| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | 廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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| 2. | 板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 3. | 片岡 正俊
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| 4. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 5. | (英) Herzberg MC (日) (読)
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| 6. | (英) Ross KF (日) (読)
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| 7. | 稲垣 裕司 ([徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 8. | 永田 俊彦
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| 9. | 木戸 淳一
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| ○題名 (必須): | □ | (英) Shosaikoto increases calprotectin expression in human oral epithelial cells. (日)
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| ○要約 (任意): | □ | (英) BACKGROUND AND OBJECTIVE: Oral epithelial cells help to prevent against bacterial infection in the oral cavity by producing antimicrobial peptides (AMPs). A broad-spectrum AMP, calprotectin (a complex of S100A8 and S100A9 proteins), is expressed by oral epithelial cells and is up-regulated by interleukin-1alpha (IL-1alpha). Shosaikoto (SST) is a traditional Japanese herbal medicine that has immunomodulatory effects and is reported to enhance the levels of IL-1alpha in epithelial cells. The purpose of this study was to investigate the effect of SST on the expression of calprotectin and other AMPs through the regulation of IL-1alpha in oral epithelial cells. MATERIAL AND METHODS: Human oral epithelial cells (TR146) were cultured with SST (at concentrations ranging from 10 to 250 microg/mL) in the presence or absence of anti-IL-1alpha or IL-1 receptor antagonist. The expression of S100A8- and S100A9-specific mRNAs was examined by northern blotting. Calprotectin expression and IL-1alpha secretion were investigated by immunofluorescent staining or ELISA. The expression of other AMPs and IL-1alpha was analyzed by RT-PCR and by quantitative real-time PCR. RESULTS: Shosaikoto (25 microg/mL) significantly increased the expression of S100A8- and S100A9-specific mRNAs and calprotectin protein. Shosaikoto increased S100A7 expression, but had no effect on the expression of other AMPs. The expression of IL-1alpha-specific mRNA and its protein were slightly increased by SST. A neutralizing antibody against IL-1alpha or IL-1 receptor antagonist inhibited SST up-regulated S100A8/S100A9 mRNA expression. CONCLUSION: These results suggest that SST increases the expression of calprotectin and S100A7 in oral epithelial cells. In response to SST, up-regulation of calprotectin may be partially induced via IL-1alpha. (日)
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| ○誌名 (必須): | □ | Journal of Periodontal Research ([Blackwell Publishing Ltd])
(pISSN: 0022-3484, eISSN: 1600-0765)
| ○ISSN (任意): | □ | 1600-0765
ISSN: 0022-3484
(pISSN: 0022-3484, eISSN: 1600-0765) Title: Journal of periodontal researchTitle(ISO): J Periodontal ResPublisher: Blackwell (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 45
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 79 86
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| ○年月日 (必須): | □ | 西暦 2010年 7月 8日 (平成 22年 7月 8日)
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| ○DOI (任意): | □ | 10.1111/j.1600-0765.2009.01203.x (→Scopusで検索)
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| ○PMID (任意): | □ | 19602113 (→Scopusで検索)
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| ○WOS (任意): | □ | 000273667900011
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| ○備考 (任意): | 1. | (英) Affiliation: Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. (日)
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| 2. | (英) PublicationType: Journal Article (日)
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| 3. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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