『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=207377EID:207377, Map:0, LastModified:2013年8月3日(土) 19:21:33, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[吉丸 哲郎], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Suzuki Yoshihiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.吉丸 哲郎 ([徳島大学.先端酵素学研究所.重点研究部門])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Matsui Takashi (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Inoue Toshio (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Niide Osamu (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Nunomura Satoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Ra Chisei (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Fc epsilon RI signaling of mast cells activates intracellular production of hydrogen peroxide: role in the regulation of calcium signals.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Earlier studies, including our own, revealed that activation of mast cells is accompanied by production of reactive oxygen species (ROS) that help to mediate the release of the inflammatory mediators, including histamine and eicosanoids. However, little is known about the mechanisms of ROS production, including the species of oxidants produced. In this study we show that in both the RBL-2H3 mast cell line and bone marrow-derived mast cells, FcepsilonRI cross-linking stimulates intracellular oxidative burst, including hydrogen peroxide (H(2)O(2)) production, as defined with the oxidant-sensitive dyes dichlorofluorescein and scopoletin and the selective scavenger ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one). The oxidative burst was observed immediately after stimulation and was most likely due to an NAD(P)H oxidase. Experiments using selective pharmacological inhibitors demonstrated that activation of tyrosine kinases and phosphatidylinositol-3-kinase is required for induction of the oxidative burst. Blockade of the oxidative burst by diphenyleneiodonium impaired the release of preformed granular mediators, such as histamine and beta-hexosaminidase, and the secretion of newly synthesized leukotriene C(4), whereas selective scavenging H(2)O(2) by ebselen impaired leukotriene C(4) secretion, but not degranulation. Sustained elevation of cytosolic calcium through store-operated calcium entry was totally abolished when ROS production was blocked. In contrast, selective depletion of H(2)O(2) caused a considerable decrease and delay of the calcium response. Finally, tyrosine phosphorylation of phospholipase Cgamma and the linker for activation of T cells, an event required for calcium influx, was suppressed by diphenyleneiodonium and ebselen. These studies demonstrate that activation of the intracellular oxidative burst is an important regulatory mechanism of mast cell responses.  (日)    [継承]
キーワード (推奨): 1. (英) 1-Phosphatidylinositol 3-Kinase (日) (読) [継承]
2. (英) Adaptor Proteins, Signal Transducing (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Antioxidants (日) (読) [継承]
5. (英) Calcium (日) (読) [継承]
6. (英) Calcium Signaling (日) (読) [継承]
7. (英) Carrier Proteins (日) (読) [継承]
8. (英) Cell Line, Tumor (日) (読) [継承]
9. (英) Hydrogen Peroxide (日) (読) [継承]
10. (英) Intracellular Fluid (日) (読) [継承]
11. (英) Mast Cells (日) (読) [継承]
12. (英) Membrane Proteins (日) (読) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) NADH, NADPH Oxidoreductases (日) (読) [継承]
15. (英) NADPH Oxidase (日) (読) [継承]
16. (英) Onium Compounds (日) (読) [継承]
17. (英) Phospholipase C gamma (日) (読) [継承]
18. (英) Phosphoproteins (日) (読) [継承]
19. (英) Phosphorylation (日) (読) [継承]
20. (英) Phosphotyrosine (日) (読) [継承]
21. (英) Protein-Tyrosine Kinases (日) (読) [継承]
22. (英) Rats (日) (読) [継承]
23. (英) Reactive Oxygen Species (日) (読) [継承]
24. (英) Receptors, IgE (日) (読) [継承]
25. (英) Respiratory Burst (日) (読) [継承]
26. (英) T-Lymphocytes (日) (読) [継承]
27. (英) Type C Phospholipases (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN (任意): 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 171 [継承]
(必須): 11 [継承]
(必須): 6119 6127 [継承]
都市 (任意):
年月日 (必須): 西暦 2003年 12月 1日 (平成 15年 12月 1日) [継承]
URL (任意):
DOI (任意):
PMID (任意): 14634127    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000186767200060 [継承]
Scopus (任意):
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備考 (任意): 1.(英) Affiliation: Division of Molecular Cell Immunology and Allergology, Nihon University Graduate School of Medical Sciences, Tokyo, Japan. ysuzuki@med.nihon-u.ac.jp  (日)    [継承]
2.(英) PublicationType: Journal Article  (日)    [継承]
3.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yoshihiro Suzuki, Tetsuro Yoshimaru, Takashi Matsui, Toshio Inoue, Osamu Niide, Satoshi Nunomura and Chisei Ra : Fc epsilon RI signaling of mast cells activates intracellular production of hydrogen peroxide: role in the regulation of calcium signals., The Journal of Immunology, Vol.171, No.11, 6119-6127, 2003.
欧文冊子 ● Yoshihiro Suzuki, Tetsuro Yoshimaru, Takashi Matsui, Toshio Inoue, Osamu Niide, Satoshi Nunomura and Chisei Ra : Fc epsilon RI signaling of mast cells activates intracellular production of hydrogen peroxide: role in the regulation of calcium signals., The Journal of Immunology, Vol.171, No.11, 6119-6127, 2003.

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