『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
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学究種別 (推奨):
組織 (推奨): 1.徳島大学.工学部.生物工学科.生物機能工学講座 [継承]
著者 (必須): 1. (英) Nishimoto Makoto (日) (読)
役割 (任意): (英)   (日) 記述,考察   [継承]
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2. (英) Morimitsu Tohru (日) (読)
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3.玉井 伸岳 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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4.金品 昌志
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5.長宗 秀明 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
役割 (任意): (英)   (日) 考察   [継承]
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6.松木 均 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
役割 (任意): (英)   (日) 計画,解析,考察,論文執筆の指導   [継承]
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題名 (必須): (英) Inhibition of anti-fluorescent probe monoclonal antibody by long-chain amphiphiles  (日)    [継承]
副題 (任意):
要約 (任意): (英) Inhibitions of amphiphiles with a long chain (alcohols with carbon number of 10, 12, 14, 16 and the corresponding fatty acids) on the antigen-antibody reaction between fluorescent probe (fluorescein (FL)) and anti-fluorescent probe monoclonal antibody (immunoglobulin G (IgG)-49) were investigated by fluorescence spectroscopy. The resulting inhibition modes were compared with those of specific inhibitors, 5- and 6-aminofluoresceins (5- and 6-FLNH(2)). In the presence of all inhibitors, the fluorescence quenching of FL as the result of inhibitor binding to IgG-49 was hindered in an inhibitor concentration-dependent manner. The values of IC(50) and Hill number for the inhibitors were determined from dose-response curves of the quenching inhibition. The IC(50) values of the alcohols were much more dependent on the chain length than those of the fatty acids although all the values were in orders of microM. The Hill numbers of both kinds of inhibitors became larger values than 1, but they had different values for a series of alcohols and fatty acids, respectively. By contrast, the IC(50) values of 5- and 6-FLNH(2) were in orders of nM and the Hill numbers became smaller values than 1. Since the Lineweaver-Burk plots produced an intersection point on the intercept of the X-axis for the long-chain inhibitors with carbon number of 14 while did that on the intercept of the Y-axis for 6-FLNH(2), respectively, we judged the inhibition modes of the former as non-competitive ones and those of the latter as a competitive one. Further, the effect of pressure on the inhibition by C(14)OH, C(13)COOH and 5-FLNH(2) was examined, and the standard volume changes of IgG-49 for the inhibitor binding were calculated from the Johnson-Eyring plots. The volume change for 5-FLNH(2) was smaller than those for C(14)OH and C(13)COOH although they became all negative. This suggests that the volume of IgG-49 shrinks by the addition of these inhibitors, and IgG-49 is not relevant to a model protein for the molecular mechanism of anesthesia.  (日)    [継承]
キーワード (推奨): 1. (英) Alcohols (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Antibodies, Monoclonal (日) (読) [継承]
4. (英) Fluorescein (日) (読) [継承]
5. (英) Fluorescent Dyes (日) (読) [継承]
6. (英) Inhibitory Concentration 50 (日) (読) [継承]
7. (英) Kinetics (日) (読) [継承]
8. (英) Ligands (日) (読) [継承]
9. (英) Mice (日) (読) [継承]
10. (英) Pressure (日) (読) [継承]
発行所 (推奨): Elsevier Science [継承]
誌名 (必須): Colloids and Surfaces B:Biointerfaces ([Elsevier])
(pISSN: 0927-7765, eISSN: 1873-4367)

ISSN (任意): 1873-4367
ISSN: 0927-7765 (pISSN: 0927-7765, eISSN: 1873-4367)
Title: Colloids and surfaces. B, Biointerfaces
Title(ISO): Colloids Surf B Biointerfaces
Publisher: Elsevier BV
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年月日 (必須): 西暦 2010年 1月 1日 (平成 22年 1月 1日) [継承]
URL (任意):
DOI (任意): 10.1016/j.colsurfb.2009.08.012    (→Scopusで検索) [継承]
PMID (任意): 19740635    (→Scopusで検索) [継承]
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WOS (任意): 000278695300010 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minamijosanjima-cho, Tokushima 770-8506, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Makoto Nishimoto, Tohru Morimitsu, Nobutake Tamai, Shoji Kaneshina, Hideaki Nagamune and Hitoshi Matsuki : Inhibition of anti-fluorescent probe monoclonal antibody by long-chain amphiphiles, Colloids and Surfaces B:Biointerfaces, Vol.75, No.1, 80-87, 2010.
欧文冊子 ● Makoto Nishimoto, Tohru Morimitsu, Nobutake Tamai, Shoji Kaneshina, Hideaki Nagamune and Hitoshi Matsuki : Inhibition of anti-fluorescent probe monoclonal antibody by long-chain amphiphiles, Colloids and Surfaces B:Biointerfaces, Vol.75, No.1, 80-87, 2010.

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