『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=191544)

EID=191544EID:191544, Map:0, LastModified:2018年4月20日(金) 09:23:50, Operator:[野間 隆文], Avail:TRUE, Censor:0, Owner:[三好 圭子], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Ruspita Intan (日) INTAN RUSPITA (読) いんたん るすぴた
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学籍番号 (推奨): **** [ユーザ]
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2.三好 圭子 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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3.武藤 太郎
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4.阿部 佳織
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5.堀口 大吾 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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6.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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題名 (必須): (英) Sp6 downregulation of follistatin gene expression in ameloblasts.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Sp6 is a member of the Sp family of transcription factors that regulate a wide range of cellular functions, such as cell growth and differentiation. Sp6, also called epiprofin, is specifically expressed in tooth germ, limb bud, and hair follicle, but there is little information on its function.To investigate the possible role of Sp6 in tooth development, first we established an Sp6-overproducing clone, CHA9, and analyzed the features of the cell, including cell proliferation and gene expression. The parental cells of CHA9 are the ameloblast-lineage G5 cells that we previously established from rat dental epithelia of lower incisor. Sp6 overproduction accelerated cell proliferation and induced the expression of ameloblastin mRNA, a marker of ameloblast differentiation. Second, we performed genome-wide screening of Sp6 target genes by microarray analysis. Out of a total 20,450 genes, 448 genes were up-regulated and 500 genes were down-regulated by Sp6. We found the expression of follistatin, a BMP antagonist, to be 22.4-fold lower in CHA9 than in control cells. Transfection of the Sp6-antisense construct into CHA9 cells restored follistatin expression back to equivalent levels seen in control cells, indicating that Sp6 regulates follistatin gene expression in ameloblasts.Our findings demonstrate that the follistatin gene is one of the Sp6 target genes in ameloblasts and suggest that Sp6 promotes amelogenesis through inhibition of follistatin gene expression.  (日)    [継承]
キーワード (推奨): 1. (英) Ameloblasts (日) (読) [継承]
2. (英) Amelogenesis (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) COS Cells (日) (読) [継承]
5. (英) Cell Differentiation (日) (読) [継承]
6. (英) Cercopithecus aethiops (日) (読) [継承]
7. (英) Clone Cells (日) (読) [継承]
8. (英) Dental Enamel Proteins (日) (読) [継承]
9. (英) Down-Regulation (日) (読) [継承]
10. (英) Follistatin (日) (読) [継承]
11. (英) Gene Expression Regulation, Developmental (日) (読) [継承]
12. (英) Kruppel-Like Transcription Factors (日) (読) [継承]
13. (英) Oligonucleotide Array Sequence Analysis (日) (読) [継承]
14. (英) Rabbits (日) (読) [継承]
15. (英) Rats (日) (読) [継承]
16. (英) Transfection (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Medical Investigation : JMI ([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)

ISSN (任意): 1343-1420
ISSN: 1343-1420 (pISSN: 1343-1420, eISSN: 1349-6867)
Title: The journal of medical investigation : JMI
Title(ISO): J. Med. Invest.
Supplier: 徳島大学
Publisher: Tokushima Daigaku Igakubu
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 55 [継承]
(必須): 1-2 [継承]
(必須): 87 98 [継承]
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年月日 (必須): 西暦 2008年 2月 初日 (平成 20年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.2152/jmi.55.87    (→Scopusで検索) [継承]
PMID (任意): 18319550    (→Scopusで検索) [継承]
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機関リポジトリ : 110844 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Molecular Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) CommentsCorrections.ErratumIn.RefSource: J Med Invest. 2008 Aug;55(3-4):303  (日)    [継承]

標準的な表示

和文冊子 ● Intan Ruspita, Keiko Miyoshi, Taro Muto, Kaori Abe, Taigo Horiguchi and Takafumi Noma : Sp6 downregulation of follistatin gene expression in ameloblasts., The Journal of Medical Investigation : JMI, Vol.55, No.1-2, 87-98, 2008.
欧文冊子 ● Intan Ruspita, Keiko Miyoshi, Taro Muto, Kaori Abe, Taigo Horiguchi and Takafumi Noma : Sp6 downregulation of follistatin gene expression in ameloblasts., The Journal of Medical Investigation : JMI, Vol.55, No.1-2, 87-98, 2008.

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