『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Ushizawa Koichi (日) (読)
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2. (英) Herath B. Chandana (日) (読)
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3. (英) Kaneyama Kanako (日) (読)
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4. (英) Shiojima Satoshi (日) (読)
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5. (英) Hirasawa Akira (日) (読)
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6. (英) Takahashi Toru (日) (読)
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7. (英) Imai Kei (日) (読)
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8.落合 和彦
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9. (英) Tokunaga Tomoyuki (日) (読)
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10. (英) Tsunoda Yukio (日) (読)
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11. (英) Tsujimoto Gozoh (日) (読)
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12. (英) Hashizume Kazuyoshi (日) (読)
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題名 (必須): (英) cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period.  (日)    [継承]
副題 (任意):
要約 (任意): (英) BACKGROUND: After fertilization, embryo development involves differentiation, as well as development of the fetal body and extra-embryonic tissues until the moment of implantation. During this period various cellular and molecular changes take place with a genetic origin, e.g. the elongation of embryonic tissues, cell-cell contact between the mother and the embryo and placentation. To identify genetic profiles and search for new candidate molecules involved during this period, embryonic gene expression was analyzed with a custom designed utero-placental complementary DNA (cDNA) microarray. METHODS: Bovine embryos on days 7, 14 and 21, extra-embryonic membranes on day 28 and fetuses on days 28 were collected to represent early embryo, elongating embryo, pre-implantation embryo, post-implantation extra-embryonic membrane and fetus, respectively. Gene expression at these different time points was analyzed using our cDNA microarray. Two clustering algorithms such as k-means and hierarchical clustering methods identified the expression patterns of differentially expressed genes across pre-implantation period. Novel candidate genes were confirmed by real-time RT-PCR. RESULTS: In total, 1,773 individual genes were analyzed by complete k-means clustering. Comparison of day 7 and day 14 revealed most genes increased during this period, and a small number of genes exhibiting altered expression decreased as gestation progressed. Clustering analysis demonstrated that trophoblast-cell-specific molecules such as placental lactogens (PLs), prolactin-related proteins (PRPs), interferon-tau, and adhesion molecules apparently all play pivotal roles in the preparation needed for implantation, since their expression was remarkably enhanced during the pre-implantation period. The hierarchical clustering analysis and RT-PCR data revealed new functional roles for certain known genes (dickkopf-1, NPM, etc) as well as novel candidate genes (AW464053, AW465434, AW462349, AW485575) related to already established trophoblast-specific genes such as PLs and PRPs. CONCLUSIONS: A large number of genes in extra-embryonic membrane increased up to implantation and these profiles provide information fundamental to an understanding of extra-embryonic membrane differentiation and development. Genes in significant expression suggest novel molecules in trophoblast differentiation.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cattle (日) (読) [継承]
3. (英) Cluster Analysis (日) (読) [継承]
4. (英) DNA, Complementary (日) (読) [継承]
5. (英) Embryo, Mammalian (日) (読) [継承]
6. (英) Embryonic Development (日) (読) [継承]
7. (英) Female (日) (読) [継承]
8. (英) Fetus (日) (読) [継承]
9. (英) Gene Expression Profiling (日) (読) [継承]
10. (英) Gene Expression Regulation, Developmental (日) (読) [継承]
11. (英) Genes (日) (読) [継承]
12. (英) Gestational Age (日) (読) [継承]
13. (英) Membranes (日) (読) [継承]
14. (英) Microarray Analysis (日) (読) [継承]
15. (英) Nucleic Acid Amplification Techniques (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Reproductive Biology and Endocrinology : RB&E ([BioMed Central Ltd.])
(eISSN: 1477-7827)

ISSN (任意): 1477-7827
ISSN: 1477-7827 (eISSN: 1477-7827)
Title: Reproductive biology and endocrinology : RB&E
Title(ISO): Reprod Biol Endocrinol
Publisher: BioMed Central
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 77 77 [継承]
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年月日 (必須): 西暦 2004年 11月 24日 (平成 16年 11月 24日) [継承]
URL (任意):
DOI (任意): 10.1186/1477-7827-2-77    (→Scopusで検索) [継承]
PMID (任意): 15560851    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Reproductive Biology and Technology Laboratory, Developmental Biology Department, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki 305-8602, Japan. ushizawa@affrc.go.jp <ushizawa@affrc.go.jp>  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Comparative Study  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC535809  (日)    [継承]

標準的な表示

和文冊子 ● Koichi Ushizawa, Chandana B. Herath, Kanako Kaneyama, Satoshi Shiojima, Akira Hirasawa, Toru Takahashi, Kei Imai, Kazuhiko Ochiai, Tomoyuki Tokunaga, Yukio Tsunoda, Gozoh Tsujimoto and Kazuyoshi Hashizume : cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period., Reproductive Biology and Endocrinology : RB&E, Vol.2, (号), 77, 2004.
欧文冊子 ● Koichi Ushizawa, Chandana B. Herath, Kanako Kaneyama, Satoshi Shiojima, Akira Hirasawa, Toru Takahashi, Kei Imai, Kazuhiko Ochiai, Tomoyuki Tokunaga, Yukio Tsunoda, Gozoh Tsujimoto and Kazuyoshi Hashizume : cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period., Reproductive Biology and Endocrinology : RB&E, Vol.2, (号), 77, 2004.

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