『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=189887)

EID=189887EID:189887, Map:0, LastModified:2012年10月16日(火) 12:59:04, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[佐々木 卓也], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.西村 範行
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2.佐々木 卓也 ([徳島大学])
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[継承]
題名 (必須): (英) Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The dynamic turnover of adherens junctions (AJs) and tight junctions (TJs) is essential for epithelial morphogenesis during normal development and differentiation. Although the endocytic recycling of E-cadherin is characterized and implicated in AJ turnover, the molecular basis for TJ turnover is poorly understood. Occludin and claudins are distinct transmembrane proteins localized to the TJs. Although claudins are an indispensable structural component of TJ strands, depletion of occludin in mice reveals well-developed TJ strands and complex histological abnormalities. To examine the intracellular transport of transmembrane proteins to and from the cell surface, cell-surface biotinylation is a proven powerful method. Using this method, we successfully demonstrated that occludin was endocytosed and recycled back to the cell surface in both fibroblastic baby hamster kidney (BHK) and epithelial MTD-1A cells. The endocytic recycling of occludin as well as the formation of functional TJs was dependent on Rab13 and a junctional Rab13-binding protein (JRAB)/molecule interacting with CasL-like 2 (MICAL-L2). We describe the method to study the intracellular transport of occludin to and from the cell surface in both fibroblastic and epithelial cells.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Biological Assay (日) (読) [継承]
3. (英) Biotinylation (日) (読) [継承]
4. (英) Cell Line (日) (読) [継承]
5. (英) Cell Line, Tumor (日) (読) [継承]
6. (英) Cell Membrane (日) (読) [継承]
7. (英) Cricetinae (日) (読) [継承]
8. (英) Cytoskeletal Proteins (日) (読) [継承]
9. (英) Endocytosis (日) (読) [継承]
10. (英) Epithelial Cells (日) (読) [継承]
11. (英) Fibroblasts (日) (読) [継承]
12. (英) Humans (日) (読) [継承]
13. (英) Membrane Proteins (日) (読) [継承]
14. (英) Mice (日) (読) [継承]
15. (英) Recombinant Proteins (日) (読) [継承]
16. (英) Tight Junctions (日) (読) [継承]
17. (英) rab GTP-Binding Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Methods in Molecular Biology ([Springer])
(pISSN: 1064-3745, eISSN: 1940-6029)

ISSN (任意): 1064-3745
ISSN: 1064-3745 (pISSN: 1064-3745, eISSN: 1940-6029)
Title: Methods in molecular biology (Clifton, N.J.)
Title(ISO): Methods Mol Biol
Publisher: Humana Press, Inc.
 (NLM Catalog  (Scopus (Scopus information is found. [need login])
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年月日 (必須): 西暦 2008年 2月 初日 (平成 20年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.1007/978-1-59745-178-9_7    (→Scopusで検索) [継承]
PMID (任意): 18369939    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Noriyuki Nishimura and Takuya Sasaki : Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin., Methods in Molecular Biology, Vol.440, 89-96, 2008.
欧文冊子 ● Noriyuki Nishimura and Takuya Sasaki : Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin., Methods in Molecular Biology, Vol.440, 89-96, 2008.

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