『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=194131EID:194131, Map:0, LastModified:2012年10月3日(水) 12:00:17, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[辻 明彦], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ソシオテクノサイエンス研究部.ライフシステム部門.生命情報工学 (2006年4月1日〜2016年3月31日) [継承]
著者 (必須): 1.湯浅 恵造 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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学籍番号 (推奨):
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2. (英) Masuda Tetsuya (日) (読)
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[継承]
3. (英) Yoshikawa Chihiro (日) (読)
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[継承]
4.長浜 正巳
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5.松田 佳子
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6.辻 明彦
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Subtilisin-like Proprotein Convertase PACE4 is Required for Skeletal Muscle Differentiation  (日)    [継承]
副題 (任意):
要約 (任意): (英) Most growth factors stimulate myoblast proliferation and prevent differentiation, whereas insulin-like growth factors (IGFs) promote myoblast differentiation through the phosphatidylinositol 3-kinase (PI3K) pathway. Subtilisin-like proprotein convertases (SPCs) are involved in cell growth and differentiation via activation of pro-growth factors. However, the role of SPCs in myogenesis remains poorly understood. Here we show that PACE4, a member of the SPC family, plays a critical role in myogenic differentiation of C2C12 cells. PACE4 mRNA levels increased markedly during myogenesis, whereas the expression of other member of SPC family, furin and PC6, remained unchanged. The expression pattern of pro-IGF-II, which is processed extracellularly by SPCs, was similar to that of PACE4. The expression of shRNA targeting PACE4, but not furin, suppressed the expression of the muscle-specific myosin light chain (MLC). Interestingly, reduced expression of MLC was restored following treatment with recombinant mature IGF-II. Finally, we demonstrated that the PI3K inhibitor LY294002 blocked the induction of PACE4 mRNA, a result not observed when another myogenic differentiation inhibitor, SB203580 (p38 MAP kinase inhibitor), was employed, indicating the presence of a positive feedback loop regulating PACE4 expression. These results suggest that PACE4 plays an important role in myogenic differentiation through its association with the IGF-II pathway.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cell Differentiation (日) (読) [継承]
3. (英) Cell Line (日) (読) [継承]
4. (英) Feedback, Physiological (日) (読) [継承]
5. (英) Gene Knockdown Techniques (日) (読) [継承]
6. (英) Insulin-Like Growth Factor II (日) (読) [継承]
7. (英) Matrix Metalloproteinase 11 (日) (読) [継承]
8. (英) Mice (日) (読) [継承]
9. (英) Muscle Development (日) (読) [継承]
10. (英) MyoD Protein (日) (読) [継承]
11. (英) Myoblasts, Skeletal (日) (読) [継承]
12. (英) Myogenin (日) (読) [継承]
13. (英) Myosin Light Chains (日) (読) [継承]
14. (英) Phosphatidylinositol 3-Kinases (日) (読) [継承]
15. (英) Proprotein Convertases (日) (読) [継承]
16. (英) Protein Precursors (日) (読) [継承]
17. (英) Signal Transduction (日) (読) [継承]
18. (英) Up-Regulation (日) (読) [継承]
19. (英) p38 Mitogen-Activated Protein Kinases (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 1756-2651
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J Biochem
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 407 415 [継承]
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年月日 (必須): 西暦 2009年 6月 11日 (平成 21年 6月 11日) [継承]
URL (任意):
DOI (任意): 10.1093/jb/mvp090    (→Scopusで検索) [継承]
PMID (任意): 19520771    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biological Science, The University of Tokushima Graduate School, 2-1 Minamijosanjima, Tokushima 770-8506, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Keizo Yuasa, Tetsuya Masuda, Chihiro Yoshikawa, Masami Nagahama, Yoshiko Matsuda and Akihiko Tsuji : Subtilisin-like Proprotein Convertase PACE4 is Required for Skeletal Muscle Differentiation, The Journal of Biochemistry, Vol.146, No.3, 407-415, 2009.
欧文冊子 ● Keizo Yuasa, Tetsuya Masuda, Chihiro Yoshikawa, Masami Nagahama, Yoshiko Matsuda and Akihiko Tsuji : Subtilisin-like Proprotein Convertase PACE4 is Required for Skeletal Muscle Differentiation, The Journal of Biochemistry, Vol.146, No.3, 407-415, 2009.

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