『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.発達予防医歯学部門.病態予防医学講座.生理機能学 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.医学部.医学科.病態予防医学講座.細胞生物学分野 [継承]
著者 (必須): 1.稲垣 明浩 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.生理学])
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2. (英) Yamaguchi Soichiro (日) (読)
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3. (英) Takahashi-Iwanaga Hiromi (日) (読)
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4. (英) Iwanaga Toshihiko (日) (読)
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5. (英) Ishikawa Toru (日) (読)
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題名 (必須): (英) Functional characterization of a ClC-2-like Cl(-) conductance in surface epithelial cells of rat rectal colon.  (日)    [継承]
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要約 (任意): (英) ClC-2, a member of the voltage-gated Cl(-) channel family, is expressed in the distal colonic surface epithelial cells of various species, but its functional significance remains unclear. Here, by means of electrophysiological and molecular biological techniques, we have identified and characterized a ClC-2-like conductance naturally expressed by surface epithelial cells acutely dissociated from rectal colon of rats fed a standard diet. Whole-cell patch-clamp experiments showed that the surface cells, whether an amiloride-sensitive Na(+) conductance was present or not, displayed a strong hyperpolarization-activated, inwardly rectifying Cl(-) current. Analysis both by in situ hybridization and immunohistochemistry confirmed the expression of ClC-2 in the rectal surface epithelium. The native Cl(-) current shared common electrophysiological properties including voltage-dependent activation, anion selectivity sequence, and Zn(2+) sensitivity with that recorded from HEK293 cells transfected with ClC-2 cloned from rat rectal colon (rClC-2). Cell-attached patch recordings on the surface cells revealed that native ClC-2-like currents activated only at potentials at least 40 mV more negative than resting membrane potentials. In Ussing chamber experiments with rat rectal mucosa, either basolateral or apical application of Zn(2+) (0.1 mM), which inhibited both native ClC-2-like currents and recombinant rClC-2 currents, had little, if any, effects on basal amiloride-sensitive short-circuit current. Collectively, these results not only demonstrate that a functional ClC-2-type Cl(-) channel is expressed in rat rectal surface epithelium, but also suggest that the channel activity may be negligible and thus nonessential for controlling electrogenic Na(+) transport in this surface epithelium under basal physiological conditions.  (日)    [継承]
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誌名 (必須): The Journal of Membrane Biology ([Springer-Verlag])
(pISSN: 0022-2631, eISSN: 1432-1424)

ISSN (任意): 1432-1424
ISSN: 0022-2631 (pISSN: 0022-2631, eISSN: 1432-1424)
Title: The Journal of membrane biology
Title(ISO): J Membr Biol
Supplier: Springer Online Journal Archive
Publisher: Springer
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(必須): 235 [継承]
(必須): 1 [継承]
(必須): 27 41 [継承]
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年月日 (必須): 西暦 2010年 4月 22日 (平成 22年 4月 22日) [継承]
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DOI (任意): 10.1007/s00232-010-9253-6    (→Scopusで検索) [継承]
PMID (任意): 20411246    (→Scopusで検索) [継承]
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WOS (任意): 000277341400003 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Akihiro Inagaki, Soichiro Yamaguchi, Hiromi Takahashi-Iwanaga, Toshihiko Iwanaga and Toru Ishikawa : Functional characterization of a ClC-2-like Cl(-) conductance in surface epithelial cells of rat rectal colon., The Journal of Membrane Biology, Vol.235, No.1, 27-41, 2010.
欧文冊子 ● Akihiro Inagaki, Soichiro Yamaguchi, Hiromi Takahashi-Iwanaga, Toshihiko Iwanaga and Toru Ishikawa : Functional characterization of a ClC-2-like Cl(-) conductance in surface epithelial cells of rat rectal colon., The Journal of Membrane Biology, Vol.235, No.1, 27-41, 2010.

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