『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hidaka Kyoko (日) (読)
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2.新田 剛
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[継承]
3. (英) Sugawa Ryo (日) (読)
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[継承]
4. (英) Schwartz Robert J (日) (読)
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5. (英) Amagai Takashi (日) (読)
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学籍番号 (推奨):
[継承]
6. (英) Nitta Sachiko (日) (読)
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7.高浜 洋介
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8. (英) Morisaki Takayuki (日) (読)
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題名 (必須): (英) Differentiation of Pharyngeal Endoderm and Derivatives from Mouse Embryonic Stem Cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Embryonic stem cells are considered to be a good in vitro tool to study the induction of various cell types including cardiomyocytes; however, induction of the pharyngeal endoderm (PE), the underlying heart-forming region, in vivo has been scarcely reported. In the present study, we found that many PE-related genes, such as Paxl, Pax9, Sixl, and Tbxl, were up-regulated in cardiomyocyte-rich embryoid bodies (EBs). The third pouch-related genes including Hoxa3, Foxn1, and Aire, which are crucial for thymus development and function, were also detected in later stages. Nkx2.5, a cardiac transcription factor gene, is known to be transiently expressed in the PE. By crossing Nkx2.5-Cre mice with Cre-dependent EGFP reporter mice, we found that Nkx2.5(+) lineage exclusively contributed to thymic epithelial cell development, followed by thymus development. Gene expression analysis using Nkx2.5-EGFP ES cells also revealed that PE-related mRNAs were specifically enriched in the transiently appearing E-cadherin(+)Nkx2.5(+) cell fraction. Interestingly, the EB-derived cells were found capable of supporting T-cell differentiation to CD4 or CD8 double-positive cells in a reaggregation organ culture in vitro. Our results suggest that EBs contain cells that resemble third pharyngeal pouch endoderm and confer a thymus-like microenvironment.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cell Differentiation (日) (読) [継承]
3. (英) Cell Lineage (日) (読) [継承]
4. (英) Cells, Cultured (日) (読) [継承]
5. (英) Coculture Techniques (日) (読) [継承]
6. (英) Embryoid Bodies (日) (読) [継承]
7. (英) Embryonic Stem Cells (日) (読) [継承]
8. (英) Endoderm (日) (読) [継承]
9. (英) Homeodomain Proteins (日) (読) [継承]
10. (英) Kidney (日) (読) [継承]
11. (英) Mice (日) (読) [継承]
12. (英) Mice, Transgenic (日) (読) [継承]
13. (英) Pharynx (日) (読) [継承]
14. (英) Thymus Gland (日) (読) [継承]
15. (英) Transcription Factors (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Stem Cells and Development (Mary Ann Liebert, Inc.)
(pISSN: 1547-3287, eISSN: 1557-8534)

ISSN (任意): 1557-8534
ISSN: 1547-3287 (pISSN: 1547-3287, eISSN: 1557-8534)
Title: Stem cells and development
Title(ISO): Stem Cells Dev
Publisher: Mary Ann Liebert Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 19 [継承]
(必須): 11 [継承]
(必須): 1735 1743 [継承]
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年月日 (必須): 西暦 2010年 11月 末日 (平成 22年 11月 末日) [継承]
URL (任意):
DOI (任意): 10.1089/scd.2009.0466    (→Scopusで検索) [継承]
PMID (任意): 20230268    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000283544200009 [継承]
Scopus (任意): 2-s2.0-78049383117 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Bioscience, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kyoko Hidaka, Takeshi Nitta, Ryo Sugawa, J Robert Schwartz, Takashi Amagai, Sachiko Nitta, Yousuke Takahama and Takayuki Morisaki : Differentiation of Pharyngeal Endoderm and Derivatives from Mouse Embryonic Stem Cells., Stem Cells and Development, Vol.19, No.11, 1735-1743, 2010.
欧文冊子 ● Kyoko Hidaka, Takeshi Nitta, Ryo Sugawa, J Robert Schwartz, Takashi Amagai, Sachiko Nitta, Yousuke Takahama and Takayuki Morisaki : Differentiation of Pharyngeal Endoderm and Derivatives from Mouse Embryonic Stem Cells., Stem Cells and Development, Vol.19, No.11, 1735-1743, 2010.

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