『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=189963EID:189963, Map:0, LastModified:2012年9月2日(日) 19:26:53, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[佐々木 卓也], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Terai Tomoya (日) (読)
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2.西村 範行
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3. (英) Kanda Ikuno (日) (読)
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4.安井 夏生
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5.佐々木 卓也 ([徳島大学])
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題名 (必須): (英) JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The dynamic turnover of tight junctions (TJs) is essential for epithelial-mesenchymal transitions and/or mesenchymal-epithelial transitions during epithelial morphogenesis. We previously demonstrated that Rab13 specifically mediates the endocytic recycling of occludin. Here, we identified MICAL-L2 (molecule interacting with CasL-like 2) as a novel Rab13-binding protein. Immunoprecipitation and immunofluorescence microscopy showed that MICAL-L2 specifically bound to the GTP-bound form of Rab13 via its C terminus, which contained a coiled-coil domain, and localized at TJs in epithelial MTD-1A cells. Recycling assay demonstrated that a MICAL-L2 mutant lacking the Rab13-binding domain (MICAL-L2-N) specifically inhibited the endocytic recycling of occludin but not transferrin receptor. Ca2+ switch assay further revealed that MICAL-L2-N as well as Rab13 Q67L inhibited the recruitment of occludin to the plasma membrane, the development of transepithelial electrical resistance, and the formation of a paracellular diffusion barrier. MICAL-L2 was displaced from TJs upon actin depolymerization and was distributed along radiating actin cables and stress fibers in Ca2+-depleted MTD-1A and fibroblastic NIH3T3 cells, respectively. These results suggest that MICAL-L2 mediates the endocytic recycling of occludin and the formation of functional TJs by linking Rab13 to actin cytoskeleton. We rename MICAL-L2 as JRAB (junctional Rab13-binding protein).  (日)    [継承]
キーワード (推奨): 1. (英) Actin Cytoskeleton (日) (読) [継承]
2. (英) Amino Acid Sequence (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Calcium (日) (読) [継承]
5. (英) Cytoskeletal Proteins (日) (読) [継承]
6. (英) Dogs (日) (読) [継承]
7. (英) Endocytosis (日) (読) [継承]
8. (英) Epithelium (日) (読) [継承]
9. (英) Fibroblasts (日) (読) [継承]
10. (英) Membrane Proteins (日) (読) [継承]
11. (英) Mice (日) (読) [継承]
12. (英) Molecular Sequence Data (日) (読) [継承]
13. (英) Mutation (日) (読) [継承]
14. (英) NIH 3T3 Cells (日) (読) [継承]
15. (英) Protein Structure, Tertiary (日) (読) [継承]
16. (英) Tight Junctions (日) (読) [継承]
17. (英) rab GTP-Binding Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular Biology of the Cell (The American Society for Cell Biology)
(pISSN: 1059-1524, eISSN: 1939-4586)

ISSN (任意): 1059-1524
ISSN: 1059-1524 (pISSN: 1059-1524, eISSN: 1939-4586)
Title: Molecular biology of the cell
Title(ISO): Mol Biol Cell
Publisher: American Society for Cell Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 5 [継承]
(必須): 2465 2475 [継承]
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年月日 (必須): 西暦 2006年 5月 初日 (平成 18年 5月 初日) [継承]
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DOI (任意): 10.1091/mbc.E05-09-0826    (→Scopusで検索) [継承]
PMID (任意): 16525024    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biochemistry, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770-8503, Japan.  (日)    [継承]
2.(英) Article.DataBankList.DataBank.DataBankName: GENBANK  (日)    [継承]
3.(英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB182579  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
5.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
6.(英) OtherID: PMC1446078  (日)    [継承]

標準的な表示

和文冊子 ● Tomoya Terai, Noriyuki Nishimura, Ikuno Kanda, Natsuo Yasui and Takuya Sasaki : JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin., Molecular Biology of the Cell, Vol.17, No.5, 2465-2475, 2006.
欧文冊子 ● Tomoya Terai, Noriyuki Nishimura, Ikuno Kanda, Natsuo Yasui and Takuya Sasaki : JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin., Molecular Biology of the Cell, Vol.17, No.5, 2465-2475, 2006.

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