| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Terai Tomoya (日) (読)
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| 2. | 西村 範行
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| 3. | (英) Kanda Ikuno (日) (読)
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| 4. | 安井 夏生
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| 5. | 佐々木 卓也 ([徳島大学])
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| ○題名 (必須): | □ | (英) JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin. (日)
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| ○要約 (任意): | □ | (英) The dynamic turnover of tight junctions (TJs) is essential for epithelial-mesenchymal transitions and/or mesenchymal-epithelial transitions during epithelial morphogenesis. We previously demonstrated that Rab13 specifically mediates the endocytic recycling of occludin. Here, we identified MICAL-L2 (molecule interacting with CasL-like 2) as a novel Rab13-binding protein. Immunoprecipitation and immunofluorescence microscopy showed that MICAL-L2 specifically bound to the GTP-bound form of Rab13 via its C terminus, which contained a coiled-coil domain, and localized at TJs in epithelial MTD-1A cells. Recycling assay demonstrated that a MICAL-L2 mutant lacking the Rab13-binding domain (MICAL-L2-N) specifically inhibited the endocytic recycling of occludin but not transferrin receptor. Ca2+ switch assay further revealed that MICAL-L2-N as well as Rab13 Q67L inhibited the recruitment of occludin to the plasma membrane, the development of transepithelial electrical resistance, and the formation of a paracellular diffusion barrier. MICAL-L2 was displaced from TJs upon actin depolymerization and was distributed along radiating actin cables and stress fibers in Ca2+-depleted MTD-1A and fibroblastic NIH3T3 cells, respectively. These results suggest that MICAL-L2 mediates the endocytic recycling of occludin and the formation of functional TJs by linking Rab13 to actin cytoskeleton. We rename MICAL-L2 as JRAB (junctional Rab13-binding protein). (日)
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| ○キーワード (推奨): | 1. | (英) Actin Cytoskeleton (日) (読)
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| 2. | (英) Amino Acid Sequence (日) (読)
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| 3. | (英) Animals (日) (読)
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| 4. | (英) Calcium (日) (読)
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| 5. | (英) Cytoskeletal Proteins (日) (読)
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| 6. | (英) Dogs (日) (読)
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| 7. | (英) Endocytosis (日) (読)
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| 8. | (英) Epithelium (日) (読)
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| 9. | (英) Fibroblasts (日) (読)
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| 10. | (英) Membrane Proteins (日) (読)
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| 11. | (英) Mice (日) (読)
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| 12. | (英) Molecular Sequence Data (日) (読)
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| 13. | (英) Mutation (日) (読)
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| 14. | (英) NIH 3T3 Cells (日) (読)
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| 15. | (英) Protein Structure, Tertiary (日) (読)
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| 16. | (英) Tight Junctions (日) (読)
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| 17. | (英) rab GTP-Binding Proteins (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Molecular Biology of the Cell (The American Society for Cell Biology)
(pISSN: 1059-1524, eISSN: 1939-4586)
| ○ISSN (任意): | □ | 1059-1524
ISSN: 1059-1524
(pISSN: 1059-1524, eISSN: 1939-4586) Title: Molecular biology of the cellTitle(ISO): Mol Biol CellPublisher: American Society for Cell Biology (NLM Catalog)
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| ○巻 (必須): | □ | 17
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| ○号 (必須): | □ | 5
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| ○頁 (必須): | □ | 2465 2475
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2006年 5月 初日 (平成 18年 5月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1091/mbc.E05-09-0826 (→Scopusで検索)
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| ○PMID (任意): | □ | 16525024 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biochemistry, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770-8503, Japan. (日)
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| 2. | (英) Article.DataBankList.DataBank.DataBankName: GENBANK (日)
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| 3. | (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB182579 (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 5. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 6. | (英) OtherID: PMC1446078 (日)
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