20196235 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000274979700025
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, University of Tokushima, Shomachi 1-78, Tokushima 770-8505, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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