| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Matsunaga Takuya (日) (読)
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| 2. | (英) Tanaka Ikuta (日) (読)
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| 3. | (英) Kobune Masayoshi (日) (読)
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| 4. | (英) Kawano Yutaka (日) (読)
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| 5. | (英) Tanaka Maki (日) (読)
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| 6. | (英) Kuribayashi Kageaki (日) (読)
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| 7. | (英) Iyama Satoshi (日) (読)
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| 8. | (英) Sato Tsutomu (日) (読)
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| 9. | 佐藤 康史 ([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).地域消化器・総合内科学])
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| 10. | (英) Takimoto Rishu (日) (読)
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| 11. | 高山 哲治 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学])
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| 12. | (英) Kato Junji (日) (読)
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| 13. | (英) Ninomiya Takafumi (日) (読)
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| 14. | (英) Hamada Hirofumi (日) (読)
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| 15. | (英) Niitsu Yoshiro (日) (読)
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| ○題名 (必須): | □ | (英) Ex vivo large scale generation of human platelets from cord blood CD34+ cells. (日)
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| ○要約 (任意): | □ | (英) In the present investigation, we generated platelets (PLTs) from cord blood (CB) CD34(+) cells using a three-phase culture system. We first cultured 500 CB CD34(+) cells on telomerase gene-transduced human stromal cells (hTERT stroma) in serum-free medium supplemented with stem cell factor (SCF), Flt-3/Flk-2 ligand (FL), and thrombopoietin (TPO) for 14 days. We then transferred the cells to hTERT stroma and cultured for another 14 days with fresh medium containing interleukin-11 (IL-11) in addition to the original cytokine cocktail. Subsequently, we cultured the cells in a liquid culture medium containing SCF, FL, TPO, and IL-11 for another 5 days to recover PLT fractions from the supernatant, which were then gel-filtered to purify the PLTs. The calculated yield of PLTs from 1.0 unit of CB (5 x 10(6) CD34(+) cells) was 1.26 x 10(11) - 1.68 x 10(11) PLTs. These numbers of PLTs are equivalent to 2.5-3.4 units of random donor-derived PLTs or 2/5-6/10 of single-apheresis PLTs. The CB-derived PLTs exhibited features quite similar to those from peripheral blood in morphology, as revealed by electron micrographs, and in function, as revealed by fibrinogen/ADP aggregation, with the appearance of P-selectin and activated glycoprotein IIb-IIIa antigens. Thus, this culture system may be applicable for large-scale generation of PLTs for future clinical use. (日)
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| ○キーワード (推奨): | 1. | (英) Antigens, CD34 (日) (読)
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| 2. | (英) Blood Platelets (日) (読)
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| 3. | (英) Cell Culture Techniques (日) (読)
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| 4. | 細胞分化 (cell differentiation)
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| 5. | (英) Cell Lineage (日) (読)
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| 6. | 細胞質分裂 (cytokinesis)
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| 7. | (英) Endothelial Cells (日) (読)
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| 8. | (英) Fetal Blood (日) (読)
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| 9. | (英) HLA Antigens (日) (読)
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| 10. | (英) Humans (日) (読)
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| 11. | (英) Megakaryocytes (日) (読)
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| 12. | (英) Platelet Membrane Glycoprotein IIb (日) (読)
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| 13. | (英) Ploidies (日) (読)
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| 14. | (英) Stem Cells (日) (読)
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| 15. | (英) Stromal Cells (日) (読)
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| 16. | (英) Telomerase (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Stem Cells (AlphaMed Press)
(pISSN: 1066-5099, eISSN: 1549-4918)
| ○ISSN (任意): | □ | 1066-5099
ISSN: 1066-5099
(pISSN: 1066-5099, eISSN: 1549-4918) Title: Stem cells (Dayton, Ohio)Title(ISO): Stem CellsSupplier: AlphaMed PressPublisher: Oxford University Press (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 24
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| ○号 (必須): | □ | 12
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| ○頁 (必須): | □ | 2877 2887
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2006年 9月 7日 (平成 18年 9月 7日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1634/stemcells.2006-0309 (→Scopusで検索)
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| ○PMID (任意): | □ | 16960134 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Fourth Department of Internal Medicine, Sapporo Medical University, School of Medicine, S1W17, Sapporo, Japan. (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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