『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kashiwakura Yuji (日) (読)
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2.落合 和彦
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3. (英) Watanabe Masami (日) (読)
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4. (英) Abarzua Fernando (日) (読)
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5. (英) Sakaguchi Masakiyo (日) (読)
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6. (英) Takaoka Munenori (日) (読)
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7. (英) Tanimoto Ryuta (日) (読)
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8. (英) Nasu Yasutomo (日) (読)
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9. (英) Huh Nam-Ho (日) (読)
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10. (英) Kumon Hiromi (日) (読)
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題名 (必須): (英) Down-regulation of inhibition of differentiation-1 via activation of activating transcription factor 3 and Smad regulates REIC/Dickkopf-3-induced apoptosis.  (日)    [継承]
副題 (任意):
要約 (任意): (英) REIC/Dickkopf-3 (Dkk-3), a tumor suppressor gene, has been investigated in gene therapy studies. Our previous study suggested that REIC/Dkk-3-induced apoptosis mainly resulted from phosphorylation of c-Jun-NH(2) kinase (JNK) in prostate cancer cells. However, the precise mechanisms, especially the molecular mechanisms regulating JNK phosphorylation, remain unclear. In this study, we investigated the mechanisms participating in JNK phosphorylation in the context of a refractory cancer disease, malignant mesothelioma (MM). Adenovirus-mediated overexpression of REIC/Dkk-3 induced apoptosis mainly through JNK activation in immortalized MM cells (211H cells). Interestingly, transcriptional down-regulation of inhibition of differentiation-1 (Id-1) was detected in REIC/Dkk-3-overexpressed 211H cells. Moreover, restoration of Id-1 expression antagonized REIC/Dkk-3-induced JNK phosphorylation and apoptosis. Mutagenesis experiments with the 2.1-kb human Id-1 promoter revealed that activating transcription factor 3 (ATF3) and Smad interaction, with their respective binding motifs, was essential for REIC/Dkk-3-mediated suppression of Id-1 promoter activity. ATF3 activation was probably induced by endoplasmic reticulum stress. Finally, we showed strong antitumor effects from REIC/Dkk-3 gene transfer into the pleural cavity in an orthotopic MM mouse model. Relative to control tumor tissue, REIC/Dkk-3-treated tumor tissue showed down-regulated expression of Id-1 mRNA, enhanced expression of phosphorylated JNK, and an increased number of apoptotic cells. In summary, we first showed that both ATF3 and Smad were crucially and synergistically involved in down-regulation of Id-1, which regulated JNK phosphorylation in REIC/Dkk-3-induced apoptosis. Thus, gene therapy with REIC/Dkk-3 may be a promising therapeutic tool for MM.  (日)    [継承]
キーワード (推奨): 1. (英) Activating Transcription Factor 3 (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3.アポトーシス (apoptosis) [継承]
4. (英) Down-Regulation (日) (読) [継承]
5.小胞体 (endoplasmic reticulum) [継承]
6.遺伝子治療 (gene therapy) [継承]
7. (英) Hela Cells (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Inhibitor of Differentiation Protein 1 (日) (読) [継承]
10. (英) Intercellular Signaling Peptides and Proteins (日) (読) [継承]
11. (英) JNK Mitogen-Activated Protein Kinases (日) (読) [継承]
12. (英) Mice (日) (読) [継承]
13. (英) Mice, Inbred BALB C (日) (読) [継承]
14. (英) Multiple Myeloma (日) (読) [継承]
15. (英) NF-kappa B (日) (読) [継承]
16.リン酸化 (phosphorylation) [継承]
17. (英) Promoter Regions, Genetic (日) (読) [継承]
18. (英) Smad Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Cancer Research (American Assotiation for Cancer Research)
(pISSN: 0008-5472, eISSN: 1538-7445)

ISSN (任意): 1538-7445
ISSN: 0008-5472 (pISSN: 0008-5472, eISSN: 1538-7445)
Title: Cancer research
Title(ISO): Cancer Res
Publisher: American Association for Cancer Research
 (NLM Catalog  (Scopus  (Scopus  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 8333 8341 [継承]
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年月日 (必須): 西暦 2008年 10月 15日 (平成 20年 10月 15日) [継承]
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DOI (任意): 10.1158/0008-5472.CAN-08-0080    (→Scopusで検索) [継承]
PMID (任意): 18922905    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Innovation Center Okayama for Nanobio-Targeted Therapy, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan. yu-kashi@cj9.so-net.ne.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yuji Kashiwakura, Kazuhiko Ochiai, Masami Watanabe, Fernando Abarzua, Masakiyo Sakaguchi, Munenori Takaoka, Ryuta Tanimoto, Yasutomo Nasu, Nam-Ho Huh and Hiromi Kumon : Down-regulation of inhibition of differentiation-1 via activation of activating transcription factor 3 and Smad regulates REIC/Dickkopf-3-induced apoptosis., Cancer Research, Vol.68, No.20, 8333-8341, 2008.
欧文冊子 ● Yuji Kashiwakura, Kazuhiko Ochiai, Masami Watanabe, Fernando Abarzua, Masakiyo Sakaguchi, Munenori Takaoka, Ryuta Tanimoto, Yasutomo Nasu, Nam-Ho Huh and Hiromi Kumon : Down-regulation of inhibition of differentiation-1 via activation of activating transcription factor 3 and Smad regulates REIC/Dickkopf-3-induced apoptosis., Cancer Research, Vol.68, No.20, 8333-8341, 2008.

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