『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1.佐藤 陽一 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品情報学])
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2.新家 利一
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3. (英) Sakamoto Kozue (日) (読)
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4.エヴィス アシュラフ
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5.中堀 豊
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題名 (必須): (英) The male-determining gene SRY is a hybrid of DGCR8 and SOX3, and is regulated by the transcription factor CP2.  (日)    [継承]
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要約 (任意): (英) In mammals, sex is determined by the presence or absence of the Y chromosome that bears a male-dominant sex-determining gene SRY, which switches the differentiation of gonads into male testes. The molecular signaling mechanism turning on the switch, however, has remained unclear for 18 years since the identification of the gene. Here, we describe how this gene emerged and started to work. From amino acid homology, we realized that SRY is a hybrid gene between a portion of the first exon of DiGeorge syndrome critical region gene 8 (DGCR8) and the high-mobility group (HMG) box of SRY box-3 (SOX3) gene. We identified the regulatory sequence in the SRY promotor region by searching for a common motif shared with DGCR8 mRNA. From the motif search between DGCR8 mRNA and the SRY upstream sequence, we found that the transcription factor CP2 (TFCP2) binding motif is present in both. TFCP2 overexpression did not show a significant increase of SRY mRNA expression, and TFCP2 suppression by RNA interference (RNAi) significantly reduced SRY mRNA expression. Furthermore, electrophoretic mobility shift assay (EMSA) demonstrated that TFCP2 acts as a regulator by directly binding to the SRY promoter. We conclude that SRY is a hybrid gene composed of two genes, DGCR8 and SOX3; and TFCP2 is an essential transcription factor for SRY expression regulation.  (日)    [継承]
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誌名 (必須): Molecular and Cellular Biochemistry (Springer)
(pISSN: 0300-8177, eISSN: 1573-4919)

ISSN (任意): 1573-4919
ISSN: 0300-8177 (pISSN: 0300-8177, eISSN: 1573-4919)
Title: Molecular and cellular biochemistry
Title(ISO): Mol Cell Biochem
Supplier: Kluwer Online
Publisher: Springer US
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年月日 (必須): 西暦 2010年 3月 初日 (平成 22年 3月 初日) [継承]
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DOI (任意): 10.1007/s11010-009-0308-x    (→Scopusで検索) [継承]
PMID (任意): 19902333    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan, sato@basic.med.tokushima-u.ac.jp.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: JOURNAL ARTICLE  (日)    [継承]

標準的な表示

和文冊子 ● Youichi Sato, Toshikatsu Shinka, Kozue Sakamoto, ASHRAF ABDEL AZIM EWIS and Yutaka Nakahori : The male-determining gene SRY is a hybrid of DGCR8 and SOX3, and is regulated by the transcription factor CP2., Molecular and Cellular Biochemistry, Vol.337, No.1-2, 267-275, 2010.
欧文冊子 ● Youichi Sato, Toshikatsu Shinka, Kozue Sakamoto, ASHRAF ABDEL AZIM EWIS and Yutaka Nakahori : The male-determining gene SRY is a hybrid of DGCR8 and SOX3, and is regulated by the transcription factor CP2., Molecular and Cellular Biochemistry, Vol.337, No.1-2, 267-275, 2010.

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