『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Chen Jie (日) (読)
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2. (英) Watanabe Masami (日) (読)
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[継承]
3. (英) Huang Peng (日) (読)
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4. (英) Sakaguchi Masakiyo (日) (読)
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[継承]
5.落合 和彦
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6. (英) Nasu Yasutomo (日) (読)
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7. (英) Ouchida Mamoru (日) (読)
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8. (英) Huh Nam-Ho (日) (読)
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9. (英) Shimizu Kenji (日) (読)
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10. (英) Kashiwakura Yuji (日) (読)
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11. (英) Kaku Haruki (日) (読)
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12. (英) Kumon Hiromi (日) (読)
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題名 (必須): (英) REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3, a member of the Dkk gene family, is a tumor suppressor in a broad range of cancers. REIC/Dkk-3 transfected stable clones of mouse prostate cancer RM9 cells (RM9-REIC) and the empty vector-transfected control clone cells (RM9-EV) were established. Clones were used to evaluate the anti-cancer effects and a proteomics analysis of REIC/Dkk-3 continuous expression was performed. The RM9-REIC cells show a feeble appearance and the cell membrane shows irregular buds known as blebs. In vitro cell proliferation was significantly suppressed in RM9-REIC clones in comparison to the control. The apoptosis assay was done under standard culture conditions and RM9-REIC showed a higher incidence of apoptosis. The RM9-EV and RM9-REIC cells were orthotopically implanted into a C57BL/6 mouse prostate. After 2 weeks, the tumor growth was significantly inhibited in RM9-REIC cells in comparison to the control. Two-dimensional gel electrophoresis was used to examine the modification of protein expression by the gene transfection. The analysis with mass spectrometry disclosed that expression of peroxiredoxin-1, GST-P1, transgelin-2, MRP-L12, ARD, GRP78 and Sorcin were increased and eEF1A-1 and cyclophilin-40 protein were decreased in RM9-REIC cells. Therefore, REIC/Dkk-3 stable transfectants show a reduction of malignancy in mouse prostate cancer RM9 cells in vitro and in vivo. The result of the proteomics analysis might provide important clues to clarify the anti-cancer molecular mechanism of REIC/Dkk-3 gene transfer.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2.アポトーシス (apoptosis) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Cell Growth Processes (日) (読) [継承]
5. (英) Cell Line, Tumor (日) (読) [継承]
6. (英) Cell Membrane (日) (読) [継承]
7. (英) Cell Survival (日) (読) [継承]
8. (英) Intercellular Signaling Peptides and Proteins (日) (読) [継承]
9. (英) Male (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Mice, Inbred C57BL (日) (読) [継承]
12. (英) Neoplasm Transplantation (日) (読) [継承]
13. (英) Phenotype (日) (読) [継承]
14. (英) Prostatic Neoplasms (日) (読) [継承]
15.プロテオミクス (proteomics) [継承]
16. (英) Transfection (日) (読) [継承]
17. (英) Tumor Burden (日) (読) [継承]
発行所 (推奨):
誌名 (必須): International Journal of Molecular Medicine (D.A. Spandidos)
(pISSN: 1107-3756, eISSN: 1791-244X)

ISSN (任意): 1791-244X
ISSN: 1107-3756 (pISSN: 1107-3756, eISSN: 1791-244X)
Title: International journal of molecular medicine
Title(ISO): Int J Mol Med
Publisher: Spandidos Publications
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 789 794 [継承]
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年月日 (必須): 西暦 2009年 12月 初日 (平成 21年 12月 初日) [継承]
URL (任意):
DOI (任意): 10.3892/ijmm_00000293    (→Scopusで検索) [継承]
PMID (任意): 19885619    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Jie Chen, Masami Watanabe, Peng Huang, Masakiyo Sakaguchi, Kazuhiko Ochiai, Yasutomo Nasu, Mamoru Ouchida, Nam-Ho Huh, Kenji Shimizu, Yuji Kashiwakura, Haruki Kaku and Hiromi Kumon : REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells., International Journal of Molecular Medicine, Vol.24, No.6, 789-794, 2009.
欧文冊子 ● Jie Chen, Masami Watanabe, Peng Huang, Masakiyo Sakaguchi, Kazuhiko Ochiai, Yasutomo Nasu, Mamoru Ouchida, Nam-Ho Huh, Kenji Shimizu, Yuji Kashiwakura, Haruki Kaku and Hiromi Kumon : REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells., International Journal of Molecular Medicine, Vol.24, No.6, 789-794, 2009.

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