『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=189894)

EID=189894EID:189894, Map:0, LastModified:2012年10月9日(火) 18:27:08, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[佐々木 卓也], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Nakatsuji Hiroyoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.西村 範行
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Yamamura Rie (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Kanayama Hiro-omi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.佐々木 卓也 ([徳島大学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Tight junctions (TJs) are cell-cell adhesive structures that undergo continuous remodeling. We previously demonstrated that Rab13 and a junctional Rab13-binding protein (JRAB)/molecule interacting with CasL-like 2 (MICAL-L2) localized at TJs and mediated the endocytic recycling of the integral TJ protein occludin and the formation of functional TJs. Here, we investigated how JRAB/MICAL-L2 was targeted to TJs. Using a series of deletion mutants, we found the plasma membrane (PM)-targeting domain within JRAB/MICAL-L2. We then identified actinin-4, which was originally isolated as an actin-binding protein associated with cell motility and cancer invasion/metastasis, as a binding protein for the PM-targeting domain of JRAB/MICAL-L2, using a yeast two-hybrid system. Actinin-4 was colocalized with JRAB/MICAL-L2 at cell-cell junctions and linked JRAB/MICAL-L2 to F-actin. Although actinin-4 bound to JRAB/MICAL-L2 without Rab13, the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13 activation. Depletion of actinin-4 by using small interfering RNA inhibited the recruitment of occludin to TJs during the Ca(2+) switch. During the epithelial polarization after replating, JRAB/MICAL-L2 was recruited from the cytosol to cell-cell junctions. This JRAB/MICAL-L2 recruitment as well as the formation of functional TJs was delayed in actinin-4-depleted cells. These results indicate that actinin-4 is involved in recruiting JRAB/MICAL-L2 to cell-cell junctions and forming functional TJs.  (日)    [継承]
キーワード (推奨): 1. (英) Actinin (日) (読) [継承]
2. (英) Actins (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Base Sequence (日) (読) [継承]
5. (英) Binding Sites (日) (読) [継承]
6. (英) Calcium (日) (読) [継承]
7. (英) Cell Line (日) (読) [継承]
8. (英) Cell Polarity (日) (読) [継承]
9. (英) Cricetinae (日) (読) [継承]
10. (英) Cytoskeletal Proteins (日) (読) [継承]
11. (英) Epithelial Cells (日) (読) [継承]
12. (英) Genetic Vectors (日) (読) [継承]
13. (英) Intercellular Junctions (日) (読) [継承]
14. (英) Membrane Proteins (日) (読) [継承]
15. (英) Mice (日) (読) [継承]
16. (英) Microfilament Proteins (日) (読) [継承]
17. (英) Plasmids (日) (読) [継承]
18. (英) Protein Binding (日) (読) [継承]
19. (英) Protein Structure, Tertiary (日) (読) [継承]
20. (英) RNA, Small Interfering (日) (読) [継承]
21. (英) Recombinant Proteins (日) (読) [継承]
22. (英) Sequence Deletion (日) (読) [継承]
23. (英) Tight Junctions (日) (読) [継承]
24. (英) Transfection (日) (読) [継承]
25. (英) Two-Hybrid System Techniques (日) (読) [継承]
26. (英) rab GTP-Binding Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular and Cellular Biology ([アメリカ微生物学会])
(pISSN: 0270-7306, eISSN: 1098-5549)

ISSN (任意): 1098-5549
ISSN: 0270-7306 (pISSN: 0270-7306, eISSN: 1098-5549)
Title: Molecular and cellular biology
Title(ISO): Mol Cell Biol
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 28 [継承]
(必須): 10 [継承]
(必須): 3324 3335 [継承]
都市 (任意):
年月日 (必須): 西暦 2008年 5月 初日 (平成 20年 5月 初日) [継承]
URL (任意):
DOI (任意): 10.1128/MCB.00144-08    (→Scopusで検索) [継承]
PMID (任意): 18332111    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC2423145  (日)    [継承]

標準的な表示

和文冊子 ● Hiroyoshi Nakatsuji, Noriyuki Nishimura, Rie Yamamura, Hiro-omi Kanayama and Takuya Sasaki : Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions., Molecular and Cellular Biology, Vol.28, No.10, 3324-3335, 2008.
欧文冊子 ● Hiroyoshi Nakatsuji, Noriyuki Nishimura, Rie Yamamura, Hiro-omi Kanayama and Takuya Sasaki : Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions., Molecular and Cellular Biology, Vol.28, No.10, 3324-3335, 2008.

関連情報

Number of session users = 4, LA = 1.00, Max(EID) = 374082, Max(EOID) = 1001537.